PMID- 28912871
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220408
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 14
IP  - 4
DP  - 2017 Oct
TI  - Paeonol enhances the sensitivity of human ovarian cancer cells to 
      radiotherapy-induced apoptosis due to downregulation of the 
      phosphatidylinositol-3-kinase/Akt/phosphatase and tensin homolog pathway and 
      inhibition of vascular endothelial growth factor.
PG  - 3213-3220
LID - 10.3892/etm.2017.4877 [doi]
AB  - Radiotherapy is a vital and effective method to treat solid tumors. However, in 
      many tumor types, development of resistance of cancer cells and cytotoxicity in 
      normal tissues presents a major therapeutic problem. It is therefore crucial to 
      identify and develop novel sensitizing agents that may improve the response to 
      radiation therapy without causing any adverse effects. The present study aimed to 
      investigate whether paeonol, a bioactive flavonoid, was able to confer 
      sensitivity to radiation in human ovarian cancer cells. The human ovarian cancer 
      cell lines SKOV-3 and OVCAR-3 were exposed to varying doses of radiation (2, 4 or 
      6 Gy) in the presence or absence of paeonol (25, 50 or 100 microM). Radiosensitivity 
      was assessed by measuring cell viability using a CCK-8 assay and Annexin V/PI 
      staining. Expression of vascular endothelial growth factor (VEGF), hypoxia 
      inducible factor-1alpha (HIF-1alpha), proteins of the phosphatidylinositol-3-kinase 
      (PI3K)/Akt pathway and apoptotic pathway proteins [caspase-3, Bcl-2-associated 
      death promoter, B-cell lymphoma (Bcl)-2, Bcl-2-associated X and Bcl-extra large 
      (Bcl-xL)] were also assessed. Paeonol treatment enhanced apoptosis of SKOV-3 and 
      OVCAR-3 cells that were exposed to radiation. The expression of Bcl-2 and Bcl-xL 
      were markedly upregulated in these cells. Treatment with paeonol concentrations 
      of 50 and 100 microM caused a significant downregulation of VEGF, HIF-1alpha and PI3K/Akt 
      pathway proteins. Paeonol effectively enhanced the sensitivity of ovarian cancer 
      cells to radiation by significantly altering regulation of the proteins of the 
      PI3K/Akt pathway, in addition to downregulating VEGF and HIF-1alpha.
FAU - Zhou, Hai-Mei
AU  - Zhou HM
AD  - Department of Obstetrics and Gynecology, The Affiliated Hospital of Shandong 
      Medical College, Linyi, Shandong 276401, P.R. China.
FAU - Sun, Qin-Xiang
AU  - Sun QX
AD  - Department of Internal Medicine, The Affiliated Hospital of Shandong Medical 
      College, Linyi, Shandong 276401, P.R. China.
FAU - Cheng, Yan
AU  - Cheng Y
AD  - Department of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital 
      Affiliated To Fudan University, Shanghai 200090, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170803
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5585758
OTO - NOTNLM
OT  - apoptosis
OT  - ovarian cancer
OT  - paeonol
OT  - phosphatidylinositol-3-kinase/Akt pathway
OT  - radiotherapy
OT  - vascular endothelial growth factor
EDAT- 2017/09/16 06:00
MHDA- 2017/09/16 06:01
PMCR- 2017/08/03
CRDT- 2017/09/16 06:00
PHST- 2015/11/17 00:00 [received]
PHST- 2016/11/18 00:00 [accepted]
PHST- 2017/09/16 06:00 [entrez]
PHST- 2017/09/16 06:00 [pubmed]
PHST- 2017/09/16 06:01 [medline]
PHST- 2017/08/03 00:00 [pmc-release]
AID - ETM-0-0-4877 [pii]
AID - 10.3892/etm.2017.4877 [doi]
PST - ppublish
SO  - Exp Ther Med. 2017 Oct;14(4):3213-3220. doi: 10.3892/etm.2017.4877. Epub 2017 Aug 
      3.