PMID- 28914471
OWN - NLM
STAT- MEDLINE
DCOM- 20171212
LR  - 20210109
IS  - 1349-7006 (Electronic)
IS  - 1347-9032 (Print)
IS  - 1347-9032 (Linking)
VI  - 108
IP  - 12
DP  - 2017 Dec
TI  - WASH overexpression enhances cancer stem cell properties and correlates with poor 
      prognosis of esophageal carcinoma.
PG  - 2358-2365
LID - 10.1111/cas.13400 [doi]
AB  - There is increasing evidence that cytoskeleton remodeling is involved in cancer 
      progression. Wiskott-Aldrich syndrome protein (WASP) family represents a key 
      regulator of actin cytoskeleton remodeling. However, the underlying mechanism of 
      the WASP family in cancer progression remains elusive. Here, we studied the role 
      of WASP and SCAR Homolog (WASH), a recently identified WASP family member, in 
      human esophageal squamous cell carcinoma (ESCC). Using three human ESCC cell 
      lines, we found that WASH expression was significantly elevated in cancer 
      stem-like cells enriched by sphere formation assay. WASH knockdown decreased the 
      sphere-forming capacity of esophageal cancer cells whereas WASH over-expression 
      exhibited the opposite effect. Mechanistically, we identified interleukin-8 
      (IL-8) as a key downstream target of WASH. IL-8 knockdown completely attenuated 
      tumor sphere formation induced by WASH overexpression. WASH knockdown also 
      delayed the growth of human ESCC xenografts in BALB/c nude mice. Importantly, 
      high WASH levels were associated with poor clinical prognosis in a total of 145 
      human ESCC tissues. Collectively, our results suggest an essential role of the 
      WASH/IL-8 pathway in human ESCC by maintaining the stemness of cancer cells. 
      Hence, targeting this pathway might represent a promising strategy to control 
      human esophageal carcinoma.
CI  - (c) 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd 
      on behalf of Japanese Cancer Association.
FAU - Huang, Lan
AU  - Huang L
AUID- ORCID: 0000-0001-6556-4067
AD  - Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Lian, Jingyao
AU  - Lian J
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Chen, Xinfeng
AU  - Chen X
AD  - Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Qin, Guohui
AU  - Qin G
AD  - Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Zheng, Yujia
AU  - Zheng Y
AD  - Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20170926
PL  - England
TA  - Cancer Sci
JT  - Cancer science
JID - 101168776
RN  - 0 (Microfilament Proteins)
RN  - 0 (WASH protein, human)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/*metabolism/mortality/*pathology
MH  - Esophageal Neoplasms/*metabolism/mortality/*pathology
MH  - Esophageal Squamous Cell Carcinoma
MH  - Heterografts
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Microfilament Proteins/*metabolism
MH  - Neoplastic Stem Cells/*metabolism/*pathology
MH  - Prognosis
PMC - PMC5715296
OTO - NOTNLM
OT  - Cancer stem-like cell
OT  - Wiskott-Aldrich syndrome protein and SCAR Homolog (WASH)
OT  - esophageal squamous cell carcinoma
OT  - interleukin-8
OT  - prognosis
EDAT- 2017/09/16 06:00
MHDA- 2017/12/13 06:00
PMCR- 2017/12/01
CRDT- 2017/09/16 06:00
PHST- 2017/07/19 00:00 [received]
PHST- 2017/09/07 00:00 [revised]
PHST- 2017/09/12 00:00 [accepted]
PHST- 2017/09/16 06:00 [pubmed]
PHST- 2017/12/13 06:00 [medline]
PHST- 2017/09/16 06:00 [entrez]
PHST- 2017/12/01 00:00 [pmc-release]
AID - CAS13400 [pii]
AID - 10.1111/cas.13400 [doi]
PST - ppublish
SO  - Cancer Sci. 2017 Dec;108(12):2358-2365. doi: 10.1111/cas.13400. Epub 2017 Sep 26.