PMID- 28915507
OWN - NLM
STAT- MEDLINE
DCOM- 20171013
LR  - 20171120
IS  - 1096-0953 (Electronic)
IS  - 0013-9351 (Linking)
VI  - 159
DP  - 2017 Nov
TI  - Ambient fine particulate matters induce cell death and inflammatory response by 
      influencing mitochondria function in human corneal epithelial cells.
PG  - 595-605
LID - S0013-9351(17)30448-6 [pii]
LID - 10.1016/j.envres.2017.08.044 [doi]
AB  - Ambient fine particulate matter (AFP) is a main risk factor for the cornea as 
      ultraviolet light. However, the mechanism of corneal damage following exposure to 
      AFP has been poorly understood. In this study, we first confirmed that AFP can 
      penetrate the cornea of mice, considering that two-dimensional cell culture 
      systems are limited in reflecting the situation in vivo. Then, we investigated 
      the toxic mechanism using human corneal epithelial (HCET) cells. At 24h after 
      exposure, AFP located within the autophagosome-like vacuoles, and cell 
      proliferation was clearly inhibited in all the tested concentration. Production 
      of ROS and NO and secretion of pro-inflammatory cytokines were elevated in a 
      dose-dependent manner. Additionally, conversion of LC3B from I-type to II-type 
      and activation of caspase cascade which show autophagic- and apoptotic cell 
      death, respectively, were observed in cells exposed to AFP. Furthermore, AFP 
      decreased mitochondrial volume, inhibited ATP production, and altered the 
      expression of metabolism-related genes. Taken together, we suggest that AFP 
      induces cell death and inflammatory response by influencing mitochondrial 
      function in HCET cells. In addition, we recommend that stringent air quality 
      regulations are needed for eye health.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Park, Eun-Jung
AU  - Park EJ
AD  - Department of Brain Science, Ajou University School of Medicine, Suwon, Republic 
      of Korea. Electronic address: pejtoxic@hanmail.net.
FAU - Chae, Jae-Byoung
AU  - Chae JB
AD  - Department of Medical Science, Konyang University, Daejeon, Republic of Korea.
FAU - Lyu, Jungmook
AU  - Lyu J
AD  - Department of Medical Science, Konyang University, Daejeon, Republic of Korea.
FAU - Yoon, Cheolho
AU  - Yoon C
AD  - Seoul Center, Korea Basic Science Institute, Seoul 126-16, Republic of Korea.
FAU - Kim, Sanghwa
AU  - Kim S
AD  - College of Veterinary Medicine, Seoul National University, Seoul 151-742, 
      Republic of Korea.
FAU - Yeom, Changjoo
AU  - Yeom C
AD  - Department of Chemical Engineering, Kwangwoon University, Seoul, Republic of 
      Korea.
FAU - Kim, Younghun
AU  - Kim Y
AD  - Department of Chemical Engineering, Kwangwoon University, Seoul, Republic of 
      Korea.
FAU - Chang, Jaerak
AU  - Chang J
AD  - Department of Brain Science, Ajou University School of Medicine, Suwon, Republic 
      of Korea; Department of Biomedical Sciences, Ajou University School of Medicine, 
      Suwon, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170912
PL  - Netherlands
TA  - Environ Res
JT  - Environmental research
JID - 0147621
RN  - 0 (Cytokines)
RN  - 0 (Particulate Matter)
RN  - 0 (Reactive Oxygen Species)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cells, Cultured
MH  - Cornea/*drug effects
MH  - Cytokines/metabolism
MH  - Epithelial Cells/drug effects
MH  - Epithelium, Corneal/drug effects
MH  - Mice
MH  - Nitric Oxide/metabolism
MH  - Particulate Matter/*toxicity
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Ambient fine particulate matter
OT  - Cornea
OT  - Eye
OT  - Mitochondria
OT  - Oxidative stress
EDAT- 2017/09/16 06:00
MHDA- 2017/10/14 06:00
CRDT- 2017/09/16 06:00
PHST- 2017/03/09 00:00 [received]
PHST- 2017/08/24 00:00 [revised]
PHST- 2017/08/26 00:00 [accepted]
PHST- 2017/09/16 06:00 [pubmed]
PHST- 2017/10/14 06:00 [medline]
PHST- 2017/09/16 06:00 [entrez]
AID - S0013-9351(17)30448-6 [pii]
AID - 10.1016/j.envres.2017.08.044 [doi]
PST - ppublish
SO  - Environ Res. 2017 Nov;159:595-605. doi: 10.1016/j.envres.2017.08.044. Epub 2017 
      Sep 12.