PMID- 28916394
OWN - NLM
STAT- MEDLINE
DCOM- 20171213
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1520
DP  - 2017 Oct 20
TI  - A useful strategy based on chromatographic data combined with quality-by-design 
      approach for food analysis applications. The case study of furanic derivatives in 
      sugarcane honey.
PG  - 117-126
LID - S0021-9673(17)31339-0 [pii]
LID - 10.1016/j.chroma.2017.09.019 [doi]
AB  - Sugarcane honey (SCH) is one of the Madeira Island products par excellence and it 
      is now popular worldwide. Its sui generis and peculiar sensory properties, 
      explained by a variety of volatile compounds including furanic derivatives (FDs), 
      arise mainly from manufacturing and storage conditions. A simple high-throughput 
      approach based on semi-automatic microextraction by packed sorbent (MEPS) 
      combined with ultra-high performance liquid chromatography (UHPLC) was developed 
      and validated for identification and quantification of target FDs in sugarcane 
      honey. A Quality-by-Design (QbD) approach was used as a powerful strategy to 
      optimize analytical conditions for high throughput analysis of FDs in complex 
      sugar-rich food matrices. The optimum point into MEPS-Method Operable Design: 
      Region (MODR) was obtained with R-CX sorbent, acetonitrile (ACN) as elution 
      solvent, three loading cycles and 500muL of sample volume. The optimum point into 
      UHPLC-MODR was obtained with a CORTECS column operating at a temperature of 50 degrees C, 
      ACN as eluent and a flow rate of 125muLmin(-1). The robustness was demonstrated by 
      Monte Carlo simulation and capability analysis for estimation of residual errors. 
      The concentration-response relationship for all FDs were described by polynomial 
      function models, being confirmed by Fisher variance (F-test). The% recoveries 
      were in a range of 91.9-112.1%. Good method precision was observed, yielding 
      relative standard deviations (RSDs) less than 4.9% for repeatability and 8.8% for 
      intermediate precision. The limits of quantitation for the analytes ranged from 
      30.6 to 737.7mugkg(-1). The MEPS(R-CX)/UHPLC(CORTECS)-PDA method revealed an 
      effective and potential analytical tool for SCH authenticity control based on 
      target analysis of FDs allowing a strict control and differentiation from other 
      similar or adulterated products.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Silva, Pedro
AU  - Silva P
AD  - CQM - Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 
      9020-105 Funchal, Portugal.
FAU - Silva, Catarina L
AU  - Silva CL
AD  - CQM - Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 
      9020-105 Funchal, Portugal.
FAU - Perestrelo, Rosa
AU  - Perestrelo R
AD  - CQM - Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 
      9020-105 Funchal, Portugal.
FAU - Nunes, Fernando M
AU  - Nunes FM
AD  - CQ-VR, Centro de Quimica - Vila Real, Departamento de Quimica, Universidade de 
      Tras-os-Montes e Alto Douro, 5001- 801 Vila Real, Portugal.
FAU - Camara, Jose S
AU  - Camara JS
AD  - CQM - Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 
      9020-105 Funchal, Portugal; Departamento de Quimica, Faculdade de Ciencias 
      Exactas e Engenharia, Universidade da Madeira, Campus da Penteada, 9020-105 
      Funchal, Portugal. Electronic address: jsc@uma.pt.
LA  - eng
PT  - Journal Article
DEP - 20170908
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
SB  - IM
MH  - *Chromatography, High Pressure Liquid
MH  - Food Analysis/instrumentation/*methods
MH  - Honey/*analysis/*standards
MH  - Limit of Detection
MH  - Portugal
MH  - Saccharum
MH  - Solid Phase Microextraction
OTO - NOTNLM
OT  - Furanic derivatives
OT  - MEPS
OT  - Quality-by-design
OT  - Sugarcane honey
OT  - UHPLC-PDA
EDAT- 2017/09/17 06:00
MHDA- 2017/12/14 06:00
CRDT- 2017/09/17 06:00
PHST- 2017/07/13 00:00 [received]
PHST- 2017/09/05 00:00 [revised]
PHST- 2017/09/07 00:00 [accepted]
PHST- 2017/09/17 06:00 [pubmed]
PHST- 2017/12/14 06:00 [medline]
PHST- 2017/09/17 06:00 [entrez]
AID - S0021-9673(17)31339-0 [pii]
AID - 10.1016/j.chroma.2017.09.019 [doi]
PST - ppublish
SO  - J Chromatogr A. 2017 Oct 20;1520:117-126. doi: 10.1016/j.chroma.2017.09.019. Epub 
      2017 Sep 8.