PMID- 28917183
OWN - NLM
STAT- MEDLINE
DCOM- 20180103
LR  - 20180103
IS  - 1878-562X (Electronic)
IS  - 1567-5394 (Linking)
VI  - 119
DP  - 2018 Feb
TI  - Visualisation of an nsPEF induced calcium wave using the genetically encoded 
      calcium indicator GCaMP in U87 human glioblastoma cells.
PG  - 68-75
LID - S1567-5394(17)30302-X [pii]
LID - 10.1016/j.bioelechem.2017.09.003 [doi]
AB  - Cytosolic, synthetic chemical calcium indicators are typically used to visualise 
      the rapid increase in intracellular calcium ion concentration that follows 
      nanosecond pulsed electric field (nsPEF) application. This study looks at the 
      application of genetically encoded calcium indicators (GECIs) to investigate the 
      spatiotemporal nature of nsPEF-induced calcium signals using fluorescent live 
      cell imaging. Calcium responses to 44kV/cm, 10ns pulses were observed in U87-MG 
      cells expressing either a plasma membrane targeted GECI (GCaMP5-G), or one 
      cytosolically expressed (GCaMP6-S), and compared to the response of cells loaded 
      with cytosolic or plasma membrane targeted chemical calcium indicators. 
      Application of 100 pulses, to cells containing plasma membrane targeted 
      indicators, revealed a wave of calcium across the cell initiating at the cathode 
      side. A similar spatial wave was not observed with cytosolic indicators with 
      mobile calcium buffering properties. The speed of the wave was related to pulse 
      application frequency and it was not propagated by calcium induced calcium 
      release.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Carr, Lynn
AU  - Carr L
AD  - Univ. Limoges, CNRS, XLIM, UMR 7252, F-87000 Limoges, France.
FAU - Bardet, Sylvia M
AU  - Bardet SM
AD  - Univ. Limoges, CNRS, XLIM, UMR 7252, F-87000 Limoges, France.
FAU - Arnaud-Cormos, Delia
AU  - Arnaud-Cormos D
AD  - Univ. Limoges, CNRS, XLIM, UMR 7252, F-87000 Limoges, France.
FAU - Leveque, Philippe
AU  - Leveque P
AD  - Univ. Limoges, CNRS, XLIM, UMR 7252, F-87000 Limoges, France.
FAU - O'Connor, Rodney P
AU  - O'Connor RP
AD  - IMT Mines Saint-Etienne, Centre CMP, Department of Bioelectronics, F - 13541 
      Gardanne, France. Electronic address: rodney.oconnor@emse.fr.
LA  - eng
PT  - Journal Article
DEP - 20170908
PL  - Netherlands
TA  - Bioelectrochemistry
JT  - Bioelectrochemistry (Amsterdam, Netherlands)
JID - 100953583
RN  - 0 (Fluorescent Dyes)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*metabolism
MH  - *Calcium Signaling
MH  - Cell Membrane/metabolism
MH  - Cell Survival
MH  - *Electricity
MH  - Fluorescent Dyes/*metabolism
MH  - *Genetic Engineering
MH  - Glioblastoma/*pathology
MH  - Humans
MH  - Intracellular Space/metabolism
MH  - Optical Imaging/*methods
OTO - NOTNLM
OT  - Calcium wave
OT  - GCaMP
OT  - Genetically encoded calcium indicator
OT  - Plasma membrane calcium indicators
OT  - nsPEF
EDAT- 2017/09/17 06:00
MHDA- 2018/01/04 06:00
CRDT- 2017/09/17 06:00
PHST- 2017/05/30 00:00 [received]
PHST- 2017/08/23 00:00 [revised]
PHST- 2017/09/07 00:00 [accepted]
PHST- 2017/09/17 06:00 [pubmed]
PHST- 2018/01/04 06:00 [medline]
PHST- 2017/09/17 06:00 [entrez]
AID - S1567-5394(17)30302-X [pii]
AID - 10.1016/j.bioelechem.2017.09.003 [doi]
PST - ppublish
SO  - Bioelectrochemistry. 2018 Feb;119:68-75. doi: 10.1016/j.bioelechem.2017.09.003. 
      Epub 2017 Sep 8.