PMID- 28917607
OWN - NLM
STAT- MEDLINE
DCOM- 20180705
LR  - 20180706
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 142
DP  - 2017 Nov
TI  - Selection of reliable reference genes for gene expression studies in Botrytis 
      cinerea.
PG  - 71-75
LID - S0167-7012(17)30247-6 [pii]
LID - 10.1016/j.mimet.2017.09.006 [doi]
AB  - Botrytis cinerea is an important plant pathogen causing grey mold disease in a 
      wide range of plant species. The aim of this study was to identify reliable 
      reference genes that can be used for the analysis of relative gene expression in 
      B. cinerea with quantitative real-time reverse transcription PCR (qRT-PCR). Six 
      commonly used housekeeping genes including actin (ACT), 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ubiquitin (UBQ), 
      ubiquitin-conjugating enzyme (UCE), alpha-tubulin (alpha-TUB) and beta-tubulin (beta-TUB) were 
      selected to test their expression stabilities in B. cinerea treated with 
      different concentration of oxalic acid (1, 5 and 10mM) and confronted with 
      antagonistic Trichoderma afroharzianum. Four in silico algorithms (geNorm, 
      BestKeeper, NormFinder and Comparative DeltaCt) were applied to evaluate the 
      expression stabilities of these genes, and the UBQ gene was identified as the 
      most stably expressed. It was used to normalize the expression levels of three 
      genes related to oxalic acid production (NADPH, VEL1 and OAH) when B. cinerea was 
      challenged by T. afroharzianum. The results of this study are useful for gene 
      expression analysis in B. cinerea.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Ren, He
AU  - Ren H
AD  - College of Life Science, Shandong University of Technology, Zibo 255000, Shandong 
      Province, People's Republic of China; Shandong Provincial Key Laboratory of 
      Applied Microbiology, Ecology Institute, Shandong Academy of Sciences, Jinan 
      250014, Shandong Province, People's Republic of China.
FAU - Wu, Xiaoqing
AU  - Wu X
AD  - Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, 
      Shandong Academy of Sciences, Jinan 250014, Shandong Province, People's Republic 
      of China.
FAU - Lyu, Yuping
AU  - Lyu Y
AD  - College of Life Science, Shandong University of Technology, Zibo 255000, Shandong 
      Province, People's Republic of China; Shandong Provincial Key Laboratory of 
      Applied Microbiology, Ecology Institute, Shandong Academy of Sciences, Jinan 
      250014, Shandong Province, People's Republic of China.
FAU - Zhou, Hongzi
AU  - Zhou H
AD  - Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, 
      Shandong Academy of Sciences, Jinan 250014, Shandong Province, People's Republic 
      of China.
FAU - Xie, Xueying
AU  - Xie X
AD  - Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, 
      Shandong Academy of Sciences, Jinan 250014, Shandong Province, People's Republic 
      of China.
FAU - Zhang, Xinjian
AU  - Zhang X
AD  - Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, 
      Shandong Academy of Sciences, Jinan 250014, Shandong Province, People's Republic 
      of China. Electronic address: zhangxj@sdas.org.
FAU - Yang, Hetong
AU  - Yang H
AD  - Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, 
      Shandong Academy of Sciences, Jinan 250014, Shandong Province, People's Republic 
      of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170914
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Actins)
RN  - 0 (Tubulin)
RN  - 0 (Ubiquitin)
RN  - 53-59-8 (NADP)
RN  - 9E7R5L6H31 (Oxalic Acid)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 3.- (Hydrolases)
SB  - IM
MH  - Actins/genetics
MH  - Botrytis/*genetics/*metabolism
MH  - Gene Expression Regulation, Fungal/genetics
MH  - Genes, Essential/*genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
MH  - Hydrolases/genetics
MH  - NADP/genetics
MH  - Oxalic Acid/*metabolism
MH  - Plant Diseases/microbiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Trichoderma/metabolism
MH  - Tubulin/genetics
MH  - Ubiquitin/genetics
MH  - Ubiquitin-Conjugating Enzymes/genetics
OTO - NOTNLM
OT  - Botrytis cinerea
OT  - Gene expression
OT  - Reference gene
OT  - Trichoderma afroharzianum
EDAT- 2017/09/18 06:00
MHDA- 2018/07/06 06:00
CRDT- 2017/09/18 06:00
PHST- 2017/04/06 00:00 [received]
PHST- 2017/08/30 00:00 [revised]
PHST- 2017/09/10 00:00 [accepted]
PHST- 2017/09/18 06:00 [pubmed]
PHST- 2018/07/06 06:00 [medline]
PHST- 2017/09/18 06:00 [entrez]
AID - S0167-7012(17)30247-6 [pii]
AID - 10.1016/j.mimet.2017.09.006 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2017 Nov;142:71-75. doi: 10.1016/j.mimet.2017.09.006. Epub 
      2017 Sep 14.