PMID- 28917760
OWN - NLM
STAT- MEDLINE
DCOM- 20180906
LR  - 20180906
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 176
DP  - 2018 Jan 1
TI  - Rapid and sensitive serum glucose determination using chemical labeling coupled 
      with black phosphorus-assisted laser desorption/ionization time-of-flight mass 
      spectrometry.
PG  - 344-349
LID - S0039-9140(17)30883-4 [pii]
LID - 10.1016/j.talanta.2017.08.055 [doi]
AB  - Monitoring the concentration of blood glucose in patients is a key component of 
      good medical diagnoses. Therefore, developing an accurate, rapid and sensitive 
      strategy for monitoring blood glucose is of vital importance. We proposed a 
      strategy for serum glucose determination combining 2-(4-boronobenzyl) 
      isoquinolin-2-ium bromide chemical labeling with black phosphorus assisted laser 
      desorption ionization-time of flight mass spectrometry (CL-BP/ALDI-TOF MS). The 
      entire analytical process consisted of 1min of protein precipitation and 3min of 
      chemical labeling in a microwave oven prior to the BP/ALDI-TOF MS analysis. The 
      analysis can be completed in 5min with high throughput and extremely low sample 
      consumption. Good linearity for glucose was obtained with a correlation 
      coefficient (R) of 0.9986. The limit of detection (LOD) and limit of 
      quantification (LOQ) were 11.5 fmol and 37.5 fmol, respectively. Satisfied 
      reproducibility and reliability were gained by evaluation of the intra- and 
      inter-day precisions with relative standard deviations (RSDs) less than 7.2% and 
      relative recoveries ranging from 87.1% to 108.1%, respectively. The proposed 
      strategy was also applied for the analysis of endogenous glucose in various serum 
      samples and the results were consistent with those obtained using the hexokinase 
      method in a clinical laboratory. Considering the results, the proposed 
      CL-BP/ALDI-TOF MS strategy has proven to be reliable, fast, and sensitive for 
      quantitative analysis of serum glucose.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Wang, Qing
AU  - Wang Q
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, 
      People's Republic of China.
FAU - Yu, Lei
AU  - Yu L
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, 
      People's Republic of China.
FAU - Qi, Chu-Bo
AU  - Qi CB
AD  - Department of Pathology, Hubei Cancer Hospital, Wuhan, Hubei 430079, People's 
      Republic of China.
FAU - Ding, Jun
AU  - Ding J
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, 
      People's Republic of China.
FAU - He, Xiao-Mei
AU  - He XM
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, 
      People's Republic of China.
FAU - Wang, Ren-Qi
AU  - Wang RQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, 
      People's Republic of China; College of Chemistry and Chemical Engineering, 
      Lanzhou, Gansu 730000, People's Republic of China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, 
      People's Republic of China. Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170816
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Blood Glucose)
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - Blood Glucose/*analysis
MH  - Humans
MH  - Lasers
MH  - Phosphorus
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
OTO - NOTNLM
OT  - Black phosphorus matrix
OT  - Chemical labeling
OT  - Matrix assisted laser desorption ionization-time of flight mass spectrometry
OT  - Serum glucose
EDAT- 2017/09/18 06:00
MHDA- 2018/09/07 06:00
CRDT- 2017/09/18 06:00
PHST- 2017/06/06 00:00 [received]
PHST- 2017/08/11 00:00 [revised]
PHST- 2017/08/15 00:00 [accepted]
PHST- 2017/09/18 06:00 [entrez]
PHST- 2017/09/18 06:00 [pubmed]
PHST- 2018/09/07 06:00 [medline]
AID - S0039-9140(17)30883-4 [pii]
AID - 10.1016/j.talanta.2017.08.055 [doi]
PST - ppublish
SO  - Talanta. 2018 Jan 1;176:344-349. doi: 10.1016/j.talanta.2017.08.055. Epub 2017 
      Aug 16.