PMID- 28917762
OWN - NLM
STAT- MEDLINE
DCOM- 20180906
LR  - 20191210
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 176
DP  - 2018 Jan 1
TI  - Full validation of a method for the determination of drugs of abuse in 
      non-mineralized dental biofilm using liquid chromatography-tandem mass 
      spectrometry and application to postmortem samples.
PG  - 360-366
LID - S0039-9140(17)30867-6 [pii]
LID - 10.1016/j.talanta.2017.08.047 [doi]
AB  - Alternative matrices play a major role in postmortem forensic toxicology, 
      especially if common matrices (like body fluids or hair) are not available. 
      Incorporation of illicit and medicinal drugs into non-mineralized dental biofilm 
      (plaque) seems likely but has not been investigated so far. Analysis of plaque 
      could therefore extend the spectrum of potentially used matrices in postmortem 
      toxicology. For this reason, a rapid, simple and sensitive method for the 
      extraction, determination and quantification of ten drugs of abuse from plaque 
      using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and 
      fully validated. Amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine 
      (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), 
      3,4-methylenedioxyamphetamine (MDA), cocaine, benzoylecgonine, morphine, codeine 
      and 6-acetylmorphine were extracted from 2mg of dried and powdered plaque via 
      ultrasonication with acetonitrile. The extracts were analyzed on a 
      triple-quadrupole linear ion trap mass spectrometer in scheduled multiple 
      reaction monitoring mode (sMRM). The method was fully validated and proved 
      accurate, precise, selective and specific with satisfactory linearity within the 
      calibrated ranges. The lower limit of quantification was 10-15pgmg(-1) for all 
      compounds except for MDA (100pgmg(-1)) and amphetamine (200pgmg(-1)). The method 
      has been successfully applied to three authentic postmortem samples with known 
      drug history. Amphetamine, MDMA, cocaine, benzoylecgonine, morphine and codeine 
      could be detected in these cases in concentrations ranging from 18pgmg(-1) for 
      cocaine to 1400pgmg(-1) for amphetamine.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Henkel, Kerstin
AU  - Henkel K
AD  - Institute of Forensic Medicine, Forensic Toxicology, Medical Center - University 
      of Freiburg, Faculty of Medicine, University of Freiburg, Albertstr. 9, 79104 
      Freiburg, Germany; Hermann Staudinger Graduate School, University of Freiburg, 
      Hebelstr. 27, 79104 Freiburg, Germany.
FAU - Altenburger, Markus J
AU  - Altenburger MJ
AD  - Center for Dental Medicine, Department of Operative Dentistry and Periodontology, 
      Medical Center - University of Freiburg, Faculty of Medicine, University of 
      Freiburg, Hugstetter Str. 55, 79106 Freiburg, Germany.
FAU - Auwarter, Volker
AU  - Auwarter V
AD  - Institute of Forensic Medicine, Forensic Toxicology, Medical Center - University 
      of Freiburg, Faculty of Medicine, University of Freiburg, Albertstr. 9, 79104 
      Freiburg, Germany.
FAU - Neukamm, Merja A
AU  - Neukamm MA
AD  - Institute of Forensic Medicine, Forensic Toxicology, Medical Center - University 
      of Freiburg, Faculty of Medicine, University of Freiburg, Albertstr. 9, 79104 
      Freiburg, Germany. Electronic address: merja.neukamm@uniklinik-freiburg.de.
LA  - eng
PT  - Journal Article
DEP - 20170815
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Amphetamines)
RN  - 0 (Illicit Drugs)
RN  - 0 (Morphine Derivatives)
RN  - 5353I8I6YS (benzoylecgonine)
RN  - I5Y540LHVR (Cocaine)
SB  - IM
MH  - Amphetamines/analysis
MH  - *Biofilms
MH  - Chromatography, Liquid
MH  - Cocaine/analogs & derivatives/analysis
MH  - Dental Plaque/*chemistry/*microbiology
MH  - Humans
MH  - Illicit Drugs/*analysis
MH  - Morphine Derivatives/analysis
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Alternative matrices
OT  - Forensic toxicology
OT  - LC-MS/MS
OT  - Plaque
OT  - Postmortem toxicology
EDAT- 2017/09/18 06:00
MHDA- 2018/09/07 06:00
CRDT- 2017/09/18 06:00
PHST- 2017/02/14 00:00 [received]
PHST- 2017/08/09 00:00 [revised]
PHST- 2017/08/12 00:00 [accepted]
PHST- 2017/09/18 06:00 [entrez]
PHST- 2017/09/18 06:00 [pubmed]
PHST- 2018/09/07 06:00 [medline]
AID - S0039-9140(17)30867-6 [pii]
AID - 10.1016/j.talanta.2017.08.047 [doi]
PST - ppublish
SO  - Talanta. 2018 Jan 1;176:360-366. doi: 10.1016/j.talanta.2017.08.047. Epub 2017 
      Aug 15.