PMID- 28919967 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 1993-0771 (Print) IS - 1993-078X (Electronic) IS - 1993-0771 (Linking) VI - 11 IP - 2 DP - 2017 TI - Comparative analysis of chromosomes in the Palaearctic bush-crickets of tribe Pholidopterini (Orthoptera, Tettigoniinae). PG - 309-324 LID - 10.3897/CompCytogen.v11i2.12070 [doi] AB - The present study focused on the evolution of the karyotype in four genera of the tribe Pholidopterini: Eupholidoptera Maran, 1953, Parapholidoptera Maran, 1953, Pholidoptera Wesmael, 1838, Uvarovistia Maran, 1953. Chromosomes were analyzed using fluorescence in situ hybridization (FISH) with 18S rDNA and (TTAGG) (n) telomeric probes, and classical techniques, such as C-banding, silver impregnation and fluorochrome DAPI/CMA(3) staining. Most species retained the ancestral diploid chromosome number 2n = 31 (male) or 32 (female), while some of the taxa, especially a group of species within genus Pholidoptera, evolved a reduced chromosome number 2n = 29. All species show the same sex determination system X0/XX. In some taxa, a pericentric inversion has changed the morphology of the ancestral acrocentric X chromosome to the biarmed X. The rDNA loci coincided with active NORs and C-band/CG-rich segments. A comparison of the location of the single rDNA/NOR in the genus Pholidoptera suggests that reduced chromosome number results from Robertsonian translocation between two pairs of autosomes, one carrying the rDNA/NOR. The results constitute a step towards better understanding of the chromosomal reorganization and evolution within the tribe Phaneropterini and the whole subfamily Tettigoniinae. FAU - Warchalowska-Sliwa, Elzbieta AU - Warchalowska-Sliwa E AD - Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Slawkowska 17, 31-016 Krakow, Poland. FAU - Grzywacz, Beata AU - Grzywacz B AD - Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Slawkowska 17, 31-016 Krakow, Poland. FAU - Heller, Klaus-Gerhard AU - Heller KG AD - Grillenstieg 18, 39120 Magdeburg, Germany. FAU - Chobanov, Dragan P AU - Chobanov DP AD - Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1 Tsar Osvoboditel Boul., 1000 Sofia, Bulgaria. LA - eng PT - Journal Article DEP - 20170505 PL - Bulgaria TA - Comp Cytogenet JT - Comparative cytogenetics JID - 101503807 PMC - PMC5596980 OTO - NOTNLM OT - 18S rDNA OT - C-banding OT - FISH OT - NOR OT - Orthoptera OT - Pholidopterini OT - fluorochrome staining OT - karyotype OT - telomeric DNA EDAT- 2017/09/19 06:00 MHDA- 2017/09/19 06:01 PMCR- 2017/05/05 CRDT- 2017/09/19 06:00 PHST- 2017/02/02 00:00 [received] PHST- 2017/03/28 00:00 [accepted] PHST- 2017/09/19 06:00 [entrez] PHST- 2017/09/19 06:00 [pubmed] PHST- 2017/09/19 06:01 [medline] PHST- 2017/05/05 00:00 [pmc-release] AID - 10.3897/CompCytogen.v11i2.12070 [doi] PST - epublish SO - Comp Cytogenet. 2017 May 5;11(2):309-324. doi: 10.3897/CompCytogen.v11i2.12070. eCollection 2017.