PMID- 28921460
OWN - NLM
STAT- MEDLINE
DCOM- 20180831
LR  - 20201209
IS  - 1699-3055 (Electronic)
IS  - 1699-048X (Linking)
VI  - 20
IP  - 4
DP  - 2018 Apr
TI  - NFBD1/MDC1 participates in the regulation of proliferation and apoptosis in human 
      laryngeal squamous cell carcinoma.
PG  - 534-541
LID - 10.1007/s12094-017-1748-5 [doi]
AB  - PURPOSE: The objective of the study was to investigate the role of NFBD1 in the 
      proliferation and apoptosis of laryngeal squamous cell carcinoma (LSCC) cells. 
      METHODS: Immunohistochemistry (IHC) and qRT-PCR was employed to determine the 
      expressions of NFBD1 protein and mRNA in LSCC tissues and adjacent noncancerous 
      tissues. After the downregulation of NFBD1 expression, the colony formation 
      assay, MTS assay and apoptosis assay were used to investigate the changes in the 
      proliferation and apoptosis of Hep2 cells. The mechanisms by which silencing 
      NFBD1 promote apoptosis of Hep2 cells were examined by western blotting. 
      Furthermore, xenograft models were used to evaluate the proliferation of Hep2 
      cells in vivo. RESULTS: NFBD1 protein was upregulated in 55.6% of LSCC cancer 
      tissues compared with adjacent normal tissues (26.7%). NFBD1 knockdown in Hep2 
      cells significantly impacted proliferation and apoptosis, and silencing NFBD1 
      might promote apoptosis of Hep2 cells by activating the mitochondrial apoptotic 
      pathway. Xenograft models showed that silencing NFBD1 also significantly 
      inhibited tumor growth. CONCLUSIONS: Our data highlight that NFBD1 participates 
      in the regulation of proliferation and apoptosis in LSCC, and suggest that NFBD1 
      could be a promising therapy target.
FAU - Liu, X
AU  - Liu X
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Qiu, Z
AU  - Qiu Z
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Wang, Z
AU  - Wang Z
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Zuo, W
AU  - Zuo W
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Gong, Z
AU  - Gong Z
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Liu, C
AU  - Liu C
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Zeng, Q
AU  - Zeng Q
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Qian, Y
AU  - Qian Y
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Jiang, L
AU  - Jiang L
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Li, Y
AU  - Li Y
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Bu, Y
AU  - Bu Y
AD  - Department of Biochemistry and Molecular Biology, Molecular Medicine and Cancer 
      Research Center, Chongqing Medical University, Chongqing, 400016, China.
FAU - Hu, G
AU  - Hu G
AUID- ORCID: 0000-0002-6220-2084
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China. hghcq@sina.com.
LA  - eng
GR  - 81470676/National Natural Science Foundation of China/
GR  - 81271061/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20170918
PL  - Italy
TA  - Clin Transl Oncol
JT  - Clinical & translational oncology : official publication of the Federation of 
      Spanish Oncology Societies and of the National Cancer Institute of Mexico
JID - 101247119
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (MDC1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Apoptosis/physiology
MH  - Carcinoma, Squamous Cell/*pathology
MH  - Cell Cycle Proteins
MH  - Cell Proliferation/physiology
MH  - Female
MH  - Head and Neck Neoplasms/*pathology
MH  - Heterografts
MH  - Humans
MH  - Laryngeal Neoplasms/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Middle Aged
MH  - Nuclear Proteins/*metabolism
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Trans-Activators/*metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Cell cycle
OT  - Laryngeal squamous cell carcinoma (LSCC)
OT  - NFBD1/MDC1
EDAT- 2017/09/19 06:00
MHDA- 2018/09/01 06:00
CRDT- 2017/09/19 06:00
PHST- 2017/02/09 00:00 [received]
PHST- 2017/08/28 00:00 [accepted]
PHST- 2017/09/19 06:00 [pubmed]
PHST- 2018/09/01 06:00 [medline]
PHST- 2017/09/19 06:00 [entrez]
AID - 10.1007/s12094-017-1748-5 [pii]
AID - 10.1007/s12094-017-1748-5 [doi]
PST - ppublish
SO  - Clin Transl Oncol. 2018 Apr;20(4):534-541. doi: 10.1007/s12094-017-1748-5. Epub 
      2017 Sep 18.