PMID- 28921579
OWN - NLM
STAT- MEDLINE
DCOM- 20180223
LR  - 20180223
IS  - 1099-0801 (Electronic)
IS  - 0269-3879 (Linking)
VI  - 32
IP  - 2
DP  - 2018 Feb
TI  - Material basis studies of anti-Influenza A active ingredients in Tanreqing 
      Injection.
LID - 10.1002/bmc.4097 [doi]
AB  - Tanreqing Injection (TRQ) has been used primarily in treating infections of the 
      upper respiratory tract and serious influenza in China, as a classical compound 
      herbal recipe. TRQ had been demonstrated to have effects of clearing heat, 
      eliminating phlegm, detoxification, reducing inflammation and alleviating cough. 
      The survival rate, histopathology of lungs and viral titers in mice were 
      evaluated in this study to verify the curative effect of TRQ. However, there is 
      not enough information about the components. In the present study, a 
      high-performance and practical LC/QTOF/MS method was developed for 
      characterization and identification of the natural ingredients in TRQ. A total of 
      60 compounds, including 10 amino acids, 10 iridoid glucosides, 14 flavonoids, 13 
      other phenolic compounds, 10 steroid acids and three other compounds, were 
      characterized and identified. We also confirmed the material basis of 
      anti-Influenza A active ingredients in TRQ. Therefore, we have developed an 
      accurate analytical method. LC/QTOF/MS could be applied for identification the 
      complex components in traditional Chinese medicine.
CI  - Copyright (c) 2017 John Wiley & Sons, Ltd.
FAU - Zhu, Haiyan
AU  - Zhu H
AUID- ORCID: 0000-0002-8109-6678
AD  - Department of Microbiological and Biochemical Pharmacy, School of Pharmacy, Fudan 
      University, Shanghai, China.
FAU - Chen, Mingcang
AU  - Chen M
AD  - Shanghai Institutes of Materia Medica, Chinese Academy of Sciences, Shanghai, 
      China.
FAU - Shi, Xunlong
AU  - Shi X
AD  - Department of Microbiological and Biochemical Pharmacy, School of Pharmacy, Fudan 
      University, Shanghai, China.
FAU - Shi, Chenchen
AU  - Shi C
AD  - Department of Microbiological and Biochemical Pharmacy, School of Pharmacy, Fudan 
      University, Shanghai, China.
FAU - Huang, Chenggang
AU  - Huang C
AD  - Shanghai Institutes of Materia Medica, Chinese Academy of Sciences, Shanghai, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20171010
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Amino Acids)
RN  - 0 (Antiviral Agents)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 0 (Iridoid Glucosides)
RN  - 0 (tanreqing)
SB  - IM
MH  - Amino Acids/analysis/chemistry
MH  - Animals
MH  - Antiviral Agents/administration & dosage/*chemistry/pharmacology
MH  - Chromatography, Liquid
MH  - Drugs, Chinese Herbal/administration & dosage/*chemistry/pharmacology
MH  - Flavonoids/analysis/chemistry
MH  - Influenza A Virus, H1N1 Subtype/*drug effects
MH  - Iridoid Glucosides/analysis/chemistry
MH  - Lung/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Orthomyxoviridae Infections
MH  - Pneumonia, Viral
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Tandem Mass Spectrometry
MH  - Virus Replication/drug effects
OTO - NOTNLM
OT  - Influenza A virus
OT  - LC/QTOF/MS
OT  - Tanreqing Injection
OT  - antiviral research
OT  - component analysis
EDAT- 2017/09/19 06:00
MHDA- 2018/02/24 06:00
CRDT- 2017/09/19 06:00
PHST- 2017/06/06 00:00 [received]
PHST- 2017/08/10 00:00 [revised]
PHST- 2017/09/07 00:00 [accepted]
PHST- 2017/09/19 06:00 [pubmed]
PHST- 2018/02/24 06:00 [medline]
PHST- 2017/09/19 06:00 [entrez]
AID - 10.1002/bmc.4097 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2018 Feb;32(2). doi: 10.1002/bmc.4097. Epub 2017 Oct 10.