PMID- 28921745
OWN - NLM
STAT- MEDLINE
DCOM- 20180614
LR  - 20221207
IS  - 1600-0897 (Electronic)
IS  - 1046-7408 (Linking)
VI  - 78
IP  - 6
DP  - 2017 Dec
TI  - Transcription factor CCAAT/enhancer-binding protein-beta upregulates microRNA, 
      let-7f-1 in human endocervical cells.
LID - 10.1111/aji.12759 [doi]
AB  - PROBLEM: In endocervical epithelial cells (End1/E6E7), miRNA let-7f plays an 
      important role in the control of innate immunity. The underlying molecular 
      mechanism for let-7f regulation in these cells remains largely unclear. METHODS 
      OF STUDY: let-7f was knocked down in End1/E6E7 cells using siRNA, and 
      differential gene expression was analyzed by microarray. Differentially expressed 
      genes were validated by qPCR and Western blot. Expression of let-7f was studied 
      by qPCR after inhibition of C/EBPbeta with betulinic acid (BA) and pCMVbeta plasmid and 
      after overexpression of C/EBPbeta with pCMVbeta+ plasmid. ChIP assay was performed to 
      confirm binding of C/EBPbeta to let-7f promoter. Levels of Lin28A/B were checked by 
      qPCR after similar treatment. RESULTS: let-7f knockdown (KD) affects the 
      expression of many transcription factors (eg, C/EBPbeta) which are important 
      regulators of immune responses. We observed let-7f-1 promoter to contain 6 C/EBPbeta 
      binding sites. KD of C/EBPbeta led to decreased let-7f expression while 
      overexpression of C/EBPbeta increased its expression. Treatment of End1/E6E7 cells 
      with TLR-3 ligand, poly(I:C) increased binding of C/EBPbeta at binding sites 3, 5, 
      and 6. Expression of Lin28A/B also changed upon inhibition and overexpression of 
      C/EBPbeta. Its expression is opposite to that of let-7f in End1/E6E7 cells. 
      CONCLUSION: let-7f-1 is a direct target of transcription factor, C/EBPbeta in 
      End1/E6E7 cells.
CI  - (c) 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Ayyar, Kanchana
AU  - Ayyar K
AD  - Division of Molecular Immunology and Microbiology (MIM), National Institute for 
      Research in Reproductive Health (NIRRH), Mumbai, India.
FAU - Reddy, Kudumula Venkata Rami
AU  - Reddy KVR
AUID- ORCID: 0000-0003-0735-7662
AD  - Division of Molecular Immunology and Microbiology (MIM), National Institute for 
      Research in Reproductive Health (NIRRH), Mumbai, India.
LA  - eng
SI  - GENBANK/NM_000600
SI  - GENBANK/NM_004364.4
SI  - GENBANK/NM_005194.3
SI  - GENBANK/NM_172390.2
SI  - GENBANK/NR_003286.2
PT  - Journal Article
DEP - 20170916
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (MicroRNAs)
RN  - 0 (Pentacyclic Triterpenes)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (TLR3 protein, human)
RN  - 0 (Toll-Like Receptor 3)
RN  - 0 (Triterpenes)
RN  - 0 (mirnlet7 microRNA, human)
RN  - O84C90HH2L (Poly I-C)
RN  - 4G6A18707N (Betulinic Acid)
SB  - IM
MH  - CCAAT-Enhancer-Binding Protein-beta/*genetics/metabolism
MH  - Cell Line
MH  - Cervix Uteri/cytology
MH  - Epithelial Cells/*physiology
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Immunity/genetics
MH  - Immunity, Innate
MH  - MicroRNAs/*genetics
MH  - Pentacyclic Triterpenes
MH  - Poly I-C/immunology
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Small Interfering/genetics
MH  - Toll-Like Receptor 3/metabolism
MH  - Triterpenes/pharmacology
MH  - Betulinic Acid
OTO - NOTNLM
OT  - let-7f
OT  - C/EBP
OT  - ChIP
OT  - endocervical (End1/E6E7) cell
OT  - miRNA
OT  - transcription factor
EDAT- 2017/09/19 06:00
MHDA- 2018/06/15 06:00
CRDT- 2017/09/19 06:00
PHST- 2017/03/20 00:00 [received]
PHST- 2017/08/15 00:00 [accepted]
PHST- 2017/09/19 06:00 [pubmed]
PHST- 2018/06/15 06:00 [medline]
PHST- 2017/09/19 06:00 [entrez]
AID - 10.1111/aji.12759 [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2017 Dec;78(6). doi: 10.1111/aji.12759. Epub 2017 Sep 16.