PMID- 28921810
OWN - NLM
STAT- MEDLINE
DCOM- 20190110
LR  - 20190110
IS  - 1097-0029 (Electronic)
IS  - 1059-910X (Linking)
VI  - 80
IP  - 11
DP  - 2017 Nov
TI  - Bones Morphogenic Protein-4 and retinoic acid combined treatment comparative 
      analysis for in vitro differentiation potential of murine mesenchymal stem cells 
      derived from bone marrow and adipose tissue into germ cells.
PG  - 1151-1160
LID - 10.1002/jemt.22880 [doi]
AB  - Nowadays, infertility is no longer considered as an unsolvable disorder due to 
      progresses in germ cells derived from stem lineage with diverse origins. 
      Technical and ethical challenges push researchers to investigate various tissue 
      sources to approach more efficient gametes. The purpose of the current study is 
      to investigate the efficacy of a combined medium, retinoic acid (RA) together 
      with Bone Morphogenic Protein-4 (BMP4), on differentiation of Bone Marrow 
      Mesenchymal Stem Cells (BMMSCs) and adipose-derived mesenchymal stem cells 
      (ADMSCs) into germ cells. Murine MSCs were obtained from both Bone Marrow (BM) 
      and Adipose Tissue (AT) samples and were analyzed for surface markers to get 
      further verification of their nature. BMMSCs and ADMSCs were induced into 
      osteogenic and adipogenic lineage cells respectively, to examine their 
      multipotency. They were finally differentiated into germ cells using media 
      enriched with BMP4 for 4 days followed by addition of RA for 7 days (11 days in 
      total). Analyzing of differentiation potential of BMMSCs- and ADMSCs were 
      performed via Immunofluorescence, Flowcytometry and Real time-PCR techniques for 
      germ cell-specific markers (Mvh, Dazl, Stra8 and Scp3). Mesenchymal surface 
      markers (CD90 and CD44) were expressed on both BMMSCs and ADMSCs, while 
      endothelial and hematopoietic cell markers (CD31 and CD45) had no expression. 
      Finally, all germ-specific markers were expressed in both BM and AT. Although 
      germ cells differentiated from ADMSCs showed faster growth and proliferation as 
      well as easy collection, they significantly expressed germ-specific markers lower 
      than BMMSCs. This suggests stronger differentiation potential of murine BMMSCs 
      than ADMSCs.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Shirzeyli, Maryam H
AU  - Shirzeyli MH
AD  - Department of Biology and Anatomical Sciences, School of Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran.
FAU - Khanlarkhani, Neda
AU  - Khanlarkhani N
AUID- ORCID: 0000-0002-8438-0623
AD  - Department of Anatomical Sciences, Tehran University of Medical Sciences, Tehran, 
      Iran.
FAU - Amidi, Fardin
AU  - Amidi F
AD  - Department of Anatomical Sciences, Tehran University of Medical Sciences, Tehran, 
      Iran.
FAU - Shirzeyli, Farshad H
AU  - Shirzeyli FH
AD  - Department of Animal Science, Science and Research Branch, Islamic Azad 
      University, Tehran, Iran.
FAU - Aval, Fereydoon S
AU  - Aval FS
AD  - Department of Anatomical Sciences, Zahedan University of Medical Sciences, 
      Zahedan, Iran.
FAU - Sobhani, Aligholi
AU  - Sobhani A
AD  - Department of Anatomical Sciences, Tehran University of Medical Sciences, Tehran, 
      Iran.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20170918
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Culture Media)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Adipose Tissue/cytology/physiology
MH  - Animals
MH  - Bone Marrow Cells/cytology/physiology
MH  - Bone Morphogenetic Protein 4/*pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Culture Media/chemistry/pharmacology
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Germ Cells/drug effects/*physiology
MH  - Immunophenotyping
MH  - Infertility/therapy
MH  - Male
MH  - Mesenchymal Stem Cells/*drug effects/physiology
MH  - Mice
MH  - Real-Time Polymerase Chain Reaction
MH  - Signal Transduction/drug effects
MH  - Tretinoin/*pharmacology
OTO - NOTNLM
OT  - differentiation
OT  - germ cell
OT  - infertility
OT  - stem cell
EDAT- 2017/09/19 06:00
MHDA- 2019/01/11 06:00
CRDT- 2017/09/19 06:00
PHST- 2016/11/14 00:00 [received]
PHST- 2017/01/16 00:00 [revised]
PHST- 2017/03/29 00:00 [accepted]
PHST- 2017/09/19 06:00 [pubmed]
PHST- 2019/01/11 06:00 [medline]
PHST- 2017/09/19 06:00 [entrez]
AID - 10.1002/jemt.22880 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2017 Nov;80(11):1151-1160. doi: 10.1002/jemt.22880. Epub 2017 
      Sep 18.