PMID- 28924090
OWN - NLM
STAT- MEDLINE
DCOM- 20180619
LR  - 20181113
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 79
IP  - 11
DP  - 2017 Nov 20
TI  - Porcine alveolar macrophage polarization is involved in inhibition of porcine 
      reproductive and respiratory syndrome virus (PRRSV) replication.
PG  - 1906-1915
LID - 10.1292/jvms.17-0258 [doi]
AB  - Macrophage polarization is a process by which macrophages acquire a distinct 
      phenotypic and functional profile in response to microenvironmental signals. The 
      classically and alternatively activated (M1 and M2, respectively) macrophage 
      phenotypes are defined by the specific molecular characteristics induced in 
      response to prototypic pro- and anti-inflammatory cues. In this study, we used 
      LPS/IFN-gamma and IL-4 to stimulate porcine alveolar macrophages (PAMs) in vitro and 
      investigated the expression changes of several novel markers during macrophage 
      polarization. Notably, we found that LPS/IFN-gamma-stimulated PAMs express 
      prototypical M1 molecules, whereas IL-4-stimulated PAMs express M2 molecules. We 
      also demonstrated that replication of the highly pathogenic porcine reproductive 
      and respiratory syndrome virus (PRRSV) strain HuN4 was effectively suppressed in 
      LPS/IFN-gamma-stimulated M1 PAMs (M1 type), but not IL-4 stimulated M2 PAMs. However, 
      this was not observed with the classic, less pathogenic CH-1a strain. Moreover, 
      we found that M2 marker expression gradually increased after PAM infection with 
      PRRSV, whereas no significant changes were found with M1 marker expression, 
      suggesting that PRRSV infection may skew macrophage polarization towards an M2 
      phenotype. Finally, we found that anti-viral cytokine expression was 
      significantly higher in M1 macrophages than in M2 macrophages or nonpolarized 
      controls. In summary, our results show that PRRSV replication was significantly 
      impaired in M1 PAMs, which may serve as a foundation for further understanding of 
      the dynamic phenotypic changes during macrophage polarization and their effects 
      on viral infection.
FAU - Wang, Longtao
AU  - Wang L
AD  - College of Animal Science and Technology, Jilin Agricultural University, 
      Changchun 130118, China.
FAU - Hu, Shouping
AU  - Hu S
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Liu, Qiang
AU  - Liu Q
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Li, Yiru
AU  - Li Y
AD  - College of Animal Science and Technology, Jilin Agricultural University, 
      Changchun 130118, China.
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Xu, Lu
AU  - Xu L
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
AD  - Department of Animal Medicine, Agricultural College of Yanbian University, Yanji 
      133002, China.
FAU - Zhang, Zhuo
AU  - Zhang Z
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Cai, Xuehui
AU  - Cai X
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - He, Xijun
AU  - He X
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
LA  - eng
PT  - Journal Article
DEP - 20170917
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophage Activation
MH  - Macrophages, Alveolar/cytology/*physiology
MH  - Phenotype
MH  - Porcine respiratory and reproductive syndrome virus/pathogenicity/*physiology
MH  - Swine
MH  - Virus Replication
PMC - PMC5709573
OTO - NOTNLM
OT  - M1/M2 polarization
OT  - porcine alveolar macrophages
OT  - porcine reproductive and respiratory syndrome virus
OT  - viral replication
EDAT- 2017/09/20 06:00
MHDA- 2018/06/21 06:00
PMCR- 2017/11/01
CRDT- 2017/09/20 06:00
PHST- 2017/09/20 06:00 [pubmed]
PHST- 2018/06/21 06:00 [medline]
PHST- 2017/09/20 06:00 [entrez]
PHST- 2017/11/01 00:00 [pmc-release]
AID - 17-0258 [pii]
AID - 10.1292/jvms.17-0258 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2017 Nov 20;79(11):1906-1915. doi: 10.1292/jvms.17-0258. Epub 2017 
      Sep 17.