PMID- 28924549
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2211-3835 (Print)
IS  - 2211-3843 (Electronic)
IS  - 2211-3835 (Linking)
VI  - 7
IP  - 5
DP  - 2017 Sep
TI  - Potassium 2-(1-hydroxypentyl)-benzoate attenuates neuronal apoptosis in 
      neuron-astrocyte co-culture system through neurotrophy and neuroinflammation 
      pathway.
PG  - 554-563
LID - 10.1016/j.apsb.2017.06.006 [doi]
AB  - Potassium 2-(1-hydroxypentyl)-benzoate (d,l-PHPB), a new drug candidate for 
      ischemic stroke at the phase II clinic trial, has been shown to protect neurons 
      by inhibiting oxidative injury and reducing neuron apoptosis in previous studies. 
      But the mechanisms of d,l-PHPB remain to be studied. In this study, a 
      neuron-astrocytes co-culture system was used to elucidate the roles of astrocytes 
      in neuroprotection of d,l-PHPB under oxygen-glucose deprivation/reoxygenation 
      (OGD/R) condition. Our data showed that d,l-PHPB reduced neuronal apoptosis in 
      mono-culture system and this effect was enhanced in neuron-astrocyte co-culture 
      system under the OGD/R condition. Meanwhile, d,l-PHPB obviously increased the 
      levels of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), 
      which were mainly secreted from astrocytes, in the co-culture system after OGD/R. 
      The PI3K/AKT and ERK signaling pathways as well as the p-TRKA/B receptors were 
      involved in the process. In addition, the levels of TNF-alpha and IL-1beta secreted from 
      astrocytes after OGD/R were markedly reduced after d,l-PHPB treatment, which was 
      mainly due to the suppression of phosphorylated p38. In conclusion, the present 
      study demonstrates that the neuroprotective effects of d,l-PHPB were improved by 
      astrocytes, mainly mediated by increasing the release of BDNF/NGF and attenuating 
      inflammatory cytokines.
FAU - Liu, Dongmei
AU  - Liu D
AD  - State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, 
      Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union 
      Medical College, Beijing 100050, China.
FAU - Zhang, Man
AU  - Zhang M
AD  - State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, 
      Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union 
      Medical College, Beijing 100050, China.
FAU - Rong, Xianfang
AU  - Rong X
AD  - State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, 
      Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union 
      Medical College, Beijing 100050, China.
FAU - Li, Jiang
AU  - Li J
AD  - State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, 
      Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union 
      Medical College, Beijing 100050, China.
FAU - Wang, Xiaoliang
AU  - Wang X
AD  - State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, 
      Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union 
      Medical College, Beijing 100050, China.
LA  - eng
PT  - Journal Article
DEP - 20170715
PL  - Netherlands
TA  - Acta Pharm Sin B
JT  - Acta pharmaceutica Sinica. B
JID - 101600560
PMC - PMC5595293
OTO - NOTNLM
OT  - Astrocytes
OT  - Neuro--astrocyte co-culture
OT  - Neuron apoptosis
OT  - Oxygen-glucose deprivation
OT  - d,l-PHPB
EDAT- 2017/09/20 06:00
MHDA- 2017/09/20 06:01
PMCR- 2017/07/15
CRDT- 2017/09/20 06:00
PHST- 2017/05/14 00:00 [received]
PHST- 2017/06/11 00:00 [revised]
PHST- 2017/06/12 00:00 [accepted]
PHST- 2017/09/20 06:00 [entrez]
PHST- 2017/09/20 06:00 [pubmed]
PHST- 2017/09/20 06:01 [medline]
PHST- 2017/07/15 00:00 [pmc-release]
AID - S2211-3835(17)30200-9 [pii]
AID - 10.1016/j.apsb.2017.06.006 [doi]
PST - ppublish
SO  - Acta Pharm Sin B. 2017 Sep;7(5):554-563. doi: 10.1016/j.apsb.2017.06.006. Epub 
      2017 Jul 15.