PMID- 28928022 OWN - NLM STAT- MEDLINE DCOM- 20181106 LR - 20181106 IS - 1090-2430 (Electronic) IS - 0014-4886 (Linking) VI - 301 IP - Pt B DP - 2018 Mar TI - The role of jab1, a putative downstream effector of the neurotrophic cytokine macrophage migration inhibitory factor (MIF) in zebrafish inner ear hair cell development. PG - 100-109 LID - S0014-4886(17)30239-X [pii] LID - 10.1016/j.expneurol.2017.09.009 [doi] AB - Macrophage migration inhibitory factor (MIF) is a neurotrophic cytokine essential for inner ear hair cell (HC) development and statoacoustic ganglion (SAG) neurite outgrowth, and SAG survival in mouse, chick and zebrafish. Another neurotrophic cytokine, Monocyte chemoattractant protein 1 (MCP1) is known to synergize with MIF; but MCP1 alone is insufficient to support mouse/chick SAG neurite outgrowth or neuronal survival. Because of the relatively short time over which the zebrafish inner ear develops (~30hpf), the living zebrafish embryo is an ideal system to examine mif and mcp1 cytokine pathways and interactions. We used a novel technique: direct delivery of antisense oligonucleotide morpholinos (MOs) into the embryonic zebrafish otocyst to discover downstream effectors of mif as well as to clarify the relationship between mif and mcp1 in inner ear development. MOs for mif, mcp1 and the presumptive mif and mcp1 effector, c-Jun activation domain-binding protein-1 (jab1), were injected and then electroporated into the zebrafish otocyst 25-48hours post fertilization (hpf). We found that although mif is important at early stages (before 30hpf) for auditory macular HC development, jab1 is more critical for vestibular macular HC development before 30hpf. After 30hpf, mcp1 becomes important for HC development in both maculae. CI - Copyright (c) 2017 Elsevier Inc. All rights reserved. FAU - Weber, Loren J AU - Weber LJ AD - Department of Cell and Developmental Biology, University of Michigan Medical School, 3728 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA. Electronic address: weberlo1@msu.edu. FAU - Marcy, Hannah K AU - Marcy HK AD - Department of Cell and Developmental Biology, University of Michigan Medical School, 3728 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA; Undergraduate Research Opportunity Program, 1190 Undergraduate Science Building, 204 Washtenaw Avenue, Ann Arbor, MI 48109-2215, USA. Electronic address: hkmarcy@wustl.edu. FAU - Shen, Yu-Chi AU - Shen YC AD - Department of Cell and Developmental Biology, University of Michigan Medical School, 3728 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA. Electronic address: yshen@umich.edu. FAU - Tomkovich, Sarah E AU - Tomkovich SE AD - Department of Cell and Developmental Biology, University of Michigan Medical School, 3728 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA; Undergraduate Research Opportunity Program, 1190 Undergraduate Science Building, 204 Washtenaw Avenue, Ann Arbor, MI 48109-2215, USA. Electronic address: Sarah.Tomkovich@medicine.ufl.edu. FAU - Brooks, Kristina M AU - Brooks KM AD - Department of Cell and Developmental Biology, University of Michigan Medical School, 3728 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA. Electronic address: kristina.brooks@nih.gov. FAU - Hilk, Kelly E AU - Hilk KE AD - Department of Cell and Developmental Biology, University of Michigan Medical School, 3728 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA; Undergraduate Research Opportunity Program, 1190 Undergraduate Science Building, 204 Washtenaw Avenue, Ann Arbor, MI 48109-2215, USA. Electronic address: kelly.hilk@med.wayne.edu. FAU - Barald, Kate F AU - Barald KF AD - Department of Cell and Developmental Biology, University of Michigan Medical School, 3728 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA; Cellular and Molecular Biology Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109-0619, USA; Department of Biomedical Engineering, College of Engineering, 2200 Bonisteel Boulevard, University of Michigan, Ann Arbor, MI 48109-2099, USA. Electronic address: kfbarald@umich.edu. LA - eng PT - Journal Article DEP - 20170918 PL - United States TA - Exp Neurol JT - Experimental neurology JID - 0370712 RN - 0 (Actins) RN - 0 (Chemokine CCL2) RN - 0 (Cytokines) RN - 0 (Macrophage Migration-Inhibitory Factors) RN - 0 (Oligonucleotides, Antisense) RN - 0 (Tubulin) RN - EC 3.4.19.12 (COP9 Signalosome Complex) SB - IM MH - Acoustic Maculae/embryology/growth & development MH - Actins/metabolism MH - Animals MH - Axons/drug effects MH - COP9 Signalosome Complex/genetics/*physiology MH - Chemokine CCL2/metabolism MH - Cytokines/biosynthesis MH - Embryo, Nonmammalian MH - Hair Cells, Auditory, Inner/*physiology MH - Macrophage Migration-Inhibitory Factors/genetics/*physiology MH - Oligonucleotides, Antisense/pharmacology MH - Oocysts/growth & development MH - Saccule and Utricle/embryology/growth & development MH - Tubulin/metabolism MH - Zebrafish OTO - NOTNLM OT - Hair cell development OT - Inner ear OT - Mif OT - Neurotrophic cytokines OT - Statoacoustic ganglion OT - VIII nerve OT - Zebrafish OT - jab1 OT - mcp1 EDAT- 2017/09/21 06:00 MHDA- 2018/11/07 06:00 CRDT- 2017/09/21 06:00 PHST- 2017/05/02 00:00 [received] PHST- 2017/09/05 00:00 [revised] PHST- 2017/09/12 00:00 [accepted] PHST- 2017/09/21 06:00 [pubmed] PHST- 2018/11/07 06:00 [medline] PHST- 2017/09/21 06:00 [entrez] AID - S0014-4886(17)30239-X [pii] AID - 10.1016/j.expneurol.2017.09.009 [doi] PST - ppublish SO - Exp Neurol. 2018 Mar;301(Pt B):100-109. doi: 10.1016/j.expneurol.2017.09.009. Epub 2017 Sep 18.