PMID- 28928819
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 14
IP  - 3
DP  - 2017 Sep
TI  - Curcumin induced autophagy anticancer effects on human lung adenocarcinoma cell 
      line A549.
PG  - 2775-2782
LID - 10.3892/ol.2017.6565 [doi]
AB  - To investigate the anticancer effects of curcumin-induced autophagy and its 
      effects on the human lung adenocarcinoma A549 cell line, inverted phase contrast 
      microscopy was used to observe alterations to the cytomorphology of cells. An MTT 
      assay was used to measure cell viability. Autophagy was detected using acridine 
      orange (AO) staining and 3-methyladenine (3-MA) was used as an autophagy-specific 
      inhibitor. Dose- and time-dependent A549 cell viability inhibition was observed 
      following curcumin treatment. A dose-dependent increase in the red fluorescent 
      structures in A549 cells was identified following curcumin treatment for 48 h 
      through AO staining. In addition, the activation of autophagy was determined 
      through changes in the number of autophagic vesicles (AVs; fluorescent particles) 
      infected with monodansylcadaverine (MDC). The fluorescence intensity and density 
      of AVs in the curcumin-treated groups were higher at 48 h compared with the 
      control group. Finally, the MTT assay demonstrated that the survival rates of the 
      curcumin-treated cells were increased when pretreated with 3-MA for 3 h, 
      indicating that the inhibitory effect of curcumin on A549 cells is reduced 
      following the inhibition of autophagy. Furthermore, AO and MDC staining confirmed 
      that 3-MA does inhibit the induction of autophagy. Thus, it was hypothesized that 
      the induction of autophagy is partially involved in the reduction of cell 
      viability observed following curcumin treatment. The anticancer effects of 
      curcumin on A549 cells can be reduced using autophagy inhibitors. This suggests a 
      possible cancer therapeutic application of curcumin through the activation of 
      autophagy. These findings have improved the understanding of the mechanism 
      underlying the anticancer property of curcumin.
FAU - Liu, Furong
AU  - Liu F
AD  - Department of Public Health, Medical College of Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710061, P.R. China.
FAU - Gao, Song
AU  - Gao S
AD  - Department of Public Health, Medical College of Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710061, P.R. China.
FAU - Yang, Yuxuan
AU  - Yang Y
AD  - Department of Public Health, Medical College of Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710061, P.R. China.
FAU - Zhao, Xiaodan
AU  - Zhao X
AD  - Department of Public Health, Medical College of Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710061, P.R. China.
FAU - Fan, Yameng
AU  - Fan Y
AD  - Department of Public Health, Medical College of Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710061, P.R. China.
FAU - Ma, Wenxia
AU  - Ma W
AD  - Department of Public Health, Medical College of Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710061, P.R. China.
FAU - Yang, Danrong
AU  - Yang D
AD  - Department of Public Health, Medical College of Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710061, P.R. China.
FAU - Yang, Aimin
AU  - Yang A
AD  - Department of Nuclear Medicine, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, P.R. China.
FAU - Yu, Yan
AU  - Yu Y
AD  - Department of Public Health, Medical College of Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710061, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170708
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5588543
OTO - NOTNLM
OT  - 3-MA
OT  - A549 cell
OT  - anticancer
OT  - autophagy
OT  - curcumin
EDAT- 2017/09/21 06:00
MHDA- 2017/09/21 06:01
PMCR- 2017/07/08
CRDT- 2017/09/21 06:00
PHST- 2017/02/16 00:00 [received]
PHST- 2017/06/28 00:00 [accepted]
PHST- 2017/09/21 06:00 [entrez]
PHST- 2017/09/21 06:00 [pubmed]
PHST- 2017/09/21 06:01 [medline]
PHST- 2017/07/08 00:00 [pmc-release]
AID - OL-0-0-6565 [pii]
AID - 10.3892/ol.2017.6565 [doi]
PST - ppublish
SO  - Oncol Lett. 2017 Sep;14(3):2775-2782. doi: 10.3892/ol.2017.6565. Epub 2017 Jul 8.