PMID- 28929987
OWN - NLM
STAT- MEDLINE
DCOM- 20180612
LR  - 20190816
IS  - 1469-8730 (Electronic)
IS  - 0967-1994 (Linking)
VI  - 25
IP  - 5
DP  - 2017 Oct
TI  - Cell and genetic predictors of human blastocyst hatching success in assisted 
      reproduction.
PG  - 631-636
LID - 10.1017/S0967199417000508 [doi]
AB  - The aim was to identify cell and genetic predictors of human blastocyst hatching 
      success in assisted reproduction programmes via a prospective case-control study. 
      Blastocysts, donated by couples in assisted reproduction programmes were used. 
      Hatching success assessment was performed after 144-146 h post-fertilization. The 
      mRNA expression levels of cathepsin V (CTSV), GATA-binding protein 3 (GATA3) and 
      human chorionic gonadotropin beta subunit 3, 5, 7 and 8 (CGB) genes were detected 
      by quantitative real-time polymerase chain reaction. The odds ratio (OR) of 
      hatching due to zona pellucida (ZP) thickness, oocyte and sperm quality, embryo 
      quality and mRNA expression of CTSV, GATA3 and CGB genes in blastocysts was 
      determined. From 62 blastocysts included in the study, 47 (75.8%) were unable to 
      hatch spontaneously. The ZP thickening, and oocyte and sperm quality did not 
      affect human blastocyst ability to hatch, except the combination of cytoplasmic 
      and extracytoplasmic oocyte dysmorphisms (OR = 1.25; 95% confidence interval = 
      1.08, 1.45). Hatching-capable blastocysts had higher Gardner scale grade and mRNA 
      expression of CTSV, GATA3 and CGB genes than hatching-incapable blastocysts. The 
      human blastocyst hatching success depends on the blastocyst Gardner grade, but 
      not on ZP and gamete quality. Blastocyst development was regulated by CTSV, GATA3 
      and CGB gene expression.
FAU - Syrkasheva, Anastasiya G
AU  - Syrkasheva AG
AD  - Research Center for Obstetrics,Gynecology and Perinatology,4 Oparin 
      str,117997,Moscow,Russia.
FAU - Dolgushina, Nataliya V
AU  - Dolgushina NV
AD  - Research Center for Obstetrics,Gynecology and Perinatology,4 Oparin 
      str,117997,Moscow,Russia.
FAU - Romanov, Andrey Yu
AU  - Romanov AY
AD  - Research Center for Obstetrics,Gynecology and Perinatology,4 Oparin 
      str,117997,Moscow,Russia.
FAU - Burmenskaya, Olga V
AU  - Burmenskaya OV
AD  - Research Center for Obstetrics,Gynecology and Perinatology,4 Oparin 
      str,117997,Moscow,Russia.
FAU - Makarova, Nataliya P
AU  - Makarova NP
AD  - Research Center for Obstetrics,Gynecology and Perinatology,4 Oparin 
      str,117997,Moscow,Russia.
FAU - Ibragimova, Espet O
AU  - Ibragimova EO
AD  - Research Center for Obstetrics,Gynecology and Perinatology,4 Oparin 
      str,117997,Moscow,Russia.
FAU - Kalinina, Elena A
AU  - Kalinina EA
AD  - Research Center for Obstetrics,Gynecology and Perinatology,4 Oparin 
      str,117997,Moscow,Russia.
FAU - Sukhikh, Gennady T
AU  - Sukhikh GT
AD  - Research Center for Obstetrics,Gynecology and Perinatology,4 Oparin 
      str,117997,Moscow,Russia.
LA  - eng
PT  - Journal Article
DEP - 20170920
PL  - England
TA  - Zygote
JT  - Zygote (Cambridge, England)
JID - 9309124
RN  - 0 (Chorionic Gonadotropin, beta Subunit, Human)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (GATA3 protein, human)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.43 (CTSV protein, human)
SB  - IM
MH  - Blastocyst/cytology/*metabolism
MH  - Case-Control Studies
MH  - Cathepsins/genetics
MH  - Chorionic Gonadotropin, beta Subunit, Human/genetics
MH  - Cysteine Endopeptidases/genetics
MH  - Embryonic Development/*genetics
MH  - Female
MH  - Fertilization in Vitro/*methods
MH  - GATA3 Transcription Factor/genetics
MH  - *Gene Expression Regulation, Developmental
MH  - Humans
MH  - Prospective Studies
MH  - Zona Pellucida/metabolism
OTO - NOTNLM
OT  - CGB
OT  - CTSV
OT  - GATA3
OT  - Assisted reproduction
OT  - Blastocyst
OT  - Hatching
EDAT- 2017/09/21 06:00
MHDA- 2018/06/13 06:00
CRDT- 2017/09/21 06:00
PHST- 2017/09/21 06:00 [pubmed]
PHST- 2018/06/13 06:00 [medline]
PHST- 2017/09/21 06:00 [entrez]
AID - S0967199417000508 [pii]
AID - 10.1017/S0967199417000508 [doi]
PST - ppublish
SO  - Zygote. 2017 Oct;25(5):631-636. doi: 10.1017/S0967199417000508. Epub 2017 Sep 20.