PMID- 28932888
OWN - NLM
STAT- MEDLINE
DCOM- 20180625
LR  - 20221207
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 101
IP  - 20
DP  - 2017 Oct
TI  - Assessment of molecular detection of anaerobic ammonium-oxidizing (anammox) 
      bacteria in different environmental samples using PCR primers based on 16S rRNA 
      and functional genes.
PG  - 7689-7702
LID - 10.1007/s00253-017-8502-3 [doi]
AB  - Eleven published PCR primer sets for detecting genes encoding 16S ribosomal RNA 
      (rRNA), hydrazine oxidoreductase (HZO), cytochrome cd (1)-containing nitrite 
      reductase (NirS), and hydrazine synthase subunit A (HzsA) of anaerobic 
      ammonium-oxidizing (anammox) bacteria were assessed for the diversity and 
      abundance of anammox bacteria in samples of three environments: wastewater 
      treatment plant (WWTP), wetland of Mai Po Nature Reserve (MP), and the South 
      China Sea (SCS). Consistent phylogenetic results of three biomarkers (16S rRNA, 
      hzo, and hzsA) of anammox bacteria were obtained from all samples. WWTP had the 
      lowest diversity with Candidatus Kuenenia dominating while the SCS was dominated 
      by Candidatus Scalindua. MP showed the highest diversity of anammox bacteria 
      including C. Scalindua, C. Kuenenia, and Candidatus Brocadia. Comparing different 
      primer sets, no significant differences in specificity for 16S rRNA gene could be 
      distinguished. Primer set CL1 showed relatively high efficiency in detecting the 
      anammox bacterium hzo gene from all samples, while CL2 showed greater selectivity 
      for WWTP samples. The recently reported primer sets of the hzsA gene resulted in 
      high efficiencies in detecting anammox bacteria while nirS primer sets were more 
      selective for specific samples. Results collectively indicate that the 
      distribution of anammox bacteria is niche-specific within different ecosystems 
      and primer specificity may cause biases on the diversity detected.
FAU - Han, Ping
AU  - Han P
AD  - Laboratory of Environmental Microbiology and Toxicology, School of Biological 
      Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, People's 
      Republic of China.
FAU - Klumper, Uli
AU  - Klumper U
AD  - Laboratory of Environmental Microbiology and Toxicology, School of Biological 
      Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, People's 
      Republic of China.
AD  - Department of Chemistry, University of Duisburg-Essen, Duisburg, Germany.
FAU - Wong, Alex
AU  - Wong A
AD  - Laboratory of Environmental Microbiology and Toxicology, School of Biological 
      Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, People's 
      Republic of China.
FAU - Li, Meng
AU  - Li M
AD  - The Institute for Advanced Study, Shenzhen University, Shenzhen, People's 
      Republic of China.
FAU - Lin, Jih-Gaw
AU  - Lin JG
AD  - Institute of Environmental Engineering, National Chiao Tung University, 1001, 
      University Road, Hsinchu, 30010, Taiwan.
FAU - Quan, Zhexue
AU  - Quan Z
AD  - Department of Microbiology and Microbial Engineering, School of Life Sciences, 
      Fudan University, Shanghai, 200433, People's Republic of China.
FAU - Denecke, Martin
AU  - Denecke M
AD  - Department of Urban Water and Waste Management, University of Duisburg-Essen, 
      Universitatsstrasse 15, 45141, Essen, Germany.
FAU - Gu, Ji-Dong
AU  - Gu JD
AUID- ORCID: 0000-0002-7082-9784
AD  - Laboratory of Environmental Microbiology and Toxicology, School of Biological 
      Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, People's 
      Republic of China. jdgu@hku.hk.
AD  - State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee 
      Avenue, Kowloon, Hong Kong SAR, People's Republic of China. jdgu@hku.hk.
LA  - eng
GR  - 701913/Hong Kong Research Grant Council GRF Project/
PT  - Evaluation Study
PT  - Journal Article
DEP - 20170920
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Ammonium Compounds)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Waste Water)
SB  - IM
MH  - Ammonium Compounds/*metabolism
MH  - Bacteria, Anaerobic/*genetics/*isolation & purification/metabolism
MH  - Bacteriological Techniques/*methods
MH  - China
MH  - DNA Primers/genetics
MH  - DNA, Bacterial/genetics
MH  - *Environmental Microbiology
MH  - Genes, Bacterial
MH  - Oxidation-Reduction
MH  - Polymerase Chain Reaction/*methods
MH  - RNA, Ribosomal, 16S/genetics
MH  - Wastewater/*microbiology
OTO - NOTNLM
OT  - 16S rRNA
OT  - Anammox bacteria
OT  - Diversity
OT  - Molecular detection
OT  - Nitrogen cycle
OT  - hzo
OT  - hzsA
OT  - nirS
EDAT- 2017/09/22 06:00
MHDA- 2018/06/26 06:00
CRDT- 2017/09/22 06:00
PHST- 2017/05/24 00:00 [received]
PHST- 2017/08/26 00:00 [accepted]
PHST- 2017/08/10 00:00 [revised]
PHST- 2017/09/22 06:00 [pubmed]
PHST- 2018/06/26 06:00 [medline]
PHST- 2017/09/22 06:00 [entrez]
AID - 10.1007/s00253-017-8502-3 [pii]
AID - 10.1007/s00253-017-8502-3 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2017 Oct;101(20):7689-7702. doi: 
      10.1007/s00253-017-8502-3. Epub 2017 Sep 20.