PMID- 28933046
OWN - NLM
STAT- MEDLINE
DCOM- 20190531
LR  - 20211005
IS  - 1874-270X (Electronic)
IS  - 1874-2718 (Print)
IS  - 1874-270X (Linking)
VI  - 12
IP  - 1
DP  - 2018 Apr
TI  - NMR backbone resonance assignments of the prodomain variants of BDNF in the urea 
      denatured state.
PG  - 43-45
LID - 10.1007/s12104-017-9777-0 [doi]
AB  - Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family 
      of proteins which plays a central role in neuronal survival, growth, plasticity 
      and memory. A single Val66Met variant has been identified in the prodomain of 
      human BDNF that is associated with anxiety, depression and memory disorders. The 
      structural differences within the full-length prodomain Val66 and Met66 isoforms 
      could shed light on the mechanism of action of the Met66 and its impact on the 
      development of neuropsychiatric-associated disorders. In the present study, we 
      report the backbone (1)H, (13)C, and (15)N NMR assignments of both full-length 
      Val66 and Met66 prodomains in the presence of 2 M urea. These conditions were 
      utilized to suppress residual structure and aid subsequent native state 
      structural investigations aimed at mapping and identifying variant-dependent 
      conformational differences under native-state conditions.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Biochemistry, Weill Cornell Medical College, 1300 York Avenue, Box 
      63, New York, NY, 10065, USA.
FAU - Bains, Henrietta
AU  - Bains H
AD  - Department of Biochemistry, Weill Cornell Medical College, 1300 York Avenue, Box 
      63, New York, NY, 10065, USA.
FAU - Anastasia, Agustin
AU  - Anastasia A
AD  - Instituto Ferreyra (INIMEC-CONICET-Universidad Nacional de Cordoba), Cordoba, 
      Argentina.
FAU - Bracken, Clay
AU  - Bracken C
AUID- ORCID: 0000-0002-8067-7796
AD  - Department of Biochemistry, Weill Cornell Medical College, 1300 York Avenue, Box 
      63, New York, NY, 10065, USA. wcb2001@med.cornell.edu.
LA  - eng
GR  - NS090322/NS/NINDS NIH HHS/United States
GR  - S10 RR023694/RR/NCRR NIH HHS/United States
GR  - RR023694/RR/NCRR NIH HHS/United States
GR  - R21 NS090322/NS/NINDS NIH HHS/United States
GR  - S10 OD016320/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20170920
PL  - Netherlands
TA  - Biomol NMR Assign
JT  - Biomolecular NMR assignments
JID - 101472371
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 8W8T17847W (Urea)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*chemistry
MH  - Humans
MH  - *Nuclear Magnetic Resonance, Biomolecular
MH  - Protein Denaturation/*drug effects
MH  - Protein Domains
MH  - Urea/*pharmacology
PMC - PMC6865803
MID - NIHMS1003902
OTO - NOTNLM
OT  - BDNF
OT  - Intrinsically disordered proteins
OT  - Neurotrophin
OT  - Prodomain
OT  - Urea
OT  - Val66Met
EDAT- 2017/09/22 06:00
MHDA- 2019/06/01 06:00
PMCR- 2019/11/20
CRDT- 2017/09/22 06:00
PHST- 2017/06/07 00:00 [received]
PHST- 2017/09/13 00:00 [accepted]
PHST- 2017/09/22 06:00 [pubmed]
PHST- 2019/06/01 06:00 [medline]
PHST- 2017/09/22 06:00 [entrez]
PHST- 2019/11/20 00:00 [pmc-release]
AID - 10.1007/s12104-017-9777-0 [pii]
AID - 10.1007/s12104-017-9777-0 [doi]
PST - ppublish
SO  - Biomol NMR Assign. 2018 Apr;12(1):43-45. doi: 10.1007/s12104-017-9777-0. Epub 
      2017 Sep 20.