PMID- 28933054 OWN - NLM STAT- MEDLINE DCOM- 20180730 LR - 20180730 IS - 1534-4681 (Electronic) IS - 1068-9265 (Linking) VI - 24 IP - 13 DP - 2017 Dec TI - High STMN1 Expression is Associated with Cancer Progression and Chemo-Resistance in Lung Squamous Cell Carcinoma. PG - 4017-4024 LID - 10.1245/s10434-017-6083-0 [doi] AB - BACKGROUND: Known as a microtubule-destabilizing protein, STMN1 (gene symbol: STMN1) regulates the dynamics of microtubules, cell cycle progress, and chemo-resistance against taxane agents. It is highly expressed in various human cancers and involved in cancer progression as well as poor prognosis. METHODS: Expression of STMN1 was examined by immunohistochemistry using FFPE tissue sections from 186 patients with lung squamous cell carcinoma (LSCC). Analysis of STMN1 suppression was performed for STMN1 small interfering RNA (siRNA)-transfected LSCC cell lines to determine the change in proliferation, invasive and apoptosis abilities, and paclitaxel sensitivity. RESULTS: The cytoplasmic STMN1 expression in LSCC was higher than in normal tissues. The high expression was significantly associated with vascular invasion (P = 0.0477) and poor prognosis. In addition, the proliferating and invasive abilities were decreased, and the apoptosis ability and paclitaxel sensitivity were increased in STMN1-suppressed LSCC cells compared with control cells. CONCLUSION: The results suggest that STMN1 is a prognostic factor that also is associated with caner progression and chemo-resistance. Therefore, STMN1 could be a predictor for poor prognosis and a potential therapeutic target in LSCC. FAU - Bao, Pinjie AU - Bao P AD - Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan. FAU - Yokobori, Takehiko AU - Yokobori T AD - Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan. bori45@gunma-u.ac.jp. AD - Research Program for Omics-Based Medical Science, Division of Integrated Oncology Research, Gunma University Initiative for Advanced Research, Gunma, Japan. bori45@gunma-u.ac.jp. FAU - Altan, Bolag AU - Altan B AD - Department of Oncology Clinical Development, Graduate School of Medicine, Gunma University, Gunma, Japan. FAU - Iijima, Misaki AU - Iijima M AD - Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan. FAU - Azuma, Youko AU - Azuma Y AD - Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan. FAU - Onozato, Ryoichi AU - Onozato R AD - Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan. FAU - Yajima, Toshiki AU - Yajima T AD - Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan. FAU - Watanabe, Akira AU - Watanabe A AD - Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan. FAU - Mogi, Akira AU - Mogi A AD - Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan. FAU - Shimizu, Kimihiro AU - Shimizu K AD - Department of Thoracic and Visceral Organ Surgery, Graduate School of Medicine, Gunma University, Gunma, Japan. FAU - Nagashima, Toshiteru AU - Nagashima T AD - Department of Thoracic and Visceral Organ Surgery, Graduate School of Medicine, Gunma University, Gunma, Japan. FAU - Ohtaki, Yoichi AU - Ohtaki Y AD - Department of Thoracic and Visceral Organ Surgery, Graduate School of Medicine, Gunma University, Gunma, Japan. FAU - Obayashi, Kai AU - Obayashi K AD - Department of Thoracic and Visceral Organ Surgery, Graduate School of Medicine, Gunma University, Gunma, Japan. FAU - Nakazawa, Seshiru AU - Nakazawa S AD - Department of Thoracic and Visceral Organ Surgery, Graduate School of Medicine, Gunma University, Gunma, Japan. FAU - Bai, Tuya AU - Bai T AD - Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan. FAU - Kawabata-Iwakawa, Reika AU - Kawabata-Iwakawa R AD - Department of Molecular Pharmacology and Oncology, Graduate School of Medicine, Gunma University, Gunma, Japan. FAU - Asao, Takayuki AU - Asao T AD - Big Data Center for Integrative Analysis, Gunma University Initiative for Advance Research, Gunma, Japan. FAU - Kaira, Kyoichi AU - Kaira K AD - Department of Oncology Clinical Development, Graduate School of Medicine, Gunma University, Gunma, Japan. FAU - Nishiyama, Masahiko AU - Nishiyama M AD - Department of Molecular Pharmacology and Oncology, Graduate School of Medicine, Gunma University, Gunma, Japan. FAU - Kuwano, Hiroyuki AU - Kuwano H AD - Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan. LA - eng PT - Journal Article DEP - 20170920 PL - United States TA - Ann Surg Oncol JT - Annals of surgical oncology JID - 9420840 RN - 0 (Biomarkers, Tumor) RN - 0 (RNA, Small Interfering) RN - 0 (STMN1 protein, human) RN - 0 (Stathmin) RN - P88XT4IS4D (Paclitaxel) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Apoptosis MH - Biomarkers, Tumor/*metabolism MH - Carcinoma, Squamous Cell/drug therapy/metabolism/*secondary MH - Cell Line, Tumor MH - Cell Movement MH - Cell Proliferation MH - *Drug Resistance, Neoplasm MH - Female MH - Follow-Up Studies MH - *Gene Expression Regulation, Neoplastic MH - Humans MH - Lung Neoplasms/drug therapy/metabolism/*pathology MH - Lymphatic Metastasis MH - Male MH - Middle Aged MH - Neoplasm Invasiveness MH - Paclitaxel/pharmacology MH - Prognosis MH - RNA, Small Interfering MH - Stathmin/antagonists & inhibitors/genetics/*metabolism MH - Survival Rate EDAT- 2017/09/22 06:00 MHDA- 2018/07/31 06:00 CRDT- 2017/09/22 06:00 PHST- 2017/01/23 00:00 [received] PHST- 2017/09/22 06:00 [pubmed] PHST- 2018/07/31 06:00 [medline] PHST- 2017/09/22 06:00 [entrez] AID - 10.1245/s10434-017-6083-0 [pii] AID - 10.1245/s10434-017-6083-0 [doi] PST - ppublish SO - Ann Surg Oncol. 2017 Dec;24(13):4017-4024. doi: 10.1245/s10434-017-6083-0. Epub 2017 Sep 20.