PMID- 28933095 OWN - NLM STAT- MEDLINE DCOM- 20180530 LR - 20181202 IS - 1001-5302 (Print) IS - 1001-5302 (Linking) VI - 41 IP - 22 DP - 2016 Nov TI - [Investigation of effects of albiflorin and paeoniflorin on hippocampal BDNF and NO in chronic restraint stress rats]. PG - 4240-4246 LID - 10.4268/cjcmm20162225 [doi] AB - This paper aimed to investigate the antidepressant-like effects and the mechanism of the albiflorin, paeoniflorin on rats with chronic restraint stress model. Fluoxetine and Xiaoyao group served as the positive control, body weight, sucrose preference test and the open field behavioral experiment were measured, the levels of nitric oxide (NO) in hippocampus were detected by Greg colorimetric method. Furthermore, the levels of brain derived neurotrophic factor (BDNF) in hippocampus were detected by ELISA. Finally, the expressions of nNOS mRNA in hippocampus detected by RT-qPCR, the protein levels of nNOS and BDNF in hippocampus were detected by Western blot. Compared with the model group,the pass counts of paeoniflorin group(30 mg*kg(-)(1)) and albiflorin group(30 mg*kg(-)(1)) were obviously increased(P<0.05,P<0.01). Furthermore, compared with the model group,the levels of NO in paeoniflorin groups(30 mg*kg(-)(1) and 15 mg*kg(-)(1)) and albiflorin groups(30 mg*kg(-)(1) and 15 mg*kg(-)(1)) were all significantly decreased(P<0.05,P<0.01; P<0.05, P<0.05). BDNF levels of paeoniflorin group(30 mg*kg(-)(1)) and albiflorin group(30 mg*kg(-)(1)) were obviously increased(P<0.05). Finally, compared with the model group, the expressions of nNOS mRNA of paeoniflorin groups(30 mg*kg(-)(1) and 15 mg*kg(-)(1)) (P<0.01, P<0.01) and albiflorin groups(30 mg*kg(-)(1)) were significantly decreased(P<0.05). Compared with the model group, the protein exprsssions of BDNF of paeoniflorin group(30 mg*kg(-)(1)) and albiflorin group(30 mg*kg(-)(1)) were obviously increased(P<0.05). Albiflorin and paeoniflorin have the effects of smooth the liver and dispel stagnation, the mechanism has the relevant with adjusting and controlling the expression of NO and BDNF in hippocampus. CI - Copyright(c) by the Chinese Pharmaceutical Association. FAU - Zhu, Ying-Li AU - Zhu YL AD - Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Wang, Lin-Yuan AU - Wang LY AD - Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Wang, Cheng-Long AU - Wang CL AD - Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Zhao, Dan-Ping AU - Zhao DP AD - Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Wang, Sha AU - Wang S AD - Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Fei, Wen-Ting AU - Fei WT AD - Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Zhang, Jian-Jun AU - Zhang JJ AD - Beijing University of Chinese Medicine, Beijing 100029, China. LA - chi PT - Journal Article PL - China TA - Zhongguo Zhong Yao Za Zhi JT - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JID - 8913656 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Bridged-Ring Compounds) RN - 0 (Glucosides) RN - 0 (Monoterpenes) RN - 21AIQ4EV64 (peoniflorin) RN - 31C4KY9ESH (Nitric Oxide) RN - 39011-90-0 (albiflorin) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Bridged-Ring Compounds/*pharmacology MH - Glucosides/*pharmacology MH - Hippocampus/drug effects/*metabolism MH - Monoterpenes/*pharmacology MH - Nitric Oxide/*metabolism MH - Rats MH - Rats, Sprague-Dawley MH - *Restraint, Physical MH - Stress, Physiological OTO - NOTNLM OT - albiflorin OT - brain derived neurotrophic factor OT - liver stagnation syndrome OT - nitric oxide OT - paeoniflorin COIS- The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose. EDAT- 2017/09/22 06:00 MHDA- 2018/05/31 06:00 CRDT- 2017/09/22 06:00 PHST- 2016/07/19 00:00 [received] PHST- 2017/09/22 06:00 [entrez] PHST- 2017/09/22 06:00 [pubmed] PHST- 2018/05/31 06:00 [medline] AID - 10.4268/cjcmm20162225 [doi] PST - ppublish SO - Zhongguo Zhong Yao Za Zhi. 2016 Nov;41(22):4240-4246. doi: 10.4268/cjcmm20162225.