PMID- 28934984
OWN - NLM
STAT- MEDLINE
DCOM- 20171206
LR  - 20181113
IS  - 0717-6287 (Electronic)
IS  - 0716-9760 (Print)
IS  - 0716-9760 (Linking)
VI  - 50
IP  - 1
DP  - 2017 Sep 21
TI  - A simple and rapid identification method for newly emerged porcine 
      Deltacoronavirus with loop-mediated isothermal amplification.
PG  - 30
LID - 10.1186/s40659-017-0135-6 [doi]
LID - 30
AB  - BACKGROUND: Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic 
      coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has 
      spread to many countries around the world, leading to significant economic losses 
      in the pork industry. Therefore, a rapid and sensitive method for detection of 
      PDCoV in clinical samples is urgently needed. RESULTS: In this study, we 
      developed a single-tube one-step reverse transcription loop-mediated isothermal 
      amplification (RT-LAMP) assay specific for nucleocapsid gene to diagnose and 
      monitor PDCoV infections. The detection limit of RT-LAMP assay was 1 x 10(1) 
      copies of PDCoV, which was approximately 100-fold more sensitive than gel-based 
      one-step reverse transcription polymerase chain reaction (RT-PCR). This assay 
      could specifically amplify PDCoV and had no cross amplification with porcine 
      epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), 
      porcine kobuvirus (PKoV), porcine astrovirus (PAstV), porcine reproductive and 
      respiratory syndrome virus (PRRSV), classic swine fever virus (CSFV), and porcine 
      circovirus type 2 (PCV2). By screening a panel of clinical specimens (N = 192), 
      this method presented a similar sensitivity with nested RT-PCR and was 1-2 log 
      more sensitive than conventional RT-PCR in detection of PDCoV. CONCLUSIONS: The 
      RT-LAMP assay established in this study is a potentially valuable tool, 
      especially in low-resource laboratories and filed settings, for a rapid 
      diagnosis, surveillance, and molecular epidemiology investigation of PDCoV 
      infections. To the best of our knowledge, this is the first work for detection of 
      newly emerged PDCoV with LAMP technology.
FAU - Zhang, Fanfan
AU  - Zhang F
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Ye, Yu
AU  - Ye Y
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Song, Deping
AU  - Song D
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Guo, Nannan
AU  - Guo N
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Peng, Qi
AU  - Peng Q
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Li, Anqi
AU  - Li A
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Zhou, Xingrong
AU  - Zhou X
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Chen, Yanjun
AU  - Chen Y
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Zhang, Min
AU  - Zhang M
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Huang, Dongyan
AU  - Huang D
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Tang, Yuxin
AU  - Tang Y
AD  - Department of Veterinary Preventive Medicine, College of Animal Science and 
      Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China. 
      tang53ster@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20170921
PL  - England
TA  - Biol Res
JT  - Biological research
JID - 9308271
SB  - IM
MH  - Animals
MH  - Coronaviridae/*isolation & purification
MH  - Coronavirus Infections/diagnosis/veterinary/*virology
MH  - Nucleic Acid Amplification Techniques/veterinary
MH  - Polymerase Chain Reaction/veterinary
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/diagnosis/*virology
PMC - PMC5607838
OTO - NOTNLM
OT  - Porcine Deltacoronavirus (PDCoV)
OT  - RT-LAMP
OT  - Rapid diagnosis
EDAT- 2017/09/25 06:00
MHDA- 2017/12/07 06:00
PMCR- 2017/09/21
CRDT- 2017/09/23 06:00
PHST- 2016/10/26 00:00 [received]
PHST- 2017/08/08 00:00 [accepted]
PHST- 2017/09/23 06:00 [entrez]
PHST- 2017/09/25 06:00 [pubmed]
PHST- 2017/12/07 06:00 [medline]
PHST- 2017/09/21 00:00 [pmc-release]
AID - 10.1186/s40659-017-0135-6 [pii]
AID - 135 [pii]
AID - 10.1186/s40659-017-0135-6 [doi]
PST - epublish
SO  - Biol Res. 2017 Sep 21;50(1):30. doi: 10.1186/s40659-017-0135-6.