PMID- 28938486
OWN - NLM
STAT- MEDLINE
DCOM- 20171212
LR  - 20181202
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Linking)
VI  - 158
IP  - 11
DP  - 2017 Nov 1
TI  - The Two Populations of Kisspeptin Neurons Are Involved in the Ram-Induced LH 
      Pulsatile Secretion and LH Surge in Anestrous Ewes.
PG  - 3914-3928
LID - 10.1210/en.2017-00429 [doi]
AB  - Exposure to a ram during spring stimulates luteinizing hormone (LH) secretion and 
      can induce ovulation in sexually quiescent ewes ("ram effect"). Kisspeptin (Kiss) 
      present in the arcuate nucleus (ARC) and the preoptic area (POA) is a potent 
      stimulators of LH secretion. Our aim was to investigate whether Kiss neurons 
      mediate the increase in LH secretion during the ram effect. With double 
      immunofluorescent detection, we identified Kiss neurons (Kiss IR) activated (Fos 
      IR) by exposure to a ram for 2 hours (M2) or 12 hours (M12) or to ewes for 2 
      hours (C). The density of cells Kiss + Fos IR and the proportion of Kiss IR cells 
      that were also Fos IR cells were higher in M2 and M12 than in C in ARC (P < 
      0.002) and POA (P < 0.02). In ARC, these parameters were also higher in M12 than 
      in M2 (P < 0.02 and P < 0.05). Kiss antagonist (P234 10-6M) administered by 
      retrodialysis in POA for 3 hours at the time of introduction of the ram reduced 
      the amplitude of the male-induced increase in LH concentration compared with 
      solvent (P < 0.02). In ARC, P234 had a more limited effect (P < 0.038 1 hour 
      after P234) but pulse frequency increased less than after solvent (P = 0.07). In 
      contrast, Kiss antagonist (P271 10-4M) infused in ARC but not POA 6 to 18 hours 
      after introduction of the ram prevented the LH surge in the ewe (0/6 vs 4/5 and 
      4/6 in C). These results suggest that both populations of Kiss neurons are 
      involved in the ram-induced pulsatile LH secretion and in the LH surge.
CI  - Copyright (c) 2017 Endocrine Society.
FAU - Fabre-Nys, Claude
AU  - Fabre-Nys C
AD  - Unite Mixte de Recherche 7247 Physiologie de la Reproduction et des 
      Comportements, Centre National de la Recherche Scientifique, Institut National de 
      Recherche Agronomique (INRA), University of Tours, Institut Francais du Cheval et 
      de l'Equitation, Institut Federatif de Recherche 135, 37380 Nouzilly, France.
FAU - Cognie, Juliette
AU  - Cognie J
AD  - Unite Mixte de Recherche 7247 Physiologie de la Reproduction et des 
      Comportements, Centre National de la Recherche Scientifique, Institut National de 
      Recherche Agronomique (INRA), University of Tours, Institut Francais du Cheval et 
      de l'Equitation, Institut Federatif de Recherche 135, 37380 Nouzilly, France.
FAU - Dufourny, Laurence
AU  - Dufourny L
AD  - Unite Mixte de Recherche 7247 Physiologie de la Reproduction et des 
      Comportements, Centre National de la Recherche Scientifique, Institut National de 
      Recherche Agronomique (INRA), University of Tours, Institut Francais du Cheval et 
      de l'Equitation, Institut Federatif de Recherche 135, 37380 Nouzilly, France.
FAU - Ghenim, Meriem
AU  - Ghenim M
AD  - Unite Mixte de Recherche 7247 Physiologie de la Reproduction et des 
      Comportements, Centre National de la Recherche Scientifique, Institut National de 
      Recherche Agronomique (INRA), University of Tours, Institut Francais du Cheval et 
      de l'Equitation, Institut Federatif de Recherche 135, 37380 Nouzilly, France.
FAU - Martinet, Stephanie
AU  - Martinet S
AD  - Unite Mixte de Recherche 7247 Physiologie de la Reproduction et des 
      Comportements, Centre National de la Recherche Scientifique, Institut National de 
      Recherche Agronomique (INRA), University of Tours, Institut Francais du Cheval et 
      de l'Equitation, Institut Federatif de Recherche 135, 37380 Nouzilly, France.
FAU - Lasserre, Olivier
AU  - Lasserre O
AD  - INRA Unite Experimentale de Physiologie Animale de l'Orfrasiere, 37380 Nouzilly, 
      France.
FAU - Lomet, Didier
AU  - Lomet D
AD  - Unite Mixte de Recherche 7247 Physiologie de la Reproduction et des 
      Comportements, Centre National de la Recherche Scientifique, Institut National de 
      Recherche Agronomique (INRA), University of Tours, Institut Francais du Cheval et 
      de l'Equitation, Institut Federatif de Recherche 135, 37380 Nouzilly, France.
FAU - Millar, Robert P
AU  - Millar RP
AD  - Centre for Neuroendocrinology, Department of Physiology, University of Pretoria, 
      Pretoria 0084, South Africa.
AD  - Mammal Research Institute, Department of Zoology and Entomology, Institute of 
      Infectious Diseases, University of Cape Town, Cape Town 7925, South Africa.
FAU - Ohkura, Satoshi
AU  - Ohkura S
AD  - Nagoya University, Nagoya 464-8601, Japan.
FAU - Suetomi, Yuta
AU  - Suetomi Y
AD  - Nagoya University, Nagoya 464-8601, Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Kisspeptins)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Anestrus/blood/*metabolism
MH  - Animal Husbandry
MH  - Animals
MH  - Female
MH  - Kisspeptins/*metabolism
MH  - Luteinizing Hormone/*metabolism
MH  - Male
MH  - Neurons/cytology/*metabolism
MH  - Physical Stimulation
MH  - Sexual Behavior, Animal/*physiology
MH  - Sheep/*physiology
EDAT- 2017/09/25 06:00
MHDA- 2017/12/13 06:00
CRDT- 2017/09/23 06:00
PHST- 2017/05/02 00:00 [received]
PHST- 2017/08/29 00:00 [accepted]
PHST- 2017/09/25 06:00 [pubmed]
PHST- 2017/12/13 06:00 [medline]
PHST- 2017/09/23 06:00 [entrez]
AID - 4102061 [pii]
AID - 10.1210/en.2017-00429 [doi]
PST - ppublish
SO  - Endocrinology. 2017 Nov 1;158(11):3914-3928. doi: 10.1210/en.2017-00429.