PMID- 28939080
OWN - NLM
STAT- MEDLINE
DCOM- 20180614
LR  - 20180708
IS  - 1879-0593 (Electronic)
IS  - 1368-8375 (Linking)
VI  - 73
DP  - 2017 Oct
TI  - Prevalence of high-risk human papillomavirus infection and cancer gene mutations 
      in nonmalignant tonsils.
PG  - 77-82
LID - S1368-8375(17)30244-0 [pii]
LID - 10.1016/j.oraloncology.2017.08.010 [doi]
AB  - OBJECTIVES: To analyze the prevalence of high-risk HPV (human papillomavirus) and 
      genetic alterations in nonmalignant tonsils. METHODS: We collected benign fresh 
      tonsillar tissue specimens from 477 patients undergoing tonsillectomy because of 
      chronic tonsillitis or tonsillar hypertrophy in 2012 (Group A, n=237) and in 2015 
      (Group B, n=240). Luminex xMAP technique served to detect E6/E7 DNA from 16 
      different high-risk HPV types. Tonsillar DNA and peripheral blood leukocyte DNA 
      from the infected individuals were analyzed using Nimblegen SeqCap EZ 
      Comprehensive Cancer Design panel. The panel targets 578 different genes that are 
      relevant in carcinogenesis. HPV negative tonsillar specimens from age- and gender 
      matched individuals were used as controls. All specimens harboring high-risk HPV 
      were analyzed using fluorescence in situ hybridization (FISH). RESULTS: Five of 
      477 (1.0%) patients tested positive for the following HPV types: HPV16 (two 
      cases), HPV52 (one case), HPV66 (one case), HPV52 and HPV68 (coinfection, one 
      case). FISH analyses showed that the appearance of HPV in specimens infected with 
      HPV 16 was episomal. Benign tonsils infected with high-risk HPV harbored 
      mutations in EP300, NF1, PIK3CA, and RB1 which are considered relevant in the 
      development of HPV-associated head and neck squamous cell carcinoma (SCC). 
      CONCLUSIONS: The prevalence of high-risk HPV in nonmalignant tonsils is low. 
      High-risk HPV positive tonsils harbored mutations in genes that are commonly 
      altered in HPV-associated head and neck SCC. The role of these mutations in 
      tonsillar carcinogenesis is an interesting target for future research.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Ilmarinen, Taru
AU  - Ilmarinen T
AD  - Department of Otorhinolaryngology - Head and Neck Surgery, University of Helsinki 
      and Helsinki University Hospital, P.O. Box 263, 00130 Helsinki, Finland. 
      Electronic address: taru.t.ilmarinen@hus.fi.
FAU - Munne, Pauliina
AU  - Munne P
AD  - Institute for Molecular Medicine Finland (FIMM), University of Helsinki, P.O. Box 
      20, 00014 Helsinki, Finland.
FAU - Hagstrom, Jaana
AU  - Hagstrom J
AD  - Department of Pathology, University of Helsinki and Helsinki University Hospital, 
      P.O. Box 21, 00014 Helsinki, Finland.
FAU - Haglund, Caj
AU  - Haglund C
AD  - Department of Surgery and Pathology, University of Helsinki and Helsinki 
      University Hospital, P.O. Box 21, 00014 Helsinki, Finland; Research Programs 
      Unit, Translational Cancer Biology, University of Helsinki, P.O. Box 21, 00014 
      Helsinki, Finland.
FAU - Auvinen, Eeva
AU  - Auvinen E
AD  - Department of Virology, University of Helsinki and Helsinki University Hospital 
      Laboratory, P.O. Box 21, 00014 Helsinki, Finland.
FAU - Virtanen, Elina I
AU  - Virtanen EI
AD  - Department of Virology, University of Helsinki and Helsinki University Hospital 
      Laboratory, P.O. Box 21, 00014 Helsinki, Finland.
FAU - Haesevoets, Annick
AU  - Haesevoets A
AD  - Department of Pathology, GROW-School for Oncology & Developmental Biology, 
      Maastricht University Medical Center, P.O. Box 5800, 6202 AZ Maastricht, The 
      Netherlands.
FAU - Speel, Ernst J M
AU  - Speel EJM
AD  - Department of Pathology, GROW-School for Oncology & Developmental Biology, 
      Maastricht University Medical Center, P.O. Box 5800, 6202 AZ Maastricht, The 
      Netherlands.
FAU - Aaltonen, Leena-Maija
AU  - Aaltonen LM
AD  - Department of Otorhinolaryngology - Head and Neck Surgery, University of Helsinki 
      and Helsinki University Hospital, P.O. Box 263, 00130 Helsinki, Finland.
LA  - eng
PT  - Journal Article
DEP - 20170818
PL  - England
TA  - Oral Oncol
JT  - Oral oncology
JID - 9709118
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Human papillomavirus 16/isolation & purification
MH  - Humans
MH  - Infant
MH  - Male
MH  - Middle Aged
MH  - *Mutation
MH  - Palatine Tonsil/*metabolism
MH  - Prevalence
MH  - Risk Factors
MH  - Tonsillar Neoplasms/genetics/virology
MH  - Young Adult
OTO - NOTNLM
OT  - Cancer gene
OT  - Human papillomavirus
OT  - Mutation
OT  - Squamous cell carcinoma
OT  - Tonsil
EDAT- 2017/09/25 06:00
MHDA- 2018/06/15 06:00
CRDT- 2017/09/24 06:00
PHST- 2017/04/15 00:00 [received]
PHST- 2017/08/11 00:00 [revised]
PHST- 2017/08/14 00:00 [accepted]
PHST- 2017/09/24 06:00 [entrez]
PHST- 2017/09/25 06:00 [pubmed]
PHST- 2018/06/15 06:00 [medline]
AID - S1368-8375(17)30244-0 [pii]
AID - 10.1016/j.oraloncology.2017.08.010 [doi]
PST - ppublish
SO  - Oral Oncol. 2017 Oct;73:77-82. doi: 10.1016/j.oraloncology.2017.08.010. Epub 2017 
      Aug 18.