PMID- 28941404
OWN - NLM
STAT- MEDLINE
DCOM- 20171030
LR  - 20171030
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1065-1066
DP  - 2017 Oct 15
TI  - Development of a simple multi-residue determination method of 80 veterinary drugs 
      in Oplegnathus punctatus by liquid chromatography coupled to quadrupole Orbitrap 
      mass spectrometry.
PG  - 20-28
LID - S1570-0232(17)31272-2 [pii]
LID - 10.1016/j.jchromb.2017.09.013 [doi]
AB  - A simple, rapid and sensitive multi-residue analytical method was developed and 
      validated for 80 veterinary drugs in Oplegnathus punctatus using ultrahigh 
      performance liquid chromatography-Orbitrap high resolution mass spectrometry 
      (LC-HRMS). The analytes belong to 12 different families include benzimidazoles, 
      beta-lactams, lincosamides, macrolides, nitromidazoles, quinolones, sulfonamides and 
      trimethoprim, tetracyclines, triphenylmethane dyes, amphenicols, nonsteroidal 
      estrogens and steroid hormones. The sample preparation was optimized base on 
      QuEChERS (quick, easy, cheap, effective, rugged and safe) procedure. A very 
      simple and sufficient preparation procedure without salting-out and complex 
      clean-up process was studied. It had been proved that water in the extract was 
      helpful for extracting hydrophilic compounds and precipitating the lipids during 
      the subsequent cleaning process. In addition, an appropriate percent of methanol 
      was necessary to some analytes. Finally, a mixture of acetonitrile, methanol and 
      water (3:1:1, v/v/v) which include 1% acetic acid and 10mM 
      ethylenediaminetetraacetic acid disodium salt 2-hydrate was selected as the 
      extraction solvent, and the clean-up step consisted of a low temperature 
      procedure and two times of high-speed centrifugation to deproteinize and remove 
      lipids. The detection and quantification of all compounds were performed by 
      ultrahigh performance liquid chromatography coupled with electrospray ionization 
      quadrupole Orbitrap high resolution mass spectrometry in positive and negative 
      ion mode. This methodology was validated according to the Commission Decision 
      2002/657/EC and SANTE/11945/2015. The recoveries ranged from 60.74%-109.85% with 
      relative standard deviations (RSDs)<20%. The limits of quantification (LOQs) were 
      0.25-25ug/kg, for the analytes which the MRL or MRPL had been established in fish 
      tissue, the LOQs were all lower than their own legal tolerances. The values of 
      decision limit (CCalpha) and detection capability (CCbeta) were in the range of 
      1.91-1001.13ug/kg and 3.52-1002.26ug/kg, respectively. This validated method has 
      been successfully applied on the determination of veterinary drugs in real 
      commercial oplegnathus punctatus samples.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Zhao, Fei
AU  - Zhao F
AD  - College of Food Science and Engineering, Ocean University of China, 5 Yushan 
      Road, Qingdao 266003, China; Shandong Entry-Exit Inspection and Quarantine 
      Bureau, 70 Qutangxia Road, Qingdao 266002, China.
FAU - Gao, Xin
AU  - Gao X
AD  - College of Food Science and Engineering, Ocean University of China, 5 Yushan 
      Road, Qingdao 266003, China. Electronic address: xingao@ouc.edu.cn.
FAU - Tang, Zhixu
AU  - Tang Z
AD  - Shandong Entry-Exit Inspection and Quarantine Bureau, 70 Qutangxia Road, Qingdao 
      266002, China. Electronic address: zhixutang@126.com.
FAU - Luo, Xin
AU  - Luo X
AD  - Shandong Entry-Exit Inspection and Quarantine Bureau, 70 Qutangxia Road, Qingdao 
      266002, China.
FAU - Wu, Miaomiao
AU  - Wu M
AD  - College of Food Science and Engineering, Ocean University of China, 5 Yushan 
      Road, Qingdao 266003, China.
FAU - Xu, Jiachao
AU  - Xu J
AD  - College of Food Science and Engineering, Ocean University of China, 5 Yushan 
      Road, Qingdao 266003, China.
FAU - Fu, Xiaoting
AU  - Fu X
AD  - College of Food Science and Engineering, Ocean University of China, 5 Yushan 
      Road, Qingdao 266003, China.
LA  - eng
PT  - Journal Article
DEP - 20170918
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Veterinary Drugs)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - *Fishes
MH  - Limit of Detection
MH  - Linear Models
MH  - Mass Spectrometry/*methods
MH  - Reproducibility of Results
MH  - Seafood/*analysis
MH  - Veterinary Drugs/*analysis/isolation & purification
OTO - NOTNLM
OT  - Extraction optimization
OT  - Fish tissues
OT  - Multi-residue
OT  - Orbitrap
OT  - Veterinary drugs
EDAT- 2017/09/25 06:00
MHDA- 2017/10/31 06:00
CRDT- 2017/09/24 06:00
PHST- 2017/07/24 00:00 [received]
PHST- 2017/09/01 00:00 [revised]
PHST- 2017/09/07 00:00 [accepted]
PHST- 2017/09/25 06:00 [pubmed]
PHST- 2017/10/31 06:00 [medline]
PHST- 2017/09/24 06:00 [entrez]
AID - S1570-0232(17)31272-2 [pii]
AID - 10.1016/j.jchromb.2017.09.013 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Oct 15;1065-1066:20-28. doi: 
      10.1016/j.jchromb.2017.09.013. Epub 2017 Sep 18.