PMID- 28942210
OWN - NLM
STAT- MEDLINE
DCOM- 20180619
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 100
DP  - 2018 Feb 15
TI  - High sensitivity cardiac troponin I detection in physiological environment using 
      AlGaN/GaN High Electron Mobility Transistor (HEMT) Biosensors.
PG  - 282-289
LID - S0956-5663(17)30624-3 [pii]
LID - 10.1016/j.bios.2017.09.018 [doi]
AB  - In this study, we report the development of a high sensitivity assay for the 
      detection of cardiac troponin I using electrical double layer gated high field 
      AlGaN/GaN HEMT biosensor. The unique gating mechanism overcomes the drawback of 
      charge screening seen in traditional FET based biosensors, allowing detection of 
      target proteins in physiological solutions without sample processing steps. 
      Troponin I specific antibody and aptamer are used as receptors. The tests carried 
      out using purified protein solution and clinical serum samples depict high 
      sensitivity, specificity and wide dynamic range (0.006-148ng/mL). No additional 
      wash or sample pre-treatment steps are required, which greatly simplifies the 
      biosensor system. The miniaturized HEMT chip is packaged in a polymer substrate 
      and easily integrated with a portable measurement unit, to carry out quantitative 
      troponin I detection in serum samples with < 2microl sample volume in 5min. The 
      integrated prototype biosensor unit demonstrates the potential of the method as a 
      rapid, inexpensive, high sensitivity CVD biomarker assay. The highly simplified 
      protocols and enhanced sensor performance make our biosensor an ideal choice for 
      point of care diagnostics and personal healthcare systems.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Sarangadharan, Indu
AU  - Sarangadharan I
AD  - Institute of NanoEngineering and Microsystems, National Tsing Hua University, 
      Hsinchu 300, Taiwan R.O.C.
FAU - Regmi, Abiral
AU  - Regmi A
AD  - Institute of NanoEngineering and Microsystems, National Tsing Hua University, 
      Hsinchu 300, Taiwan R.O.C.
FAU - Chen, Yen-Wen
AU  - Chen YW
AD  - Institute of NanoEngineering and Microsystems, National Tsing Hua University, 
      Hsinchu 300, Taiwan R.O.C.
FAU - Hsu, Chen-Pin
AU  - Hsu CP
AD  - Institute of NanoEngineering and Microsystems, National Tsing Hua University, 
      Hsinchu 300, Taiwan R.O.C.
FAU - Chen, Pei-Chi
AU  - Chen PC
AD  - Institute of NanoEngineering and Microsystems, National Tsing Hua University, 
      Hsinchu 300, Taiwan R.O.C.
FAU - Chang, Wen-Hsin
AU  - Chang WH
AD  - Department of Power Mechanical Engineering, National Tsing Hua University, 
      Hsinchu 300, Taiwan R.O.C.
FAU - Lee, Geng-Yen
AU  - Lee GY
AD  - Department of Electrical Engineering, National Central University, Jhongli City, 
      Taoyuan County 320, Taiwan R.O.C.
FAU - Chyi, Jen-Inn
AU  - Chyi JI
AD  - Department of Electrical Engineering, National Central University, Jhongli City, 
      Taoyuan County 320, Taiwan R.O.C.
FAU - Shiesh, Shu-Chu
AU  - Shiesh SC
AD  - Department of Medical Laboratory Science and Biotechnology, National Cheng-Kung 
      University, Tainan City 701, Taiwan R.O.C.
FAU - Lee, Gwo-Bin
AU  - Lee GB
AD  - Institute of NanoEngineering and Microsystems, National Tsing Hua University, 
      Hsinchu 300, Taiwan R.O.C; Department of Power Mechanical Engineering, National 
      Tsing Hua University, Hsinchu 300, Taiwan R.O.C; Institute of Biomedical 
      Engineering, National Tsing Hua University, Hsinchu 300, Taiwan R.O.C.
FAU - Wang, Yu-Lin
AU  - Wang YL
AD  - Institute of NanoEngineering and Microsystems, National Tsing Hua University, 
      Hsinchu 300, Taiwan R.O.C; Department of Power Mechanical Engineering, National 
      Tsing Hua University, Hsinchu 300, Taiwan R.O.C. Electronic address: 
      ylwang@mx.nthu.edu.tw.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20170914
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Aluminum Compounds)
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Biomarkers)
RN  - 0 (Troponin I)
RN  - 0 (aluminum gallium nitride)
RN  - 1R9CC3P9VL (gallium nitride)
RN  - CH46OC8YV4 (Gallium)
SB  - IM
MH  - Aluminum Compounds/*chemistry
MH  - Antibodies, Immobilized/chemistry
MH  - Biomarkers/analysis/blood
MH  - Biosensing Techniques/*instrumentation/methods
MH  - Electrons
MH  - Equipment Design
MH  - Gallium/*chemistry
MH  - Humans
MH  - Point-of-Care Systems
MH  - Troponin I/analysis/*blood
OTO - NOTNLM
OT  - Electrical double layer gated AlGaN/GaN HEMT biosensor
OT  - High sensitivity cardiac Troponin I detection
OT  - Personal healthcare
OT  - Point of care diagnostics
EDAT- 2017/09/25 06:00
MHDA- 2018/06/21 06:00
CRDT- 2017/09/25 06:00
PHST- 2017/07/02 00:00 [received]
PHST- 2017/09/07 00:00 [revised]
PHST- 2017/09/12 00:00 [accepted]
PHST- 2017/09/25 06:00 [pubmed]
PHST- 2018/06/21 06:00 [medline]
PHST- 2017/09/25 06:00 [entrez]
AID - S0956-5663(17)30624-3 [pii]
AID - 10.1016/j.bios.2017.09.018 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2018 Feb 15;100:282-289. doi: 10.1016/j.bios.2017.09.018. 
      Epub 2017 Sep 14.