PMID- 28942222
OWN - NLM
STAT- MEDLINE
DCOM- 20180612
LR  - 20180612
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 52
DP  - 2017 Nov
TI  - The effect of Astragaloside IV on ethanol-induced gastric mucosal injury in rats: 
      Involvement of inflammation.
PG  - 211-217
LID - S1567-5769(17)30350-8 [pii]
LID - 10.1016/j.intimp.2017.09.011 [doi]
AB  - The present study aimed to investigate the potential protective effects of 
      Astragaloside IV (AS-IV) against ethanol-induced gastric mucosal injury in rats. 
      The animals were divided into 7 groups and pretreated with vehicle, various doses 
      of AS-IV (1,2 and 4mg/kg, i.p.) or omeprazole (40mg/kg), 75min later, the gastric 
      mucosal injury was induced by oral administration of ethanol. One hour after 
      ethanol ingestion, the rats were euthanized and gastric tissues were collected to 
      biochemical analyze. Myeloperoxidase (MPO), tumor necrosis factor alpha (TNF-alpha), 
      interleukin 1beta (IL-1beta), interleukin 10 (IL-10), nuclear factor kappa B (NF-kappaB) 
      p65 protein, TNF receptor-associated factor 2 (TRAF2) and nuclear NF-kappaB (nNF-kappaB) 
      proteins were estimated by enzyme-linked immunosorbent assay or western blot 
      analysis. The gastric mucosal lesions were assessed by macroscopic and 
      histopathological examinations. The results showed pretreatment with AS-IV 
      attenuated the severity of ethanol gastric mucosal damage as evidenced by 
      lowering of injury scores, histopathologic aberrations and leukocyte invasion. 
      These actions were analogous to the reference omeprazole. AS-IV suppressed 
      gastric inflammation by curbing of MPO, TNF-alpha levels along with NF-kappaB p65 and 
      TRAF2 expression. It also augmented the anti-inflammatory IL-10 levels. 
      Meanwhile, AS-IV could inhibit NF-kappaB transcription by inhibiting the expression 
      of NF-kappaB p65 and increasing the expression of nNF-kappaB. It seems that AS-IV as an 
      anti-inflammatory agent may have a protective effect against ethanol-induced 
      mucosal injury by inhibition of neutrophil infiltration and reducing the 
      expression of NF-kappaB p65, TRAF2 and inflammatory cytokines via regulating 
      TNF-alpha/NF-kappaB signal pathway in gastric tissue.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Qin, Shumin
AU  - Qin S
AD  - Department of Gastroenterology, Guangdong Provincial Hospital of Traditional 
      Chinese Medicine, Guangzhou 510120, PR China; Guangzhou University of Chinese 
      Medicine, Guangzhou 510405, PR China.
FAU - Huang, Keer
AU  - Huang K
AD  - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou 510405, PR China. Electronic address: huangkeer@gzucm.edu.cn.
FAU - Fang, Zhigang
AU  - Fang Z
AD  - Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China.
FAU - Yin, Jinjin
AU  - Yin J
AD  - Department of Pharmacy, The Third Affiliated Hospital of Guangzhou Medical 
      University, Guangzhou 510150, PR China.
FAU - Dai, Ruwei
AU  - Dai R
AD  - Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20170921
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (NF-kappa B)
RN  - 0 (Saponins)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (Triterpenes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3A592W8XKE (astragaloside A)
RN  - 3K9958V90M (Ethanol)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - KG60484QX9 (Omeprazole)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*therapeutic use
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Ethanol/toxicity
MH  - Gastritis/chemically induced/*drug therapy
MH  - Humans
MH  - Inflammation Mediators/metabolism
MH  - Intestines/drug effects/immunology/*pathology
MH  - Male
MH  - NF-kappa B/metabolism
MH  - Neutrophils/*immunology
MH  - Omeprazole/therapeutic use
MH  - Peroxidase
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Saponins/*therapeutic use
MH  - TNF Receptor-Associated Factor 2/metabolism
MH  - Triterpenes/*therapeutic use
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Astragaloside IV
OT  - Gastric mucosal injury
OT  - Inflammation
EDAT- 2017/09/25 06:00
MHDA- 2018/06/13 06:00
CRDT- 2017/09/25 06:00
PHST- 2017/01/31 00:00 [received]
PHST- 2017/09/14 00:00 [revised]
PHST- 2017/09/14 00:00 [accepted]
PHST- 2017/09/25 06:00 [pubmed]
PHST- 2018/06/13 06:00 [medline]
PHST- 2017/09/25 06:00 [entrez]
AID - S1567-5769(17)30350-8 [pii]
AID - 10.1016/j.intimp.2017.09.011 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2017 Nov;52:211-217. doi: 10.1016/j.intimp.2017.09.011. Epub 
      2017 Sep 21.