PMID- 28943455
OWN - NLM
STAT- MEDLINE
DCOM- 20180925
LR  - 20191008
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 208
DP  - 2018 Jun
TI  - Assaying uptake of endocrine disruptor compounds in zebrafish embryos and larvae.
PG  - 105-113
LID - S1532-0456(17)30162-X [pii]
LID - 10.1016/j.cbpc.2017.09.007 [doi]
AB  - To study the effects of environmental endocrine disruptor compounds (EDCs) on 
      aquatic animals, embryos and larvae are typically incubated in water containing 
      defined concentrations of EDCs. However, the amount of EDC uptake into the animal 
      is often difficult to determine. Using radiolabeled estradiol ([(3)H]E2), we 
      previously developed a rapid, straightforward assay to measure estradiol uptake 
      from water into zebrafish embryos and larvae. Here, we extend this approach to 
      measure the uptake of two additional EDCs, bisphenol A (BPA) and ethinyl 
      estradiol (EE2). As with E2, the uptake of each compound by individual larvae was 
      low (<6%), and increased with increasing concentration, duration, and 
      developmental stage. We found that E2 and EE2 had similar uptake under equivalent 
      exposure conditions, while BPA had comparatively lower uptake. One application of 
      this assay is to test factors that influence EDC uptake or efflux. It has been 
      suggested that persistent organic pollutants (POPs) inhibit ABC transporters that 
      may normally efflux EDCs and their metabolites, inducing toxicity in aquatic 
      organisms. We measured [(3)H]E2 levels in zebrafish in the presence or absence of 
      the POP PDBE-100, and cyclosporine A, a known inhibitor of ABC transporters. 
      Neither chemical significantly affected [(3)H]E2 levels in zebrafish, suggesting 
      that zebrafish maintain estradiol efflux in the presence of PDBE-100, 
      independently of cyclosporine A-responsive transporters. These uptake results 
      will be a valuable reference for EDC exposure studies in developing zebrafish, 
      and provide a rapid assay to screen for chemicals that influence estrogen-like 
      EDC levels in vivo.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Souder, J Paige
AU  - Souder JP
AD  - University of Alabama at Birmingham, Department of Pharmacology and Toxicology, 
      Birmingham, AL 35294, USA. Electronic address: jsouder@uab.edu.
FAU - Gorelick, Daniel A
AU  - Gorelick DA
AD  - University of Alabama at Birmingham, Department of Pharmacology and Toxicology, 
      Birmingham, AL 35294, USA. Electronic address: danielg@uab.edu.
LA  - eng
GR  - R01 ES026337/ES/NIEHS NIH HHS/United States
GR  - T32 GM008361/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20170921
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Abcb4 protein, zebrafish)
RN  - 0 (Benzhydryl Compounds)
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Phenols)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0 (Zebrafish Proteins)
RN  - 423D2T571U (Ethinyl Estradiol)
RN  - 4TI98Z838E (Estradiol)
RN  - 7REL09ZX35 (pentabromodiphenyl ether)
RN  - 83HN0GTJ6D (Cyclosporine)
RN  - MLT3645I99 (bisphenol A)
SB  - IM
MH  - ATP-Binding Cassette Transporters/antagonists & inhibitors/*metabolism
MH  - Age Factors
MH  - Animals
MH  - Benzhydryl Compounds/metabolism
MH  - Cyclosporine/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Embryo, Nonmammalian/*metabolism
MH  - Endocrine Disruptors/*metabolism/toxicity
MH  - Estradiol/metabolism
MH  - Ethinyl Estradiol/metabolism
MH  - Halogenated Diphenyl Ethers/pharmacology
MH  - Larva/metabolism
MH  - Phenols/metabolism
MH  - Time Factors
MH  - Water Pollutants, Chemical/*metabolism/toxicity
MH  - Zebrafish/embryology/*metabolism
MH  - Zebrafish Proteins/antagonists & inhibitors/*metabolism
PMC - PMC5862746
MID - NIHMS907767
OTO - NOTNLM
OT  - Bisphenol A
OT  - Endocrine disruptors
OT  - Estrogens
OT  - Zebrafish
EDAT- 2017/09/26 06:00
MHDA- 2018/09/27 06:00
PMCR- 2019/06/01
CRDT- 2017/09/26 06:00
PHST- 2017/07/28 00:00 [received]
PHST- 2017/09/18 00:00 [revised]
PHST- 2017/09/20 00:00 [accepted]
PHST- 2017/09/26 06:00 [pubmed]
PHST- 2018/09/27 06:00 [medline]
PHST- 2017/09/26 06:00 [entrez]
PHST- 2019/06/01 00:00 [pmc-release]
AID - S1532-0456(17)30162-X [pii]
AID - 10.1016/j.cbpc.2017.09.007 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2018 Jun;208:105-113. doi: 
      10.1016/j.cbpc.2017.09.007. Epub 2017 Sep 21.