PMID- 28943876
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 8
DP  - 2017
TI  - Dysbiosis of the Urinary Microbiota Associated With Urine Levels of 
      Proinflammatory Chemokine Interleukin-8 in Female Type 2 Diabetic Patients.
PG  - 1032
LID - 10.3389/fimmu.2017.01032 [doi]
LID - 1032
AB  - Evidence has shown that dysbiosis of the urinary microbiota existed in female 
      type 2 diabetes mellitus (T2DM) patients. Perturbations of intestinal microbiota 
      are linked to proinflammatory chemokine interleukin-8 (IL-8); however, the 
      correlations between urinary microbiota and IL-8 are not well studied. Here, we 
      investigated the associations between the altered urinary microbiota and urinary 
      IL-8 in female T2DM patients. A modified four-tube midstream urine technique was 
      used to collect urine specimens from 70 female T2DM patients and 70 matched 
      healthy controls (HCs). Bacterial genomic DNA from urine specimens was isolated 
      using magnetic beads and the urinary microbiota was assessed using Illumina MiSeq 
      platform targeting on the 16S rRNA gene V3-V4 region. Urinary IL-8 was determined 
      by enzyme linked immunosorbent assay. Subsequently, the T2DM patients were 
      separated into urine IL-8 detectable (WIL8) and undetectable (NIL8) groups, and 
      the composition of urinary microbiota between the two groups was compared. 
      Meanwhile, the levels of IL-8 between the ">/=HCs" group (those specific bacterial 
      genera were more than or equal to the HCs) and the "<HCs" group (those specific 
      bacterial genera were less than the HCs) was also compared. Of 70 urine samples 
      from T2DM patients without urinary tract infections, 46 patients had detectable 
      IL-8 in their urine (64.31 +/- 70.43 pg/mL), while 24 patients had undetectable 
      IL-8. Compared to the NIL8 group, 11 bacterial genera increased in the WIL8 
      group, including Corynebacterium, Akkermansia, Enterococcus, etc., whereas 10 
      genera, such as Faecalibacterium, Bacteroides, and Pseudomonas decreased. One 
      species of Lactobacillus, Lactobacillus iners, increased obviously in the WIL8 
      group. The ">/=HCs" group showed 17 genera increased and 16 genera decreased. In 
      addition, 18 genera contributed to the presence of urinary IL-8 in T2DM patients, 
      which explained 95.60% of the total variance of urinary microbiota. Our study 
      demonstrated that dysbiosis of the urinary microbiota with several key bacteria 
      was associated with urinary IL-8 in female T2DM patients, which might be useful 
      to explore the interactions between urinary microbiota and inflammatory responses 
      and shed light on novel diagnosis and therapy for urinary microbiota associated 
      with infections in T2DM patients.
FAU - Ling, Zongxin
AU  - Ling Z
AD  - Collaborative Innovation Center for Diagnosis and Treatment of Infectious 
      Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious 
      Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang 
      University, Hangzhou, China.
FAU - Liu, Fengping
AU  - Liu F
AD  - Collaborative Innovation Center for Diagnosis and Treatment of Infectious 
      Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious 
      Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang 
      University, Hangzhou, China.
AD  - Nursing School, Jiangsu Vocational College of Medicine, Yancheng, China.
FAU - Shao, Li
AU  - Shao L
AD  - Collaborative Innovation Center for Diagnosis and Treatment of Infectious 
      Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious 
      Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang 
      University, Hangzhou, China.
FAU - Cheng, Yiwen
AU  - Cheng Y
AD  - Collaborative Innovation Center for Diagnosis and Treatment of Infectious 
      Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious 
      Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang 
      University, Hangzhou, China.
FAU - Li, Lanjuan
AU  - Li L
AD  - Collaborative Innovation Center for Diagnosis and Treatment of Infectious 
      Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious 
      Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang 
      University, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20170825
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
PMC - PMC5603796
OTO - NOTNLM
OT  - Akkermansia
OT  - Lactobacillus
OT  - interleukin-8
OT  - type 2 diabetes mellitus
OT  - urinary microbiota
EDAT- 2017/09/26 06:00
MHDA- 2017/09/26 06:01
PMCR- 2017/01/01
CRDT- 2017/09/26 06:00
PHST- 2017/05/27 00:00 [received]
PHST- 2017/08/10 00:00 [accepted]
PHST- 2017/09/26 06:00 [entrez]
PHST- 2017/09/26 06:00 [pubmed]
PHST- 2017/09/26 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2017.01032 [doi]
PST - epublish
SO  - Front Immunol. 2017 Aug 25;8:1032. doi: 10.3389/fimmu.2017.01032. eCollection 
      2017.