PMID- 28945272
OWN - NLM
STAT- MEDLINE
DCOM- 20171222
LR  - 20220330
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 191
IP  - 1
DP  - 2018 Jan
TI  - CD4(+) T helper cells and regulatory T cells in active lupus nephritis: an 
      imbalance towards a predominant Th1 response?
PG  - 50-59
LID - 10.1111/cei.13050 [doi]
AB  - The objective of this study was to evaluate the frequency of CD4(+) T cell 
      subsets in peripheral blood mononuclear cells (PBMC), urine and renal tissue from 
      patients with lupus nephritis (LN). PBMC and urinary cells were collected from 17 
      patients with active LN, 20 disease controls (DC) with primary glomerulonephritis 
      and 10 healthy controls (HC) and were analysed by flow cytometry with markers for 
      T helper type 1 (Th1), Th2, Th17 and regulatory T cells (T(reg) ) cells. T cell 
      subsets were assessed by immunohistochemistry from LN biopsy specimens from 12 LN 
      patients. T cell subtypes in PBMC were re-evaluated at 6 months of therapy. 
      CD4(+) T cells were decreased in PBMC in LN compared with DC and HC (P = 0.0001). 
      No differences were observed in urinary CD4(+) T cell subsets between LN and DC. 
      The frequency of urinary Th17 cells was higher in patients with non-proliferative 
      than in proliferative LN (P = 0.041). CD3(+) and T-box 21 ( Tbet+) cells were 
      found in glomeruli and interstitium of LN patients, while forkhead box protein 3 
      (FoxP3), retinoid-related orphan receptor gamma (ROR-gamma) and GATA binding protein 
      3 (GATA-3) were present only in glomeruli. Th1 cells in PBMC were correlated 
      negatively with urinary Th1 cells (Rho = -0.531; P = 0.028) and with T(bet) in 
      renal interstitium (Rho = -0.782; P = 0.004). At 6 months, LN patients showed an 
      increase in Th17 cells in PBMC. In conclusion, the inverse association between 
      Th1 cells from PBMC and urinary/renal tissue indicate a role for Th1 in LN 
      pathophysiology. Urinary Th17 cells were associated with less severe LN, and Th17 
      increased in PBMC during therapy. Urinary CD4(+) T cells were not different 
      between LN and DC.
CI  - (c) 2017 British Society for Immunology.
FAU - Mesquita, D Jr
AU  - Mesquita D Jr
AD  - Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de Sao 
      Paulo, Sao Paulo-SP, Brazil.
FAU - Kirsztajn, G Mastroianni
AU  - Kirsztajn GM
AD  - Nephrology Division, Escola Paulista de Medicina, Universidade Federal de Sao 
      Paulo, Sao Paulo-SP, Brazil.
FAU - Franco, M F
AU  - Franco MF
AD  - Department of Pathology, Escola Paulista de Medicina, Universidade Federal de Sao 
      Paulo, Sao Paulo-SP, Brazil.
FAU - Reis, L A
AU  - Reis LA
AD  - Nephrology Division, Escola Paulista de Medicina, Universidade Federal de Sao 
      Paulo, Sao Paulo-SP, Brazil.
FAU - Perazzio, S F
AU  - Perazzio SF
AD  - Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de Sao 
      Paulo, Sao Paulo-SP, Brazil.
FAU - Mesquita, F V
AU  - Mesquita FV
AD  - Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de Sao 
      Paulo, Sao Paulo-SP, Brazil.
FAU - Ferreira, V da Silva
AU  - Ferreira VDS
AD  - Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de Sao 
      Paulo, Sao Paulo-SP, Brazil.
FAU - Andrade, L E Coelho
AU  - Andrade LEC
AUID- ORCID: 0000-0001-8742-9931
AD  - Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de Sao 
      Paulo, Sao Paulo-SP, Brazil.
FAU - de Souza, A W Silva
AU  - de Souza AWS
AUID- ORCID: 0000-0001-7681-6215
AD  - Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de Sao 
      Paulo, Sao Paulo-SP, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171010
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Biomarkers)
RN  - 0 (GATA3 Transcription Factor)
SB  - IM
MH  - Adult
MH  - Biomarkers
MH  - Case-Control Studies
MH  - Female
MH  - GATA3 Transcription Factor/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Lupus Nephritis/*immunology/*metabolism/pathology/therapy
MH  - Lymphocyte Count
MH  - Male
MH  - T-Lymphocyte Subsets/immunology/metabolism/pathology
MH  - T-Lymphocytes, Helper-Inducer/*immunology/*metabolism/pathology
MH  - T-Lymphocytes, Regulatory/*immunology/*metabolism/pathology
MH  - Th1 Cells/immunology/metabolism
MH  - Th2 Cells/immunology/metabolism
PMC - PMC5721248
OTO - NOTNLM
OT  - glomerulonephritis
OT  - helper T cells
OT  - lupus nephritis
OT  - regulatory T cells
OT  - systemic lupus erythematosus
EDAT- 2017/09/26 06:00
MHDA- 2017/12/23 06:00
PMCR- 2019/01/01
CRDT- 2017/09/26 06:00
PHST- 2017/09/06 00:00 [accepted]
PHST- 2017/09/26 06:00 [pubmed]
PHST- 2017/12/23 06:00 [medline]
PHST- 2017/09/26 06:00 [entrez]
PHST- 2019/01/01 00:00 [pmc-release]
AID - CEI13050 [pii]
AID - 10.1111/cei.13050 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2018 Jan;191(1):50-59. doi: 10.1111/cei.13050. Epub 2017 Oct 
      10.