PMID- 28945993
OWN - NLM
STAT- MEDLINE
DCOM- 20180502
LR  - 20190122
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 49
DP  - 2017 Nov
TI  - Trans-10,cis-12 conjugated linoleic acid (t10-c12 CLA) treatment and caloric 
      restriction differentially affect adipocyte cell turnover in obese and lean mice.
PG  - 123-132
LID - S0955-2863(17)30046-3 [pii]
LID - 10.1016/j.jnutbio.2017.08.003 [doi]
AB  - Caloric restriction (CR) is one of the most promising strategies for weight loss 
      but is associated with loss of lean mass, whereas compounds such as 
      trans-10,cis-12 conjugated linoleic acid (t10-c12 CLA) have been promoted as 
      antiobesity agents. To compare the mechanisms of weight reduction by CR and 
      t10-c12 CLA, body composition, glucose control, and characteristics of adipose 
      tissue with respect to cell turnover (stem cells and preadipocytes, apoptosis and 
      autophagy) and Tbx-1 localization were examined in obese db/db mice and lean 
      C57BL/6J mice undergoing CR or fed CLA isomers (0.4% w/w c9-t11 or t10-c12) for 4 
      weeks. Our findings show that the t10-c12 CLA reduced whole-body fat mass by 
      decreasing all fat depots (visceral, inguinal, brown/interscapular), while CR 
      lowered both whole-body fat and lean mass in obese mice. t10-c12 CLA elevated 
      blood glucose in both obese and lean mice, while glycemia was not altered by CR. 
      The adipocyte stem cell population remained unchanged; however, t10-c12 CLA 
      reduced and CR elevated the proportion of immature adipocytes in obese mice, 
      suggesting differential effects on adipocyte maturation. t10-c12 CLA reduced 
      apoptosis (activated caspase-3) in both obese and lean mice but did not alter 
      autophagy (LC3II/LC3I). Nuclear Tbx-1, a marker of metabolically active beige 
      adipocytes, was greater in the adipose of t10-c12 CLA-fed animals. Thus, weight 
      loss achieved via t10-c12 CLA primarily involves fat loss and more cells with 
      Tbx-1 localized to the nucleus, while CR operates through a mechanism that 
      reduces both lean and fat mass and blocks adipocyte differentiation.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Yeganeh, Azadeh
AU  - Yeganeh A
AD  - Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, 
      MB, Canada; Canadian Centre for Agri-Food Research in Health and Medicine, St. 
      Boniface Albrechtsen Research Centre, 351 Tache Avenue, Winnipeg, MB, Canada.
FAU - Zahradka, Peter
AU  - Zahradka P
AD  - Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, 
      MB, Canada; Department of Food and Human Nutritional Sciences, University of 
      Manitoba, Winnipeg, MB, Canada; Canadian Centre for Agri-Food Research in Health 
      and Medicine, St. Boniface Albrechtsen Research Centre, 351 Tache Avenue, 
      Winnipeg, MB, Canada.
FAU - Taylor, Carla G
AU  - Taylor CG
AD  - Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, 
      MB, Canada; Department of Food and Human Nutritional Sciences, University of 
      Manitoba, Winnipeg, MB, Canada; Canadian Centre for Agri-Food Research in Health 
      and Medicine, St. Boniface Albrechtsen Research Centre, 351 Tache Avenue, 
      Winnipeg, MB, Canada. Electronic address: ctaylor@sbrc.ca.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170812
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Anti-Obesity Agents)
RN  - 0 (Biomarkers)
RN  - 0 (Linoleic Acids, Conjugated)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (Tbx1 protein, mouse)
RN  - 0 (trans-10,cis-12-conjugated linoleic acid)
SB  - IM
MH  - *Adipogenesis
MH  - Adipose Tissue, Beige/metabolism/pathology
MH  - Adipose Tissue, Brown/metabolism/pathology
MH  - Adipose Tissue, White/metabolism/pathology
MH  - *Adiposity
MH  - Adult Stem Cells/metabolism/pathology
MH  - Animals
MH  - Anti-Obesity Agents/adverse effects/*therapeutic use
MH  - Apoptosis
MH  - Biomarkers/metabolism
MH  - *Caloric Restriction/adverse effects
MH  - *Dietary Supplements/adverse effects
MH  - Linoleic Acids, Conjugated/adverse effects/*therapeutic use
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Obesity/*diet therapy/metabolism/pathology
MH  - Random Allocation
MH  - T-Box Domain Proteins/metabolism
MH  - Weight Loss
OTO - NOTNLM
OT  - Adipocytes
OT  - Caloric restriction
OT  - Cell turnover
OT  - Conjugated linoleic acid
OT  - Weight loss
OT  - db/db mice
EDAT- 2017/09/26 06:00
MHDA- 2018/05/03 06:00
CRDT- 2017/09/26 06:00
PHST- 2017/01/14 00:00 [received]
PHST- 2017/07/14 00:00 [revised]
PHST- 2017/08/07 00:00 [accepted]
PHST- 2017/09/26 06:00 [pubmed]
PHST- 2018/05/03 06:00 [medline]
PHST- 2017/09/26 06:00 [entrez]
AID - S0955-2863(17)30046-3 [pii]
AID - 10.1016/j.jnutbio.2017.08.003 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2017 Nov;49:123-132. doi: 10.1016/j.jnutbio.2017.08.003. Epub 
      2017 Aug 12.