PMID- 28946122 OWN - NLM STAT- MEDLINE DCOM- 20171030 LR - 20171030 IS - 1873-376X (Electronic) IS - 1570-0232 (Linking) VI - 1065-1066 DP - 2017 Oct 15 TI - Quantification of bupivacaine hydrochloride and isoflupredone acetate residues in porcine muscle, beef, milk, egg, shrimp, flatfish, and eel using a simplified extraction method coupled with liquid chromatography-triple quadrupole tandem mass spectrometry. PG - 29-34 LID - S1570-0232(17)31162-5 [pii] LID - 10.1016/j.jchromb.2017.09.026 [doi] AB - In this study, a simple analytical approach has been developed and validated for the determination of bupivacaine hydrochloride and isoflupredone acetate residues in porcine muscle, beef, milk, egg, shrimp, flatfish, and eel using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A 0.1% solution of acetic acid in acetonitrile combined with n-hexane was used for deproteinization and defatting of all tested matrices and the target drugs were well separated on a Waters Xbridge C18 analytical column using a mobile phase consisting of 0.1% acetic acid (A) and 0.1% solution of acetic acid in methanol (B). The linearity estimated from six-point matrix-matched calibrations was good, with coefficients of determination >/=0.9873. The limits of quantification (LOQs) for bupivacaine hydrochloride and isoflupredone acetate were 1 and 2ngg(-1), respectively. Recovery percentages in the ranges of 72.51-112.39% (bupivacaine hydrochloride) and 72.58-114.56% (isoflupredone acetate) were obtained from three different fortification concentrations with relative standard deviations (RSDs) of <15.14%. All samples for the experimental work and method application were collected from the local markets in Seoul, Republic of Korea, and none of them tested positive for the target drugs. In conclusion, a simple method using a 0.1% solution of acetic acid in acetonitrile and n-hexane followed by LC-MS/MS could effectively extract bupivacaine hydrochloride and isoflupredone acetate from porcine muscle, beef, milk, egg, shrimp, flatfish, and eel samples. CI - Copyright (c) 2017 Elsevier B.V. All rights reserved. FAU - Cho, Sang-Hyun AU - Cho SH AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. FAU - Park, Jin-A AU - Park JA AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. FAU - Zheng, Weijia AU - Zheng W AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. FAU - Abd El-Aty, A M AU - Abd El-Aty AM AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea; Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt. Electronic address: abdelaty44@hotmail.com. FAU - Kim, Seong-Kwan AU - Kim SK AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. FAU - Choi, Jeong-Min AU - Choi JM AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. FAU - Yi, Hee AU - Yi H AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. FAU - Cho, Soo-Min AU - Cho SM AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. FAU - Afifi, Nehal A AU - Afifi NA AD - Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt. FAU - Shim, Jae-Han AU - Shim JH AD - Natural Products Chemistry, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Republic of Korea. FAU - Chang, Byung-Joon AU - Chang BJ AD - Department of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. FAU - Kim, Jin-Suk AU - Kim JS AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. FAU - Shin, Ho-Chul AU - Shin HC AD - Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: hshin@konkuk.ac.kr. LA - eng PT - Journal Article DEP - 20170919 PL - Netherlands TA - J Chromatogr B Analyt Technol Biomed Life Sci JT - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JID - 101139554 RN - 55P9TUL75S (isoflupredone acetate) RN - 9H05937G3X (Fluprednisolone) RN - Y8335394RO (Bupivacaine) SB - IM MH - Animals MH - Bupivacaine/*analysis MH - Chromatography, Liquid/methods MH - Drug Residues/*analysis MH - Eels MH - Flatfishes MH - Fluprednisolone/*analogs & derivatives/analysis MH - Linear Models MH - Milk/*chemistry MH - Penaeidae MH - Red Meat/*analysis MH - Reproducibility of Results MH - Seafood/*analysis MH - Sensitivity and Specificity MH - Swine MH - Tandem Mass Spectrometry/methods OTO - NOTNLM OT - Animal-derived food products OT - Bupivacaine OT - Determination OT - Isoflupredone OT - LC-MS/MS OT - Residue EDAT- 2017/09/26 06:00 MHDA- 2017/10/31 06:00 CRDT- 2017/09/26 06:00 PHST- 2017/07/05 00:00 [received] PHST- 2017/09/11 00:00 [revised] PHST- 2017/09/17 00:00 [accepted] PHST- 2017/09/26 06:00 [pubmed] PHST- 2017/10/31 06:00 [medline] PHST- 2017/09/26 06:00 [entrez] AID - S1570-0232(17)31162-5 [pii] AID - 10.1016/j.jchromb.2017.09.026 [doi] PST - ppublish SO - J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Oct 15;1065-1066:29-34. doi: 10.1016/j.jchromb.2017.09.026. Epub 2017 Sep 19.