PMID- 28947251
OWN - NLM
STAT- MEDLINE
DCOM- 20180418
LR  - 20181202
IS  - 1090-2392 (Electronic)
IS  - 0011-2240 (Linking)
VI  - 79
DP  - 2017 Dec
TI  - Supplementation of freezing media with stromal cell-derived factor-1alpha preserves 
      human sperm from cryodamage.
PG  - 37-42
LID - S0011-2240(17)30162-1 [pii]
LID - 10.1016/j.cryobiol.2017.09.004 [doi]
AB  - The destructive effects of sperm cryopreservation result in reduced sperm 
      motility and increased apoptosis. Oocytes, endometrium, and follicular fluid 
      express stromal cell-derived factor-1 alpha (SDF-1alpha) or C-X-C motif chemokine 
      ligand 12 (CXCL12) while its specific receptor chemokine, CXC motif receptor 4 
      (CXCR4) is expressed in the head of sperm. SDF-1alpha can increase sperm motility and 
      preserve normal mitochondrial status. The present study intends to investigate 
      whether the addition of SDF-1alpha to freezing extender can facilitate cryosurvival 
      of spermatozoa and how SDF-1alpha protects spermatozoa against damages during 
      cryopreservation. In this study, we collected 22 semen samples from healthy 
      donors and treated them with different concentrations of SDF-1alpha, followed by 
      cryopreservation for one month. We measured sperm motility by CASA, mitochondrial 
      ROS generation by flow cytometry using the probe MitoSOX Red (MSR) to measure 
      mitochondrial superoxide anion (O2(-)*), DNA fragmentation by flow cytometry 
      according to the TUNEL kit, and expressions of Bcl-2 and Bax by RT-qPCR in 
      freeze-thawed sperm. The results showed that SDF-1alpha attenuated mitochondrial ROS 
      generation at different doses, particularly the 250 ng/ml treated samples which, 
      in turn, reduced the expressions of pro-apoptotic genes such as Bax. Eventually, 
      SDF-1alpha reduced DNA fragmentation and ameliorated sperm motility in the 
      1-100 ng/ml treated samples during cryopreservation. The present study, for the 
      first time, demonstrated that SDF-1alpha dose-dependently moderated oxidative stress 
      injury in human sperm by reduction of mitochondrial ROS generation. It could 
      subsequently cause a decrease in apoptosis during freeze-thawing and protect 
      human spermatozoa from cryodamage.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Hatef, Behnaz
AU  - Hatef B
AD  - Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, 
      Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran. Electronic address: 
      behnaz.hatef@yahoo.com.
FAU - Taromchi, Amirhossein
AU  - Taromchi A
AD  - Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, 
      Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran. Electronic address: 
      Taromchi@yahoo.com.
FAU - Nejatbakhsh, Reza
AU  - Nejatbakhsh R
AD  - Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of 
      Medical Sciences (ZUMS), Zanjan, Iran. Electronic address: Reza_nejat@yahoo.com.
FAU - Farrokhi, Ahmad
AU  - Farrokhi A
AD  - Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of 
      Medical Sciences (ZUMS), Zanjan, Iran.
FAU - Shokri, Saeed
AU  - Shokri S
AD  - Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of 
      Medical Sciences (ZUMS), Zanjan, Iran. Electronic address: saeedshus@zums.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20170922
PL  - Netherlands
TA  - Cryobiology
JT  - Cryobiology
JID - 0006252
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (bcl-2-Associated X Protein)
SB  - IM
MH  - Adult
MH  - Apoptosis/drug effects
MH  - Chemokine CXCL12/*pharmacology
MH  - Cryopreservation/*methods
MH  - Cryoprotective Agents/*pharmacology
MH  - DNA Fragmentation/drug effects
MH  - Freezing
MH  - Humans
MH  - Male
MH  - Mitochondria/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Semen Preservation/*methods
MH  - Sperm Motility/*drug effects
MH  - Spermatozoa/drug effects
MH  - bcl-2-Associated X Protein/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Chemokine CXCL12
OT  - Cryopreservation
OT  - Reactive oxygen species
OT  - Sperm
EDAT- 2017/09/28 06:00
MHDA- 2018/04/19 06:00
CRDT- 2017/09/27 06:00
PHST- 2017/05/06 00:00 [received]
PHST- 2017/09/15 00:00 [revised]
PHST- 2017/09/21 00:00 [accepted]
PHST- 2017/09/28 06:00 [pubmed]
PHST- 2018/04/19 06:00 [medline]
PHST- 2017/09/27 06:00 [entrez]
AID - S0011-2240(17)30162-1 [pii]
AID - 10.1016/j.cryobiol.2017.09.004 [doi]
PST - ppublish
SO  - Cryobiology. 2017 Dec;79:37-42. doi: 10.1016/j.cryobiol.2017.09.004. Epub 2017 
      Sep 22.