PMID- 28947800 OWN - NLM STAT- MEDLINE DCOM- 20180214 LR - 20180214 IS - 2055-0278 (Electronic) IS - 2055-0278 (Linking) VI - 3 IP - 10 DP - 2017 Oct TI - Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis. PG - 814-824 LID - 10.1038/s41477-017-0023-7 [doi] AB - Acetyl-coenzyme A (acetyl-CoA) is a central metabolite and the acetyl source for protein acetylation, particularly histone acetylation that promotes gene expression. However, the effect of acetyl-CoA levels on histone acetylation status in plants remains unknown. Here, we show that malfunctioned cytosolic acetyl-CoA carboxylase1 (ACC1) in Arabidopsis leads to elevated levels of acetyl-CoA and promotes histone hyperacetylation predominantly at lysine 27 of histone H3 (H3K27). The increase of H3K27 acetylation (H3K27ac) is dependent on adenosine triphosphate (ATP)-citrate lyase which cleaves citrate to acetyl-CoA in the cytoplasm, and requires histone acetyltransferase GCN5. A comprehensive analysis of the transcriptome and metabolome in combination with the genome-wide H3K27ac profiles of acc1 mutants demonstrate the dynamic changes in H3K27ac, gene transcripts and metabolites occurring in the cell by the increased levels of acetyl-CoA. This study suggests that H3K27ac is an important link between cytosolic acetyl-CoA level and gene expression in response to the dynamic metabolic environments in plants. FAU - Chen, Chen AU - Chen C AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. AD - Department of Biology, Western University, London, Ontario, N6A 5B7, Canada. FAU - Li, Chenlong AU - Li C AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. AD - State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resource, School of Life Sciences, Sun Yat-sen University, Guangzhou, China. FAU - Wang, Ying AU - Wang Y AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. FAU - Renaud, Justin AU - Renaud J AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. FAU - Tian, Gang AU - Tian G AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. FAU - Kambhampati, Shrikaar AU - Kambhampati S AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. AD - Department of Biology, Western University, London, Ontario, N6A 5B7, Canada. FAU - Saatian, Behnaz AU - Saatian B AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. AD - Department of Biology, Western University, London, Ontario, N6A 5B7, Canada. FAU - Nguyen, Vi AU - Nguyen V AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. FAU - Hannoufa, Abdelali AU - Hannoufa A AUID- ORCID: 0000-0003-0064-620X AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. AD - Department of Biology, Western University, London, Ontario, N6A 5B7, Canada. FAU - Marsolais, Frederic AU - Marsolais F AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. AD - Department of Biology, Western University, London, Ontario, N6A 5B7, Canada. FAU - Yuan, Ze-Chun AU - Yuan ZC AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. FAU - Yu, Kangfu AU - Yu K AD - Agriculture and Agri-Food Canada, Harrow Research and Development Centre, Harrow, Ontario, N0R 1G0, Canada. FAU - Austin, Ryan S AU - Austin RS AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. AD - Department of Biology, Western University, London, Ontario, N6A 5B7, Canada. FAU - Liu, Jun AU - Liu J AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. AD - Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China. FAU - Kohalmi, Susanne E AU - Kohalmi SE AUID- ORCID: 0000-0002-5852-2771 AD - Department of Biology, Western University, London, Ontario, N6A 5B7, Canada. FAU - Wu, Keqiang AU - Wu K AUID- ORCID: 0000-0002-5791-3594 AD - Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei, 106, Taiwan. FAU - Huang, Shangzhi AU - Huang S AD - State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resource, School of Life Sciences, Sun Yat-sen University, Guangzhou, China. FAU - Cui, Yuhai AU - Cui Y AUID- ORCID: 0000-0002-3675-3288 AD - Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada. yuhai.cui@canada.ca. AD - Department of Biology, Western University, London, Ontario, N6A 5B7, Canada. yuhai.cui@canada.ca. LA - eng PT - Journal Article DEP - 20170925 PL - England TA - Nat Plants JT - Nature plants JID - 101651677 RN - 0 (Arabidopsis Proteins) RN - 0 (Histones) RN - 0 (Isoenzymes) RN - 72-89-9 (Acetyl Coenzyme A) RN - EC 6.4.1.2 (Acetyl-CoA Carboxylase) RN - K3Z4F929H6 (Lysine) SB - IM MH - Acetyl Coenzyme A/*metabolism MH - Acetyl-CoA Carboxylase/genetics/metabolism MH - Acetylation MH - Arabidopsis/*metabolism MH - Arabidopsis Proteins/*metabolism MH - Cytosol/metabolism MH - Histones/*metabolism MH - Isoenzymes/genetics/metabolism MH - Lysine/metabolism EDAT- 2017/09/28 06:00 MHDA- 2018/02/15 06:00 CRDT- 2017/09/27 06:00 PHST- 2016/03/04 00:00 [received] PHST- 2017/08/31 00:00 [accepted] PHST- 2017/09/28 06:00 [pubmed] PHST- 2018/02/15 06:00 [medline] PHST- 2017/09/27 06:00 [entrez] AID - 10.1038/s41477-017-0023-7 [pii] AID - 10.1038/s41477-017-0023-7 [doi] PST - ppublish SO - Nat Plants. 2017 Oct;3(10):814-824. doi: 10.1038/s41477-017-0023-7. Epub 2017 Sep 25.