PMID- 28949976
OWN - NLM
STAT- MEDLINE
DCOM- 20171031
LR  - 20220409
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 9
DP  - 2017
TI  - PRCC-TFE3 dual-fusion FISH assay: A new method for identifying PRCC-TFE3 renal 
      cell carcinoma in paraffin-embedded tissue.
PG  - e0185337
LID - 10.1371/journal.pone.0185337 [doi]
LID - e0185337
AB  - PRCC-TFE3 renal cell carcinoma (RCC) is one of the most common types of Xp11.2 
      translocation renal cell carcinoma (tRCC), of which the diagnosis mainly relies 
      on reverse transcription-polymerase chain reaction (RT-PCR) or chromosomal 
      analysis in fresh frozen samples. Herein, we developed a new dual-fusion 
      fluorescence in situ hybridization (FISH) probe to succinctly identify PRCC-TFE3 
      RCC in paraffin-embedded tissue. We immunohistochemically analyzed TFE3 and 
      cathepsin K expression in 23 cases of Xp11.2 tRCC which had been confirmed by 
      break-apart TFE3 FISH probe. Next, the dual-fusion FISH assay was performed on 
      these selected cases. Twenty typical cases of clear renal cell carcinoma and 20 
      cases of papillary renal cell carcinoma were collected as control groups. Seven 
      cases were finally confirmed as PRCC-TFE3 RCC by FISH detection, emerging 
      dual-fusion signals, of which 2 cases were identified as PRCC-TFE3 RCC by RT-PCR 
      previously. All remaining cases were negative for the PRCC-TFE3 rearrangement by 
      FISH. The TFE3 immunohistochemistry was positive in 22/23 cases and the cathepsin 
      K was positive in 16/23 cases. All 7 PRCC-TFE3 RCCs showed positive cathepsin K 
      immunoreactivity. Our results reveal that PRCC-TFE3 dual-fusion FISH probe is an 
      efficient and concise technique for diagnosing PRCC-TFE3 RCC in paraffin-embedded 
      tissue.
FAU - Xiong, Lei
AU  - Xiong L
AD  - Department of Urology, Nanjing Drum Tower Hospital, Medical School of Nanjing 
      University, Institute of Urology, Nanjing University, Nanjing, China.
FAU - Chen, Xiancheng
AU  - Chen X
AD  - Department of Critical Care Medicine, Nanjing Drum Tower Hospital, Medical School 
      of Nanjing University, Nanjing, China.
FAU - Liu, Ning
AU  - Liu N
AD  - Department of Urology, Nanjing Drum Tower Hospital, Medical School of Nanjing 
      University, Institute of Urology, Nanjing University, Nanjing, China.
FAU - Wang, Zhen
AU  - Wang Z
AD  - Department of Urology, Nanjing Drum Tower Hospital, Medical School of Nanjing 
      University, Institute of Urology, Nanjing University, Nanjing, China.
FAU - Miao, Baolei
AU  - Miao B
AD  - Department of Urology, Nanjing Drum Tower Hospital, Medical School of Nanjing 
      University, Institute of Urology, Nanjing University, Nanjing, China.
FAU - Gan, Weidong
AU  - Gan W
AUID- ORCID: 0000-0001-7144-3106
AD  - Department of Urology, Nanjing Drum Tower Hospital, Medical School of Nanjing 
      University, Institute of Urology, Nanjing University, Nanjing, China.
FAU - Li, Dongmei
AU  - Li D
AD  - Immunology and Reproductive Biology Laboratory & State Key Laboratory of 
      Analytical Chemistry for Life Science, Medical School of Nanjing University, 
      Nanjing, China.
AD  - Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, China.
FAU - Guo, Hongqian
AU  - Guo H
AD  - Department of Urology, Nanjing Drum Tower Hospital, Medical School of Nanjing 
      University, Institute of Urology, Nanjing University, Nanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20170926
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (PRCC protein, human)
RN  - 0 (TFE3 protein, human)
SB  - IM
MH  - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/*genetics
MH  - Carcinoma, Renal Cell/*genetics
MH  - Cell Cycle Proteins/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*genetics
MH  - Neoplasm Proteins/*genetics
MH  - Paraffin Embedding
PMC - PMC5614571
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/09/28 06:00
MHDA- 2017/11/01 06:00
PMCR- 2017/09/26
CRDT- 2017/09/27 06:00
PHST- 2017/03/04 00:00 [received]
PHST- 2017/09/11 00:00 [accepted]
PHST- 2017/09/27 06:00 [entrez]
PHST- 2017/09/28 06:00 [pubmed]
PHST- 2017/11/01 06:00 [medline]
PHST- 2017/09/26 00:00 [pmc-release]
AID - PONE-D-17-07703 [pii]
AID - 10.1371/journal.pone.0185337 [doi]
PST - epublish
SO  - PLoS One. 2017 Sep 26;12(9):e0185337. doi: 10.1371/journal.pone.0185337. 
      eCollection 2017.