PMID- 28950692
OWN - NLM
STAT- MEDLINE
DCOM- 20180313
LR  - 20191210
IS  - 0304-4920 (Print)
IS  - 0304-4920 (Linking)
VI  - 60
IP  - 5
DP  - 2017 Oct 31
TI  - Effect of Carvacrol on Ca(2)(+) Movement and Viability in PC3 Human Prostate Cancer 
      Cells.
PG  - 275-283
LID - CJP.2017.BAG506 [pii]
LID - 10.4077/CJP.2017.BAG506 [doi]
AB  - Carvacrol, a monoterpenic phenol compound, has been shown to possess various 
      biological effects in different models. However, the effect of carvacrol on 
      intracellular Ca(2)(+) and its related physiology in human prostate cancer is 
      unknown. This study explored the effect of carvacrol on cytosolic free Ca(2)(+) 
      levels ([Ca(2)(+)]i) and viability in PC3 human prostate cancer cells. Fura-2, a 
      Ca(2)(+)- sensitive fluorescent dye, was used to assess [Ca(2)(+)]i. Cell viability was 
      measured by the detecting reagent WST-1. Carvacrol at concentrations of 200-800 
      muM caused [Ca(2)(+)]i rises in a concentration-dependent manner. Removal of 
      extracellular Ca(2)(+) reduced carvacrol's effect by approximately 60%. 
      Carvacrol-induced Ca(2)(+) entry was confirmed by Mn(2)(+) entry-induced quench of fura-2 
      fluorescence, and was inhibited by approximately 30% by nifedipine, econazole, 
      SKF96365, and the protein kinase C (PKC) inhibitor GF109203X. In Ca(2)(+)-free 
      medium, treatment with the endoplasmic reticulum Ca(2)(+) pump inhibitor thapsigargin 
      (TG) abolished carvacrol-induced [Ca(2)(+)]i rises. Treatment with carvacrol also 
      abolished TG-induced [Ca(2)(+)]i rises. Carvacrol-induced Ca(2)(+) release from the 
      endoplasmic reticulum was abolished by inhibition of phospholipase C (PLC). 
      Carvacrol killed cells at concentrations of 200-600 muM in a 
      concentration-dependent fashion. Chelating cytosolic Ca(2)(+) with BAPTA/AM did not 
      prevent carvacrol's cytotoxicity. Together, in PC3 cells, carvacrol induced 
      [Ca(2)(+)]i rises by inducing PLC-dependent Ca(2)(+) release from the endoplasmic 
      reticulum and Ca(2)(+) entry via PKC-sensitive store-operated Ca(2)(+) channels and other 
      unknown channels. Carvacrol also induced Ca(2)(+)-dissociated cell death.
FAU - Horng, Chi-Ting
AU  - Horng CT
AD  - Department of Ophthalmology, Kaohsiung Armed Force General Hospital, Kaohsiung 
      80284, Taiwan, Republic of China.
FAU - Chou, Chiang-Ting
AU  - Chou CT
AD  - Department of Nursing, Division of Basic Medical Sciences, Chang Gung University 
      of Science and Technology, Chia-Yi 61363, Taiwan, Republic of China.
AD  - Chronic Diseases and Health Promotion Research Center, Chang Gung University of 
      Science and Technology, Chia-Yi 61363, Taiwan, Republic of China.
FAU - Sun, Te-Kung
AU  - Sun TK
AD  - Department of Pediatrics, St. Joseph Hospital, Kaohsiung 80288, Taiwan, Republic 
      of China.
FAU - Liang, Wei-Zhe
AU  - Liang WZ
AD  - Department of Medical Education and Research, Kaohsiung Veterans General 
      Hospital, Kaohsiung 81362, Taiwan, Republic of China.
FAU - Kuo, Chun-Chi
AU  - Kuo CC
AD  - Department of Nursing, Tzu Hui Institute of Technology, Pingtung 92641, Taiwan, 
      Republic of China.
FAU - Wang, Jue-Long
AU  - Wang JL
AD  - Department of Rehabilitation, Kaohsiung Veterans General Hospital Tainan Branch, 
      Tainan 71051, Taiwan, Republic of China.
FAU - Shieh, Pochuen
AU  - Shieh P
AD  - Department of Pharmacy, Tajen University, Pingtung 90741, Taiwan, Republic of 
      China.
FAU - Jan, Chung-Ren
AU  - Jan CR
AD  - Department of Medical Education and Research, Kaohsiung Veterans General 
      Hospital, Kaohsiung 81362, Taiwan, Republic of China.
LA  - eng
GR  - VGHKS105-G01-1, VGHKS105-132/Kaohsiung Veterans General Hospital/International
PT  - Journal Article
PL  - India
TA  - Chin J Physiol
JT  - The Chinese journal of physiology
JID - 7804502
RN  - 0 (Cymenes)
RN  - 0 (Monoterpenes)
RN  - 9B1J4V995Q (carvacrol)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Cymenes
MH  - Humans
MH  - Male
MH  - Monoterpenes/*pharmacology
MH  - Prostatic Neoplasms/*drug therapy/metabolism/pathology
MH  - Type C Phospholipases/physiology
OTO - NOTNLM
OT  - Ca(2)(+)
OT  - carvacrol
OT  - endoplasmic reticulum
OT  - human prostate cancer cells
OT  - store-operated Ca(2)(+) channels
COIS- The authors declare that there are no conflicts of interests.
EDAT- 2017/09/28 06:00
MHDA- 2018/03/14 06:00
CRDT- 2017/09/28 06:00
PHST- 2017/09/28 06:00 [pubmed]
PHST- 2018/03/14 06:00 [medline]
PHST- 2017/09/28 06:00 [entrez]
AID - CJP.2017.BAG506 [pii]
AID - 10.4077/CJP.2017.BAG506 [doi]
PST - ppublish
SO  - Chin J Physiol. 2017 Oct 31;60(5):275-283. doi: 10.4077/CJP.2017.BAG506.