PMID- 28952006
OWN - NLM
STAT- MEDLINE
DCOM- 20180802
LR  - 20181202
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 42
IP  - 12
DP  - 2017 Dec
TI  - Human Umbilical Cord Mesenchymal Stem Cells Overexpressing Nerve Growth Factor 
      Ameliorate Diabetic Cystopathy in Rats.
PG  - 3537-3547
LID - 10.1007/s11064-017-2401-y [doi]
AB  - Diabetic cystopathy is a common complication of voiding disorders in diabetes 
      mellitus. Neuropathy and bladder remodeling underlie the lack of efficacy of 
      pharmacological and surgical treatments. Previous studies have shown that 
      decreased levels of nerve growth factor (NGF) are closely associated with disease 
      progression. Besides, application of human umbilical cord mesenchymal stem cells 
      (hUC-MSCs) is also considered a promising therapeutic strategy for treatment of 
      diabetic neuropathy. In our study, we determine the therapeutic efficacy and 
      mechanisms of hUC-MSCs which transfected with NGF geen in ameliorating diabetic 
      cystopathy for the first time. We transducted hUC-MSCs with NGF-expressing 
      lentivirus so that the hUC-MSCs can express NGF efficiently, then the 
      NGF-expressing hUC-MSCs were intrathecally administrated in L6-S1 spinal cord of 
      diabetic rats 3 days after induced by streptozotocin. Nine weeks later, the level 
      of neurotrophins and voiding function of bladder were detected. Results show that 
      improvements in voiding function were related to the neurotrophins and cytokines 
      released by the intrathecally transplanted hUC-MSCs. In addition, the hUC-MSCs 
      also differentiated into neurons and astrocytes within the spinal cord in rats. 
      These two mechanisms play a combined role in neural regeneration and the 
      amelioration of the symptoms of diabetic cystopathy.
FAU - WenBo, Wu
AU  - WenBo W
AD  - Department of Urology, Shanghai General Hospital (Shanghai Peoples Hosp 1), 
      Shanghai JiaoTong University School of Medicine, 100 Haining Rd, Shanghai, 
      200080, China.
FAU - Fei, Zhang
AU  - Fei Z
AD  - Department of Urology, The Affiliated Hospital of School of Medicine of NingBo 
      University, Ningbo, China.
FAU - YiHeng, Du
AU  - YiHeng D
AD  - Department of Urology, Shanghai General Hospital (Shanghai Peoples Hosp 1), 
      Shanghai JiaoTong University School of Medicine, 100 Haining Rd, Shanghai, 
      200080, China.
FAU - Wei, Wang
AU  - Wei W
AD  - Department of Urology, Shanghai General Hospital (Shanghai Peoples Hosp 1), 
      Shanghai JiaoTong University School of Medicine, 100 Haining Rd, Shanghai, 
      200080, China.
FAU - TingMang, Yan
AU  - TingMang Y
AD  - Department of Urology, Shanghai General Hospital (Shanghai Peoples Hosp 1), 
      Shanghai JiaoTong University School of Medicine, 100 Haining Rd, Shanghai, 
      200080, China.
FAU - WenHao, Zhou
AU  - WenHao Z
AD  - Department of Urology, Shanghai General Hospital (Shanghai Peoples Hosp 1), 
      Shanghai JiaoTong University School of Medicine, 100 Haining Rd, Shanghai, 
      200080, China.
FAU - QianRu, Liu
AU  - QianRu L
AD  - QUFU Normal University, Jining, Shandong, China.
FAU - HaiTao, Liu
AU  - HaiTao L
AD  - Department of Urology, Shanghai General Hospital (Shanghai Peoples Hosp 1), 
      Shanghai JiaoTong University School of Medicine, 100 Haining Rd, Shanghai, 
      200080, China. doctorlht69@163.com.
LA  - eng
GR  - 81170701/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20170926
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (NGF protein, human)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Astrocytes/metabolism
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Diabetes Mellitus, Experimental/*metabolism
MH  - Diabetic Neuropathies/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cell Transplantation/methods
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Nerve Growth Factor/metabolism
MH  - Neurons/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - Diabetic cystopathy
OT  - Functional study
OT  - Human umbilical cord mesenchymal stem cells (hUC-MSCs)
OT  - Intrathecal administration
OT  - Nerve growth factor (NGF)
EDAT- 2017/09/28 06:00
MHDA- 2018/08/03 06:00
CRDT- 2017/09/28 06:00
PHST- 2017/02/07 00:00 [received]
PHST- 2017/09/11 00:00 [accepted]
PHST- 2017/08/10 00:00 [revised]
PHST- 2017/09/28 06:00 [pubmed]
PHST- 2018/08/03 06:00 [medline]
PHST- 2017/09/28 06:00 [entrez]
AID - 10.1007/s11064-017-2401-y [pii]
AID - 10.1007/s11064-017-2401-y [doi]
PST - ppublish
SO  - Neurochem Res. 2017 Dec;42(12):3537-3547. doi: 10.1007/s11064-017-2401-y. Epub 
      2017 Sep 26.