PMID- 28952227
OWN - NLM
STAT- MEDLINE
DCOM- 20190705
LR  - 20190705
IS  - 1107-0625 (Print)
IS  - 1107-0625 (Linking)
VI  - 22
IP  - 4
DP  - 2017 Jul-Aug
TI  - Analysis of the status of EGFR, ROS1 and MET genes in non-small cell lung 
      adenocarcinoma.
PG  - 1053-1060
AB  - PURPOSE: To investigate the status and distribution of epidermal growth factor 
      receptor (EGFR), hepatocyte growth factor receptor (MET), and receptor tyrosine 
      kinase (ROS1) genes in patients with non-small cell lung (NSCL) adenocarcinoma. 
      METHODS: The copy number of the MET gene was detected using fluorescence in situ 
      hybridization (FISH). The splice mutation in exon 14 gene was detected by Sanger 
      sequencing. The mutations in EGFR and the fusion of the ROS1 gene were detected 
      using the fluorescence real-time quantitative PCR method (RT-qPCR). RESULTS: The 
      gene mutation frequency of EGFR was 46.51%. There were 7 types of mutations; exon 
      19 deletions and exon 21 L858R mutations were most frequent. There were 3 cases 
      of double mutations. The MET gene had increased copy numbers in 9.88% of the NSCL 
      adenocarcinoma patients; 3.49% of MET mutations in NSCL adenocarcinoma included 3 
      intron mutations. The ROS1 gene fusion frequency was 1.74%. CONCLUSION: The NSCL 
      adenocarcinoma patients who were females, did not have a smoking history, and had 
      high grade of differentiation, had higher EGFR mutation rates. Although the MET 
      gene amplification and ROS1 gene fusion in NSCL adenocarcinoma were 
      low-probability events, detection of the gene status of EGFR, ROS1, and MET will 
      facilitate screening more NSCL adenocarcinoma patients who might benefit from 
      targeted therapy.
FAU - Wang, Qiong
AU  - Wang Q
AD  - Department of Pathology, General Hospital of PLA, Beijing 100853, China.
FAU - Lv, Yali
AU  - Lv Y
FAU - Zhong, Mei
AU  - Zhong M
FAU - Zhu, Fengwei
AU  - Zhu F
FAU - Wei, Lixin
AU  - Wei L
FAU - Shi, Huaiyin
AU  - Shi H
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.1 (MET protein, human)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (ROS1 protein, human)
SB  - IM
MH  - Adenocarcinoma of Lung/*genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Non-Small-Cell Lung/*genetics
MH  - ErbB Receptors/genetics
MH  - Female
MH  - Gene Amplification/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Lung Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - Mutation/genetics
MH  - Protein-Tyrosine Kinases/*genetics
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proto-Oncogene Proteins c-met/*genetics
EDAT- 2017/09/28 06:00
MHDA- 2019/07/06 06:00
CRDT- 2017/09/28 06:00
PHST- 2017/09/28 06:00 [entrez]
PHST- 2017/09/28 06:00 [pubmed]
PHST- 2019/07/06 06:00 [medline]
PST - ppublish
SO  - J BUON. 2017 Jul-Aug;22(4):1053-1060.