PMID- 28952718
OWN - NLM
STAT- MEDLINE
DCOM- 20190305
LR  - 20190305
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 89
IP  - 21
DP  - 2017 Nov 7
TI  - Quantitative Detection of Trace Level Cloxacillin in Food Samples Using Magnetic 
      Molecularly Imprinted Polymer Extraction and Surface-Enhanced Raman Spectroscopy 
      Nanopillars.
PG  - 11484-11490
LID - 10.1021/acs.analchem.7b02725 [doi]
AB  - There is an increasing demand for rapid, sensitive, and low cost analytical 
      methods to routinely screen antibiotic residues in food products. Conventional 
      detection of antibiotics involves sample preparation by liquid-liquid or 
      solid-phase extraction, followed by analysis using liquid chromatography-mass 
      spectrometry (LC-MS), capillary electrophoresis (CE), or gas chromatography (GC). 
      The process is labor-intensive, time-consuming, and expensive. In this study, we 
      developed a new analytical method that combines magnetic molecularly imprinted 
      polymer (MMIP)-based sample preparation with surface-enhanced Raman spectroscopy 
      (SERS)-based detection for quantitative analysis of cloxacillin in pig serum. 
      MMIP microspheres were synthesized using a core-shell technique. The large 
      loading capacity and high selectivity of the MMIP microspheres enabled efficient 
      extraction of cloxacillin, while the magnetically susceptible characteristics 
      greatly simplified sample handling procedures. Low cost and robust SERS 
      substrates consisting of vertical gold capped silicon nanopillars were fabricated 
      and employed for the detection of cloxacillin. Quantitative SERS was achieved by 
      normalizing signal intensities using an internal standard. By coherently 
      combining MMIP extraction and silicon nanopillar-based SERS biosensor, good 
      sensitivity toward cloxacillin was achieved. The detection limit was 7.8 pmol. 
      Cloxacillin recoveries from spiked pig plasma samples were found to be more than 
      80%.
FAU - Ashley, Jon
AU  - Ashley J
AD  - DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of 
      Denmark , Orsteds Plads, DK-2800, Kongens Lyngby, Denmark.
FAU - Wu, Kaiyu
AU  - Wu K
AUID- ORCID: 0000-0002-5666-8419
AD  - DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of 
      Denmark , Orsteds Plads, DK-2800, Kongens Lyngby, Denmark.
AD  - DNRF and Villum Fonden Center for Intelligent Drug Delivery and Sensing Using 
      Microcontainers and Nanomechanics, IDUN , Orsteds Plads, DK-2800, Kongens Lyngby, 
      Denmark.
FAU - Hansen, Mikkel Fougt
AU  - Hansen MF
AD  - DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of 
      Denmark , Orsteds Plads, DK-2800, Kongens Lyngby, Denmark.
FAU - Schmidt, Michael Stenbaek
AU  - Schmidt MS
AD  - DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of 
      Denmark , Orsteds Plads, DK-2800, Kongens Lyngby, Denmark.
AD  - DNRF and Villum Fonden Center for Intelligent Drug Delivery and Sensing Using 
      Microcontainers and Nanomechanics, IDUN , Orsteds Plads, DK-2800, Kongens Lyngby, 
      Denmark.
FAU - Boisen, Anja
AU  - Boisen A
AD  - DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of 
      Denmark , Orsteds Plads, DK-2800, Kongens Lyngby, Denmark.
AD  - DNRF and Villum Fonden Center for Intelligent Drug Delivery and Sensing Using 
      Microcontainers and Nanomechanics, IDUN , Orsteds Plads, DK-2800, Kongens Lyngby, 
      Denmark.
FAU - Sun, Yi
AU  - Sun Y
AUID- ORCID: 0000-0002-0210-4730
AD  - DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of 
      Denmark , Orsteds Plads, DK-2800, Kongens Lyngby, Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171010
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Polymers)
RN  - O6X5QGC2VB (Cloxacillin)
SB  - IM
MH  - Animals
MH  - Cloxacillin/*analysis/*isolation & purification
MH  - Costs and Cost Analysis
MH  - Drug Residues/analysis
MH  - Food Analysis/economics/*methods
MH  - Food Contamination/analysis
MH  - *Limit of Detection
MH  - Magnets/*chemistry
MH  - Microspheres
MH  - Molecular Imprinting
MH  - Polymers/*chemical synthesis/chemistry
MH  - *Spectrum Analysis, Raman
MH  - Swine
MH  - Time Factors
EDAT- 2017/09/28 06:00
MHDA- 2019/03/06 06:00
CRDT- 2017/09/28 06:00
PHST- 2017/09/28 06:00 [pubmed]
PHST- 2019/03/06 06:00 [medline]
PHST- 2017/09/28 06:00 [entrez]
AID - 10.1021/acs.analchem.7b02725 [doi]
PST - ppublish
SO  - Anal Chem. 2017 Nov 7;89(21):11484-11490. doi: 10.1021/acs.analchem.7b02725. Epub 
      2017 Oct 10.