PMID- 28953957
OWN - NLM
STAT- MEDLINE
DCOM- 20171031
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 9
DP  - 2017
TI  - IgE reactivity to alpha-Gal in relation to Lyme borreliosis.
PG  - e0185723
LID - 10.1371/journal.pone.0185723 [doi]
LID - e0185723
AB  - BACKGROUND: An association between tick bites, the development of immunoglobulin 
      E (IgE) antibodies to galactose-alpha-1, 3-galactose (alpha-Gal) and red meat allergy has 
      recently been reported. Here we wanted to elucidate the relation between tick 
      exposure, IgE antibodies to alpha-Gal and Lyme borreliosis (LB). METHODS: In the 
      highly LB endemic area of Kalmar County, Sweden, serum samples and health 
      inquiries from 518 blood donors were included. All sera were investigated for 
      multiple IgG anti-Borrelia antibodies using a multiplex assay (recomBead, 
      Mikrogen). In addition, three serially collected sera over a six month period 
      from 148 patients with clinically defined erythema migrans (EM) were included. 
      IgE antibodies against alpha-Gal were determined using ImmunoCAP (Thermo Fisher 
      Scientific). RESULTS: In blood donors reporting previous LB (n = 124) IgE to 
      alpha-Gal was found in 16%, while in donors denying previous LB but with multiple 
      anti-Borrelia antibodies (n = 94; interpreted as asymptomatic LB) 10% were IgE 
      alpha-Gal-positive. Finally, in donors without Borrelia antibodies denying previous 
      LB (n = 300) 14% showed IgE to alpha-Gal. No significant difference in proportions 
      among the groups were found. In EM patients, IgE to alpha-Gal was found in 32/148 
      (22%) at diagnosis, 31/148 (21%) after two-three months and 23/148 (16%) after 
      six months. A significant reduction of proportion and level of IgE to alpha-Gal was 
      found between the second and third sample (p<0.01). A positive IgE anti alpha-Gal was 
      more common among men compared with women both in blood donors and in EM patients 
      (p</=0.01). CONCLUSIONS: IgE to alpha-Gal reactivity was common in a tick endemic area 
      but showed no significant relation to previous LB. IgE anti-alpha-Gal reactivity in 
      EM patients peaked within three months of diagnosis of EM, after which it waned 
      indicating that recent tick exposure is of importance in alpha-Gal sensitization. 
      Furthermore, IgE anti alpha-Gal was more common in men compared with women.
FAU - Tjernberg, Ivar
AU  - Tjernberg I
AUID- ORCID: 0000-0001-8657-2496
AD  - Department of Clinical Chemistry and Transfusion Medicine, Kalmar County 
      Hospital, Kalmar, Sweden.
AD  - Department of Clinical and Experimental Medicine, Linkoping University, 
      Linkoping, Sweden.
FAU - Hamsten, Carl
AU  - Hamsten C
AD  - Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet 
      and University Hospital, Stockholm, Sweden.
FAU - Apostolovic, Danijela
AU  - Apostolovic D
AD  - Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet 
      and University Hospital, Stockholm, Sweden.
FAU - van Hage, Marianne
AU  - van Hage M
AD  - Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet 
      and University Hospital, Stockholm, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20170927
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Disaccharides)
RN  - 13168-24-6 (galactosyl-(1-3)galactose)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Blood Donors
MH  - Disaccharides/*immunology
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Lyme Disease/*immunology
PMC - PMC5617217
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/09/28 06:00
MHDA- 2017/11/01 06:00
PMCR- 2017/09/27
CRDT- 2017/09/28 06:00
PHST- 2017/06/26 00:00 [received]
PHST- 2017/09/18 00:00 [accepted]
PHST- 2017/09/28 06:00 [entrez]
PHST- 2017/09/28 06:00 [pubmed]
PHST- 2017/11/01 06:00 [medline]
PHST- 2017/09/27 00:00 [pmc-release]
AID - PONE-D-17-24137 [pii]
AID - 10.1371/journal.pone.0185723 [doi]
PST - epublish
SO  - PLoS One. 2017 Sep 27;12(9):e0185723. doi: 10.1371/journal.pone.0185723. 
      eCollection 2017.