PMID- 28954411 OWN - NLM STAT- MEDLINE DCOM- 20180604 LR - 20181113 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 9 IP - 10 DP - 2017 Sep 26 TI - Beneficial Effects of Small Molecule Oligopeptides Isolated from Panax ginseng Meyer on Pancreatic Beta-Cell Dysfunction and Death in Diabetic Rats. LID - 10.3390/nu9101061 [doi] LID - 1061 AB - To determine whether treatment with ginseng oligopeptides (GOPs) could modulate hyperglycemia related to type 2 diabetes mellitus (T2DM) in rats induced by high-fat diet and low doses of alloxan, type 2 diabetes was induced in male Sprague-Dawley (SD) rats by injecting them once with 105 mg/kg alloxan and feeding them high-carbohydrate/high-fat diet with or without GOP administration (0.125, 0.5, and 2.0 g/kg Body Weight) for 7, 24, and 52 weeks. Oral glucose test tolerance (OGTT), plasma glucose, serum insulin, level of antioxidant, and beta cell function were measured. Morphological observation and immunohistochemistry study of insulin of islets was performed by light microscopy. The insulin level and the expression of NF-kappaB and Bcl-2 family in pancreatic islets were also detected by Western blot analysis. In addition, survival time and survival rate were observed. After the treatment, the abnormal OGTT were partially reversed by GOPs treatment in diabetic rats. The efficacy of GOPs was manifested in the amelioration of pancreatic damage, as determined by microscopy analysis. Moreover, GOPs treatment increased the normal insulin content and decreased the expression of the NF-kappaB-signaling pathway. Compared with those in the control model, the survival time and rate were significantly longer. It is suggested that GOPs exhibit auxiliary therapeutic potential for diabetes. FAU - Xu, Meihong AU - Xu M AD - Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China. xumeihong@bjmu.edu.cn. AD - Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing 100191, China. xumeihong@bjmu.edu.cn. FAU - Sun, Bin AU - Sun B AD - Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China. berseraphim@bjmu.edu.cn. AD - Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing 100191, China. berseraphim@bjmu.edu.cn. FAU - Li, Di AU - Li D AD - Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China. lidiyy@163.com. AD - Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing 100191, China. lidiyy@163.com. FAU - Mao, Ruixue AU - Mao R AD - Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China. rx334@163.com. AD - Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing 100191, China. rx334@163.com. FAU - Li, Hui AU - Li H AD - Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China. huixin6101@163.com. AD - Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing 100191, China. huixin6101@163.com. FAU - Li, Yong AU - Li Y AD - Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China. liyongbmu@163.com. AD - Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing 100191, China. liyongbmu@163.com. FAU - Wang, Junbo AU - Wang J AD - Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China. bmuwjbxy@bjmu.edu.cn. AD - Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing 100191, China. bmuwjbxy@bjmu.edu.cn. LA - eng PT - Journal Article DEP - 20170926 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Antioxidants) RN - 0 (Biomarkers) RN - 0 (Blood Glucose) RN - 0 (Dietary Carbohydrates) RN - 0 (Hypoglycemic Agents) RN - 0 (Insulin) RN - 0 (NF-kappa B) RN - 0 (Oligopeptides) RN - 0 (Plant Extracts) RN - 0 (Proto-Oncogene Proteins c-bcl-2) RN - 6SW5YHA5NG (Alloxan) SB - IM MH - Alloxan MH - Animals MH - Antioxidants/metabolism MH - Biomarkers/blood MH - Blood Glucose/*drug effects/metabolism MH - Diabetes Mellitus, Experimental/blood/*drug therapy/pathology MH - Diabetes Mellitus, Type 2/blood/*drug therapy/pathology MH - Diet, High-Fat MH - Dietary Carbohydrates MH - Hypoglycemic Agents/isolation & purification/*pharmacology MH - Insulin/blood MH - Insulin-Secreting Cells/*drug effects/metabolism/pathology MH - Male MH - NF-kappa B/metabolism MH - Oligopeptides/isolation & purification/*pharmacology MH - *Panax/chemistry MH - Phytotherapy MH - Plant Extracts/isolation & purification/*pharmacology MH - Plant Roots MH - Plants, Medicinal MH - Proto-Oncogene Proteins c-bcl-2/metabolism MH - Rats, Sprague-Dawley MH - Time Factors PMC - PMC5691678 OTO - NOTNLM OT - Panax ginseng oligopeptide OT - alloxan OT - high-carbohydrate/high-fat OT - pancreatic beta-cell failure OT - type 2 diabetes mellitus COIS- The authors declare no conflict of interest. EDAT- 2017/09/29 06:00 MHDA- 2018/06/05 06:00 PMCR- 2017/10/01 CRDT- 2017/09/29 06:00 PHST- 2017/05/27 00:00 [received] PHST- 2017/09/21 00:00 [revised] PHST- 2017/09/22 00:00 [accepted] PHST- 2017/09/29 06:00 [entrez] PHST- 2017/09/29 06:00 [pubmed] PHST- 2018/06/05 06:00 [medline] PHST- 2017/10/01 00:00 [pmc-release] AID - nu9101061 [pii] AID - nutrients-09-01061 [pii] AID - 10.3390/nu9101061 [doi] PST - epublish SO - Nutrients. 2017 Sep 26;9(10):1061. doi: 10.3390/nu9101061.