PMID- 28954602
OWN - NLM
STAT- MEDLINE
DCOM- 20181106
LR  - 20181106
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 25
IP  - 7
DP  - 2018 Jul
TI  - Role of Brain-Derived Neurotrophic Factor in Endometriosis Pain.
PG  - 1045-1057
LID - 10.1177/1933719117732161 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is a regulator for the formation and 
      maintenance of chronic pain in various chronic disorders and has been shown to 
      increase in the serum of women with endometriosis. However, BDNF expression in 
      the peritoneal fluid (PF) and ectopic lesions and its role in endometriosis pain 
      remain unclear. Thus, this study aims to determine the BDNF concentrations in 
      serum and PFs and BDNF expression levels in ectopic lesions and endometriotic 
      stromal cells (ESCs) of women with endometriosis (n = 60). The obtained results 
      were then compared with those of women without endometriosis (n = 38). 
      Brain-derived neurotrophic factor concentrations in serum and PF, as well as the 
      BDNF expression levels in ectopic lesions and endometriotic cells, were evaluated 
      through enzyme-linked immunosorbent assay, immunohistochemical staining, 
      quantitative real-time polymerase chain reaction, and Western blot analysis. As a 
      result, BDNF concentrations in serum and PF were significantly higher in women 
      with endometriosis with pain (2284.3 +/- 51.5 pg/mL, n = 23; 58.8 +/- 6.4 pg/mL, n = 
      16) than in women with endometriosis without pain (1999.8 +/- 61.1 pg/mL, n = 37; 
      31.7 +/- 2.9 pg/mL, n = 25; P < .01). Moreover, BDNF messenger RNA (mRNA) 
      expression levels in ectopic lesions (8.97 +/- 1.44, n = 29) were significantly 
      higher than eutopic (0.97 +/- 0.14, n = 16; P < .01) and control endometrium (1.23 
      +/- 0.19, n = 18; P < .01) and were correlated with endometriosis pain ( P < .05). 
      Furthermore, increased BDNF mRNA and protein expression levels in ESCs induced by 
      estradiol or interleukin 1beta were removed using a phosphorylated 
      extracellular-regulated protein kinase 1/2 inhibitor. These results suggest that 
      BDNF may play an important role in the pathogenesis of endometriosis pain.
FAU - Ding, Shaojie
AU  - Ding S
AD  - 1 Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 
      People's Republic of China.
FAU - Zhu, Tianhong
AU  - Zhu T
AD  - 1 Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 
      People's Republic of China.
FAU - Tian, Yonghong
AU  - Tian Y
AD  - 1 Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 
      People's Republic of China.
FAU - Xu, Ping
AU  - Xu P
AD  - 1 Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 
      People's Republic of China.
FAU - Chen, Zhengyun
AU  - Chen Z
AD  - 1 Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 
      People's Republic of China.
FAU - Huang, Xiufeng
AU  - Huang X
AD  - 1 Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 
      People's Republic of China.
FAU - Zhang, Xinmei
AU  - Zhang X
AD  - 1 Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170927
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Adult
MH  - Ascitic Fluid/metabolism
MH  - Brain-Derived Neurotrophic Factor/blood/*metabolism
MH  - Cells, Cultured
MH  - Endometriosis/complications/*metabolism
MH  - Endometrium/metabolism/pathology
MH  - Female
MH  - Humans
MH  - Infertility, Female/complications/metabolism
MH  - Pain/complications/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Stromal Cells/metabolism/pathology
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - endometriosis
OT  - extracellular-regulated protein kinase
OT  - pain
OT  - tyrosine receptor kinase B
EDAT- 2017/09/29 06:00
MHDA- 2018/11/07 06:00
CRDT- 2017/09/29 06:00
PHST- 2017/09/29 06:00 [pubmed]
PHST- 2018/11/07 06:00 [medline]
PHST- 2017/09/29 06:00 [entrez]
AID - 10.1177/1933719117732161 [doi]
PST - ppublish
SO  - Reprod Sci. 2018 Jul;25(7):1045-1057. doi: 10.1177/1933719117732161. Epub 2017 
      Sep 27.