PMID- 28955336
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 8
DP  - 2017
TI  - Increased Neutralizing Antibody Production and Interferon-gamma Secretion in Response 
      to Porcine Reproductive and Respiratory Syndrome Virus Immunization in 
      Genetically Modified Pigs.
PG  - 1110
LID - 10.3389/fimmu.2017.01110 [doi]
LID - 1110
AB  - T cell-mediated immunity plays a prominent role in combating pathogens infection. 
      Both the engagement of the T cell receptor with the peptide-bound major 
      histocompatibility complex and a costimulatory signal are needed for the complete 
      activation of the T cell. To determine whether host immune responses to 
      vaccination could be improved by enhancing CD28-mediated costimulation and verify 
      whether the boosted immune responses could protect the host against viral 
      challenge, we produced a transgenic pig line expressing an extra copy of the CD28 
      gene controlled by its own promoter at the Rosa26 locus. As expected, in response 
      to porcine reproductive and respiratory syndrome virus (PRRSV) strain 
      vaccination, CD4(+) T cells was remarkably increased in CD28 transgenic pigs and 
      a similar response in CD8(+) T cells was elicited after challenge. Importantly, 
      because of increased T cell frequencies, the virus-neutralizing antibody against 
      JXA-1 (a highly pathogenic Chinese PRRSV strain), as well as interferon-gamma 
      secretion, were enhanced in transgenic pigs. These findings in our translational 
      study provide a novel concept for farm animal breeding in disease resistance, in 
      which we may use the transgenic technology to force overexpression of confirmed 
      immunity-promoting molecules like CD28 and produce an animal with enhanced immune 
      responses to vaccination and broad-spectrum resistance to infectious diseases.
FAU - Huang, Guangping
AU  - Huang G
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
AD  - College of Veterinary Medicine, China Agricultural University, Beijing, China.
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing, 
      China.
FAU - Liu, Xianyong
AU  - Liu X
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
AD  - College of Veterinary Medicine, China Agricultural University, Beijing, China.
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing, 
      China.
FAU - Tang, Xiaoli
AU  - Tang X
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
AD  - College of Veterinary Medicine, China Agricultural University, Beijing, China.
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing, 
      China.
FAU - Du, Li
AU  - Du L
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
AD  - Department of Microbiology and Immunology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
FAU - Feng, Wenhai
AU  - Feng W
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
AD  - Department of Microbiology and Immunology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
FAU - Hu, Xiaoxiang
AU  - Hu X
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
FAU - Zhu, Liangquan
AU  - Zhu L
AD  - China Institute of Veterinary Drug Control, Beijing, China.
FAU - Li, Qiuyan
AU  - Li Q
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
FAU - Suo, Xun
AU  - Suo X
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
AD  - College of Veterinary Medicine, China Agricultural University, Beijing, China.
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20170912
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
PMC - PMC5600919
OTO - NOTNLM
OT  - CD28
OT  - antiviral immune responses
OT  - interferon-gamma
OT  - neutralizing antibody
OT  - transgenic pig
EDAT- 2017/09/29 06:00
MHDA- 2017/09/29 06:01
PMCR- 2017/01/01
CRDT- 2017/09/29 06:00
PHST- 2017/07/12 00:00 [received]
PHST- 2017/08/24 00:00 [accepted]
PHST- 2017/09/29 06:00 [entrez]
PHST- 2017/09/29 06:00 [pubmed]
PHST- 2017/09/29 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2017.01110 [doi]
PST - epublish
SO  - Front Immunol. 2017 Sep 12;8:1110. doi: 10.3389/fimmu.2017.01110. eCollection 
      2017.