PMID- 28955629
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240327
IS  - 2190-572X (Print)
IS  - 2190-5738 (Electronic)
IS  - 2190-5738 (Linking)
VI  - 7
IP  - 5
DP  - 2017 Oct
TI  - Biodegradation of thermally treated high-density polyethylene (HDPE) by 
      Klebsiella pneumoniae CH001.
PG  - 332
LID - 10.1007/s13205-017-0959-3 [doi]
LID - 332
AB  - Biodegradation of plastics, which are the potential source of environmental 
      pollution, has received a great deal of attention in the recent years. We aim to 
      screen, identify, and characterize a bacterial strain capable of degrading 
      high-density polyethylene (HDPE). In the present study, we studied HDPE 
      biodegradation using a laboratory isolate, which was identified as Klebsiella 
      pneumoniae CH001 (Accession No MF399051). The HDPE film was characterized by 
      Universal Tensile Machine (UTM), Fourier Transform Infrared Spectroscopy (FTIR), 
      Scanning Electron Microscope (SEM), and Atomic Force Microscope (AFM) before and 
      after microbial incubation. We observed that this strain was capable of adhering 
      strongly on HDPE surface and form a thick biofilm, when incubated in nutrient 
      broth at 30  degrees C on 120 rpm for 60 days. UTM analysis showed a significant decrease 
      in weight (18.4%) and reduction in tensile strength (60%) of HDPE film. 
      Furthermore, SEM analysis showed the cracks on the HDPE surface, whereas AFM 
      results showed an increase in surface roughness after bacterial incubation. 
      Overall, these results indicate that K. pneumoniae CH001 can be used as potential 
      candidate for HDPE degradation in eco-friendly and sustainable manner in the 
      environment.
FAU - Awasthi, Shraddha
AU  - Awasthi S
AUID- ORCID: 0000-0002-8118-1493
AD  - Department of Chemistry, Indian Institute of Technology (BHU), Varanasi, U.P. 
      221005 India. GRID: grid.467228.d
FAU - Srivastava, Pratap
AU  - Srivastava P
AD  - Department of Chemical Engineering and Technology, Indian Institute of Technology 
      (BHU), Varanasi, U.P. 221005 India. GRID: grid.467228.d
FAU - Singh, Pardeep
AU  - Singh P
AD  - Department of Chemistry, Indian Institute of Technology (BHU), Varanasi, U.P. 
      221005 India. GRID: grid.467228.d
FAU - Tiwary, D
AU  - Tiwary D
AD  - Department of Chemistry, Indian Institute of Technology (BHU), Varanasi, U.P. 
      221005 India. GRID: grid.467228.d
FAU - Mishra, Pradeep Kumar
AU  - Mishra PK
AD  - Department of Chemical Engineering and Technology, Indian Institute of Technology 
      (BHU), Varanasi, U.P. 221005 India. GRID: grid.467228.d
LA  - eng
PT  - Journal Article
DEP - 20170919
PL  - Germany
TA  - 3 Biotech
JT  - 3 Biotech
JID - 101565857
PMC - PMC5603451
OTO - NOTNLM
OT  - Biodegradation
OT  - Eco-friendly
OT  - High-density polyethylene
OT  - Klebsiella Pneumoniae
OT  - Plastic
OT  - Sustainable
COIS- CONFLICT OF INTEREST: There are no conflicts of interest between authors. ETHICAL 
      APPROVAL: There is no environment of human cell or animal cell in this work.
EDAT- 2017/09/29 06:00
MHDA- 2017/09/29 06:01
PMCR- 2018/10/01
CRDT- 2017/09/29 06:00
PHST- 2017/05/18 00:00 [received]
PHST- 2017/09/07 00:00 [accepted]
PHST- 2017/09/29 06:00 [entrez]
PHST- 2017/09/29 06:00 [pubmed]
PHST- 2017/09/29 06:01 [medline]
PHST- 2018/10/01 00:00 [pmc-release]
AID - 959 [pii]
AID - 10.1007/s13205-017-0959-3 [doi]
PST - ppublish
SO  - 3 Biotech. 2017 Oct;7(5):332. doi: 10.1007/s13205-017-0959-3. Epub 2017 Sep 19.