PMID- 28955961
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2405-5808 (Print)
IS  - 2405-5808 (Electronic)
IS  - 2405-5808 (Linking)
VI  - 8
DP  - 2016 Dec
TI  - Kynurenine aminotransferase 3/glutamine transaminase L/cysteine conjugate 
      beta-lyase 2 is a major glutamine transaminase in the mouse kidney.
PG  - 234-241
LID - 10.1016/j.bbrep.2016.09.008 [doi]
AB  - BACKGROUND: Kynurenine aminotransferase 3 (KAT3) catalyzes the transamination of 
      Kynurenine to kynurenic acid, and is identical to cysteine conjugate beta-lyase 2 
      (CCBL2) and glutamine transaminase L (GTL). GTL was previously purified from the 
      rat liver and considered as a liver type glutamine transaminase. However, because 
      of the substrate overlap and high sequence similarity of KAT3 and KAT1, it was 
      difficult to assay the specific activity of each KAT and to study the enzyme 
      localization in animals. METHODS: KAT3 transcript and protein levels as well as 
      enzyme activity in the liver and kidney were analyzed by regular reverse 
      transcription-polymerase chain reaction (RT-PCR), real time RT-PCR, biochemical 
      activity assays combined with a specific inhibition assay, and western blotting 
      using a purified and a highly specific antibody, respectively. RESULTS: This 
      study concerns the comparative biochemical characterization and localization of 
      KAT 3 in the mouse. The results showed that KAT3 was present in both liver and 
      kidney of the mouse, but was much more abundant in the kidney than in the liver. 
      The mouse KAT3 is more efficient in transamination of glutamine with 
      indo-3-pyruvate or oxaloacetate as amino group acceptor than the mouse KAT1. 
      CONCLUSIONS: Mouse KAT3 is a major glutamine transaminase in the kidney although 
      it was named a liver type transaminase. GENERAL SIGNIFICANCE: Our data highlights 
      KAT3 as a key enzyme for studying the nephrotoxic mechanism of some xenobiotics 
      and the formation of chemopreventive compounds in the mouse kidney. This suggests 
      tissue localizations of KAT3/GTL/CCBL2 in other animals may be carefully checked.
FAU - Yang, Cihan
AU  - Yang C
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan 
      University, Haikou, Hainan 570228, China.
AD  - Laboratory of Tropical Veterinary Medicine and Vector Biology, College of 
      Agriculture, Hainan University, Haikou, Hainan 570228, China.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan 
      University, Haikou, Hainan 570228, China.
AD  - Laboratory of Tropical Veterinary Medicine and Vector Biology, College of 
      Agriculture, Hainan University, Haikou, Hainan 570228, China.
FAU - Han, Qian
AU  - Han Q
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan 
      University, Haikou, Hainan 570228, China.
AD  - Laboratory of Tropical Veterinary Medicine and Vector Biology, College of 
      Agriculture, Hainan University, Haikou, Hainan 570228, China.
AD  - Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA.
FAU - Liao, Chenghong
AU  - Liao C
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan 
      University, Haikou, Hainan 570228, China.
AD  - Laboratory of Tropical Veterinary Medicine and Vector Biology, College of 
      Agriculture, Hainan University, Haikou, Hainan 570228, China.
FAU - Lan, Jianqiang
AU  - Lan J
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan 
      University, Haikou, Hainan 570228, China.
AD  - Laboratory of Tropical Veterinary Medicine and Vector Biology, College of 
      Agriculture, Hainan University, Haikou, Hainan 570228, China.
FAU - Ding, Haizhen
AU  - Ding H
AD  - Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA.
FAU - Zhou, Hailong
AU  - Zhou H
AD  - Laboratory of Tropical Veterinary Medicine and Vector Biology, College of 
      Agriculture, Hainan University, Haikou, Hainan 570228, China.
FAU - Diao, Xiaoping
AU  - Diao X
AD  - Laboratory of Tropical Veterinary Medicine and Vector Biology, College of 
      Agriculture, Hainan University, Haikou, Hainan 570228, China.
FAU - Li, Jianyong
AU  - Li J
AD  - Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA.
LA  - eng
PT  - Journal Article
DEP - 20160921
PL  - Netherlands
TA  - Biochem Biophys Rep
JT  - Biochemistry and biophysics reports
JID - 101660999
PMC - PMC5613967
OTO - NOTNLM
OT  - Aminotransferase
OT  - CCBL, cysteine conjugate beta-lyase
OT  - Cysteine conjugate beta-lyase 2
OT  - GTK, glutamine transaminase K
OT  - GTL, glutamine transaminase L
OT  - Glutamine transaminase
OT  - KAT, kynurenine aminotransferase
OT  - KYNA, kynurenic acid
OT  - Kynurenic acid
OT  - Kynurenine
OT  - Kynurenine aminotransferase
OT  - PLP, pyridoxal-5'-phosphate.
EDAT- 2017/09/29 06:00
MHDA- 2017/09/29 06:01
PMCR- 2016/09/21
CRDT- 2017/09/29 06:00
PHST- 2016/08/22 00:00 [received]
PHST- 2016/09/19 00:00 [revised]
PHST- 2016/09/19 00:00 [accepted]
PHST- 2017/09/29 06:00 [entrez]
PHST- 2017/09/29 06:00 [pubmed]
PHST- 2017/09/29 06:01 [medline]
PHST- 2016/09/21 00:00 [pmc-release]
AID - S2405-5808(16)30189-3 [pii]
AID - 10.1016/j.bbrep.2016.09.008 [doi]
PST - epublish
SO  - Biochem Biophys Rep. 2016 Sep 21;8:234-241. doi: 10.1016/j.bbrep.2016.09.008. 
      eCollection 2016 Dec.