PMID- 28956034
OWN - NLM
STAT- MEDLINE
DCOM- 20180606
LR  - 20180606
IS  - 1742-2051 (Electronic)
IS  - 1742-2051 (Linking)
VI  - 13
IP  - 11
DP  - 2017 Oct 24
TI  - Metabolomics reveal mitochondrial and fatty acid metabolism disorders that 
      contribute to the development of DKD in T2DM patients.
PG  - 2392-2400
LID - 10.1039/c7mb00167c [doi]
AB  - Diabetic kidney disease (DKD) is the leading cause of ESRD; however, early 
      intervention can greatly prevent the progression of DKD; thus, sensitive 
      biomarkers for DKD are still required. This study was aimed at the identification 
      of potential biomarkers and revelation of underlying pathways in DKD patients by 
      non-targeted metabolomics. Gas chromatography-mass spectrometry was used to 
      analyze urine obtained from the control and type 2 diabetes mellitus (T2DM) and 
      DKD patients, and the renal histological changes in DKD patients were assessed. 
      The DKD group showed increased level of uric acid, 1,5-anhydroglucitol, hippuric 
      acid, stearic acid, and palmitic acid and reduced level of uracil, glycine, 
      aconitic acid, isocitric acid, 4-hydroxybutyrate, 2-deoxyerythritol, and glycolic 
      acid as compared to the control and T2DM groups. Further analysis indicated that 
      many of the changed metabolites were involved in mitochondrial and fatty acid 
      (FA) metabolism, and combined mitochondrial and FA metabolites showed better 
      diagnosis values for DKD. Histological results confirmed that renal expression of 
      key proteins was reduced in DKD patients with respect to mitochondrial biogenesis 
      (PGC-1alpha, p-AMPK) and FA oxidation (PPAR-alpha, CPT-1) as compared to that in the 
      control and T2DM groups. This study highlighted that both mitochondrial and FA 
      metabolism were disturbed in DKD, and thus, they could serve as combined 
      biomarkers for the prediction of DKD.
FAU - Li, Ling
AU  - Li L
AD  - Kidney Research Institute, Division of Nephrology, West China Hospital of Sichuan 
      University, Chengdu, China. fupinghx@163.com.
FAU - Wang, Chengshi
AU  - Wang C
FAU - Yang, Hongliu
AU  - Yang H
FAU - Liu, Shuyun
AU  - Liu S
FAU - Lu, Yanrong
AU  - Lu Y
FAU - Fu, Ping
AU  - Fu P
FAU - Liu, Jingping
AU  - Liu J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Mol Biosyst
JT  - Molecular bioSystems
JID - 101251620
RN  - 0 (Biomarkers)
RN  - 0 (Fatty Acids)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers
MH  - Diabetes Mellitus, Type 2/*etiology/metabolism/pathology
MH  - Diabetic Nephropathies/*etiology/metabolism/pathology
MH  - Fatty Acids/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Metabolic Diseases/*complications/*metabolism
MH  - *Metabolomics/methods
MH  - Middle Aged
MH  - Mitochondrial Diseases/*complications/*metabolism
MH  - ROC Curve
EDAT- 2017/09/29 06:00
MHDA- 2018/06/07 06:00
CRDT- 2017/09/29 06:00
PHST- 2017/09/29 06:00 [pubmed]
PHST- 2018/06/07 06:00 [medline]
PHST- 2017/09/29 06:00 [entrez]
AID - 10.1039/c7mb00167c [doi]
PST - ppublish
SO  - Mol Biosyst. 2017 Oct 24;13(11):2392-2400. doi: 10.1039/c7mb00167c.