PMID- 28957720
OWN - NLM
STAT- MEDLINE
DCOM- 20180703
LR  - 20180703
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 148
DP  - 2018 Jan 30
TI  - Application of gas chromatography-tandem mass spectrometry for the determination 
      of amphetamine-type stimulants in blood and urine.
PG  - 58-64
LID - S0731-7085(17)31816-2 [pii]
LID - 10.1016/j.jpba.2017.09.020 [doi]
AB  - Amphetamine, methamphetamine, phentermine, 3,4-methylenedioxyamphetamine (MDA), 
      3,4-methylenedioxymethamphetamine (MDMA), and 
      3,4-methylenedioxy-N-ethylamphetamine (MDEA) are the most popular 
      amphetamine-type stimulants. The use of these substances is a serious societal 
      problem worldwide. In this study, a method based on gas chromatography-tandem 
      mass spectrometry (GC-MS/MS) with simple and rapid liquid-liquid extraction (LLE) 
      and derivatization was developed and validated for the simultaneous determination 
      of the six aforementioned amphetamine derivatives in blood and urine. The 
      detection of all compounds was based on multiple reaction monitoring (MRM) 
      transitions. The most important advantage of the method is the minimal sample 
      volume (as low as 200muL) required for the extraction procedure. The validation 
      parameters, i.e., the recovery (90.5-104%), inter-day accuracy (94.2-109.1%) and 
      precision (0.5-5.8%), showed the repeatability and sensitivity of the method for 
      both matrices and indicated that the proposed procedure fulfils internationally 
      established acceptance criteria for bioanalytical methods The procedure was 
      successfully applied to the analysis of real blood and urine samples examined in 
      22 forensic toxicological cases. To the best of our knowledge, this is the first 
      work presenting the use of GC-MS/MS for the determination of amphetamine-type 
      stimulants in blood and urine. In view of the low limits of detection 
      (0.09-0.81ng/mL), limits of quantification (0.26-2.4ng/mL), and high selectivity, 
      the procedure can be applied for drug monitoring in both fatal and non-fatal 
      intoxication cases in routine toxicology analysis.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Wozniak, Mateusz Kacper
AU  - Wozniak MK
AD  - Department of Analytical Chemistry, Faculty of Chemistry, Gdansk University of 
      Technology, 11/12 Narutowicza Str., Gdansk 80-233, Poland. Electronic address: 
      womat90@gmail.com.
FAU - Wiergowski, Marek
AU  - Wiergowski M
AD  - Department of Forensic Medicine, Faculty of Medicine, Medical University of 
      Gdansk, 3A Marii Sklodowskiej-Curie Str., Gdansk 80-210, Poland.
FAU - Aszyk, Justyna
AU  - Aszyk J
AD  - Department of Analytical Chemistry, Faculty of Chemistry, Gdansk University of 
      Technology, 11/12 Narutowicza Str., Gdansk 80-233, Poland.
FAU - Kubica, Pawel
AU  - Kubica P
AD  - Department of Analytical Chemistry, Faculty of Chemistry, Gdansk University of 
      Technology, 11/12 Narutowicza Str., Gdansk 80-233, Poland.
FAU - Namiesnik, Jacek
AU  - Namiesnik J
AD  - Department of Analytical Chemistry, Faculty of Chemistry, Gdansk University of 
      Technology, 11/12 Narutowicza Str., Gdansk 80-233, Poland.
FAU - Biziuk, Marek
AU  - Biziuk M
AD  - Department of Analytical Chemistry, Faculty of Chemistry, Gdansk University of 
      Technology, 11/12 Narutowicza Str., Gdansk 80-233, Poland.
LA  - eng
PT  - Journal Article
DEP - 20170918
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Central Nervous System Stimulants)
RN  - CK833KGX7E (Amphetamine)
SB  - IM
MH  - Amphetamine/*blood/*urine
MH  - Central Nervous System Stimulants/*blood/*urine
MH  - Gas Chromatography-Mass Spectrometry/methods
MH  - Humans
MH  - Limit of Detection
MH  - Liquid-Liquid Extraction/methods
MH  - Reproducibility of Results
MH  - Substance Abuse Detection/methods
MH  - Tandem Mass Spectrometry/methods
OTO - NOTNLM
OT  - Amphetamine-type stimulants (ATS)
OT  - Biological specimens
OT  - Drug analysis
OT  - GC-MS/MS
OT  - Toxicological screening
EDAT- 2017/09/29 06:00
MHDA- 2018/07/04 06:00
CRDT- 2017/09/29 06:00
PHST- 2017/07/14 00:00 [received]
PHST- 2017/09/13 00:00 [revised]
PHST- 2017/09/15 00:00 [accepted]
PHST- 2017/09/29 06:00 [pubmed]
PHST- 2018/07/04 06:00 [medline]
PHST- 2017/09/29 06:00 [entrez]
AID - S0731-7085(17)31816-2 [pii]
AID - 10.1016/j.jpba.2017.09.020 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2018 Jan 30;148:58-64. doi: 10.1016/j.jpba.2017.09.020. Epub 
      2017 Sep 18.