PMID- 28958614
OWN - NLM
STAT- MEDLINE
DCOM- 20180730
LR  - 20200106
IS  - 2299-5684 (Electronic)
IS  - 1734-1140 (Linking)
VI  - 69
IP  - 5
DP  - 2017 Oct
TI  - Thiamine and riboflavin inhibit production of cytokines and increase the 
      anti-inflammatory activity of a corticosteroid in a chronic model of inflammation 
      induced by complete Freund's adjuvant.
PG  - 1036-1043
LID - S1734-1140(16)30272-9 [pii]
LID - 10.1016/j.pharep.2017.04.011 [doi]
AB  - BACKGROUND: The effects induced by thiamine and riboflavin, isolated or in 
      association with corticosteroids, in models of chronic inflammation are not 
      known. Thus, we evaluated the effect induced by these B vitamins, isolated or in 
      association with dexamethasone, on the mechanical allodynia, paw edema and 
      cytokine production induced by complete Freund's adjuvant (CFA) in rats. METHODS: 
      Chronic inflammation was induced by two injections of CFA. Nociceptive threshold, 
      paw volume and body temperature were evaluated for 21days. Tumor necrosis 
      factor-alpha (TNF-alpha) and interleukin-6 (IL-6) contents were determined in paw tissue. 
      Riboflavin (125, 250 or 500mg/kg) or thiamine (150, 300 or 600mg/kg) were 
      administered per os (po), twice daily. Dexamethasone (0.5mg/kgday, po) was 
      administered every three days. RESULTS: CFA induced long lasting mechanical 
      allodynia and paw edema. Elevation of body temperature was observed for a short 
      period. Riboflavin reduced neither paw edema nor mechanical allodynia. Thiamine 
      did not change paw edema, but partially inhibited mechanical allodynia. 
      Riboflavin (500mg/kg) and thiamine (600mg/kg) exacerbated the anti-inflammatory 
      activity of dexamethasone. Riboflavin, thiamine and dexamethasone reduced TNF-alpha 
      and IL-6 production. The association of dexamethasone with thiamine induced 
      greater inhibition of IL-6 production when compared with that induced by 
      dexamethasone. CONCLUSIONS: Riboflavin and thiamine exacerbate the 
      anti-inflammatory activity of dexamethasone and reduce production of TNF-alpha and 
      IL-6.
CI  - Copyright (c) 2017 Institute of Pharmacology, Polish Academy of Sciences. Published 
      by Elsevier Urban & Partner Sp. z o.o. All rights reserved.
FAU - Menezes, Raquel R
AU  - Menezes RR
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Godin, Adriana M
AU  - Godin AM
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Rodrigues, Felipe Fernandes
AU  - Rodrigues FF
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Coura, Giovanna M E
AU  - Coura GME
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Melo, Ivo S F
AU  - Melo ISF
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Brito, Ana Mercy S
AU  - Brito AMS
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Bertollo, Caryne M
AU  - Bertollo CM
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Paulino, Tony P
AU  - Paulino TP
AD  - Centro de Formacao Especial em Saude, Universidade Federal do Triangulo Mineiro, 
      Uberaba, MG, Brazil.
FAU - Rachid, Milene A
AU  - Rachid MA
AD  - Departamento de Patologia Geral, Instituto de Ciencias Biologicas, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Machado, Renes R
AU  - Machado RR
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Coelho, Marcio M
AU  - Coelho MM
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: 
      marciocoelhobh@yahoo.com.br.
LA  - eng
PT  - Journal Article
DEP - 20170423
PL  - Switzerland
TA  - Pharmacol Rep
JT  - Pharmacological reports : PR
JID - 101234999
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9007-81-2 (Freund's Adjuvant)
RN  - TLM2976OFR (Riboflavin)
RN  - X66NSO3N35 (Thiamine)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/administration & dosage/therapeutic use
MH  - Chronic Disease
MH  - Cytokines/genetics/*metabolism
MH  - Dexamethasone/administration & dosage/*therapeutic use
MH  - Drug Therapy, Combination
MH  - Female
MH  - Freund's Adjuvant/*therapeutic use
MH  - Gene Expression Regulation/drug effects
MH  - Inflammation/chemically induced/*drug therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Riboflavin/administration & dosage/*pharmacology
MH  - Thiamine/administration & dosage/*pharmacology
OTO - NOTNLM
OT  - Corticosteroids
OT  - Cytokines
OT  - Inflammation
OT  - Riboflavin
OT  - Thiamine
EDAT- 2017/09/30 06:00
MHDA- 2018/07/31 06:00
CRDT- 2017/09/30 06:00
PHST- 2016/10/11 00:00 [received]
PHST- 2017/01/26 00:00 [revised]
PHST- 2017/04/11 00:00 [accepted]
PHST- 2017/09/30 06:00 [pubmed]
PHST- 2018/07/31 06:00 [medline]
PHST- 2017/09/30 06:00 [entrez]
AID - S1734-1140(16)30272-9 [pii]
AID - 10.1016/j.pharep.2017.04.011 [doi]
PST - ppublish
SO  - Pharmacol Rep. 2017 Oct;69(5):1036-1043. doi: 10.1016/j.pharep.2017.04.011. Epub 
      2017 Apr 23.