PMID- 28959595
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2214-7500 (Print)
IS  - 2214-7500 (Electronic)
IS  - 2214-7500 (Linking)
VI  - 3
DP  - 2016
TI  - Trimellitic anhydride induces low-grade mast cell degranulation without specific 
      IgE.
PG  - 701-707
LID - 10.1016/j.toxrep.2016.09.001 [doi]
AB  - OBJECTIVES: Low-molecular-weight (LMW) substances are known to be causative 
      agents of occupational asthma (OA) and occupational rhinitis (OR). Although most 
      LMW substances are irritants or allergens, some can cause immediate type 
      immunoglobulin E (IgE)-mediated allergic reactions. Trimellitic anhydride (TMA) 
      is one such LMW substance, which is known as an immunological sensitizer. 
      However, the exact molecular biological details of the effects of TMA remain 
      unclear. METHODS: We measured the beta-hexosaminidase release from mast cells after 
      directly exposing the cells to various LMW substances. The tyrosine 
      phosphorylation of whole cellular molecules and the phosphorylation of 
      extracellular signal-regulated kinase (ERK) were assessed by immunoblot assay. 
      RESULTS: Among the LMW substances tested, only TMA induced beta-hexosaminidase 
      release. However, the mast cell degranulation induced by TMA was lower than that 
      induced by an antigen or a calcium ionophore. Moreover, the pattern of tyrosine 
      phosphorylation of whole cellular molecules was quite different between 
      IgE-mediated antigen stimulation and TMA exposure. The TMA effect on mast cells 
      was independent of not only IgE but also Ca(2+) influx. ERK phosphorylation was 
      not detected in mast cells exposed to TMA. CONCLUSIONS: TMA induced mild 
      degranulation of mast cells without IgE, even though the phosphorylation of ERK 
      was not detected. This reaction suggests that TMA affects humans even upon first 
      exposure. Therefore, it is imperative to avoid human exposure to high 
      concentrations of TMA. In order to stop the development of severe asthma in 
      individuals with OR, we need to be able to identify cases of OR caused by TMA as 
      soon as possible.
FAU - Ogi, Kazuhiro
AU  - Ogi K
AD  - Division of Otorhinolaryngology Head and Neck Surgery, Department of Sensory and 
      Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Fukui 
      910-1193, Japan.
FAU - Takabayashi, Tetsuji
AU  - Takabayashi T
AD  - Division of Otorhinolaryngology Head and Neck Surgery, Department of Sensory and 
      Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Fukui 
      910-1193, Japan.
FAU - Yamada, Takechiyo
AU  - Yamada T
AD  - Division of Otorhinolaryngology Head and Neck Surgery, Department of Sensory and 
      Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Fukui 
      910-1193, Japan.
FAU - Sakashita, Masafumi
AU  - Sakashita M
AD  - Division of Otorhinolaryngology Head and Neck Surgery, Department of Sensory and 
      Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Fukui 
      910-1193, Japan.
FAU - Kanno, Masafumi
AU  - Kanno M
AD  - Division of Otorhinolaryngology Head and Neck Surgery, Department of Sensory and 
      Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Fukui 
      910-1193, Japan.
FAU - Narita, Norihiko
AU  - Narita N
AD  - Division of Otorhinolaryngology Head and Neck Surgery, Department of Sensory and 
      Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Fukui 
      910-1193, Japan.
FAU - Fujieda, Shigeharu
AU  - Fujieda S
AD  - Division of Otorhinolaryngology Head and Neck Surgery, Department of Sensory and 
      Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Fukui 
      910-1193, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160907
PL  - Ireland
TA  - Toxicol Rep
JT  - Toxicology reports
JID - 101630272
PMC - PMC5616080
OTO - NOTNLM
OT  - BSA, bovine serum albumin
OT  - DNP, dinitrophenylated
OT  - HMW, high molecular weight
OT  - IL, interleukin
OT  - IgE, immediate immunoglobulin E
OT  - IgE, immunoglobulin E
OT  - LMW, low molecular weight
OT  - Mast cell
OT  - OA, occupational asthma
OT  - OR, occupational rhinitis
OT  - Occupational rhinitis
OT  - PD, piecemeal degranulation
OT  - Respiratory hypersensitivity
OT  - TMA, trimellitic anhydride
OT  - Trimellitic anhydride
EDAT- 2017/09/30 06:00
MHDA- 2017/09/30 06:01
PMCR- 2016/09/07
CRDT- 2017/09/30 06:00
PHST- 2016/03/14 00:00 [received]
PHST- 2016/09/01 00:00 [revised]
PHST- 2016/09/02 00:00 [accepted]
PHST- 2017/09/30 06:00 [entrez]
PHST- 2017/09/30 06:00 [pubmed]
PHST- 2017/09/30 06:01 [medline]
PHST- 2016/09/07 00:00 [pmc-release]
AID - S2214-7500(16)30068-3 [pii]
AID - 10.1016/j.toxrep.2016.09.001 [doi]
PST - epublish
SO  - Toxicol Rep. 2016 Sep 7;3:701-707. doi: 10.1016/j.toxrep.2016.09.001. eCollection 
      2016.